Simultaneous determination of 5α reduced metabolites of testosterone in human plasma

Toscano, Vincenzo; Petrangeli, Elisa; Adamo, Maria; Foli, Sonia; Caiola, Stefania; Sciarra, F

doi:10.1016/0022-4731(81)90033-9

Cited by 33 publications

(6 citation statements)

References 13 publications

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“…Intraprostatic content of T and DHT was determined in duplicate by radioimmunoassay (RIA) in tissue homogenate after extraction with ice-cold acetone and ether and purification on celite microcolumns eluted with isooctane-benzene, 85:15 v/v for DHT and 60:40 v/v for T [23,24]. Specific antibody for T was purchased from Bio-Merieux (Marcy-l'Etoile, Lyon, France) and for DHT from Diagnostic Systems Laboratories, Inc. (Webster, TX).…”

Section: Methodsmentioning

confidence: 99%

Effects of long-term treatment withSerenoa repens (Permixon®) on the concentrations and regional distribution of androgens and epidermal growth factor in benign prostatic hyperplasia

et al. 1998

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Section: Methodsmentioning

confidence: 99%

Effects of long-term treatment withSerenoa repens (Permixon®) on the concentrations and regional distribution of androgens and epidermal growth factor in benign prostatic hyperplasia

et al. 1998

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“…Steroids were assayed by RIA after extraction and celite chromatography. The methods and re-422 COMMENTS producibility have been described elsewhere (20,21).…”

Section: Methodsmentioning

confidence: 99%

Response to a Single Dose of Human Chorionic Gonadotropin in Prepubertal Boys*

Toscano

Balducci

Adamo

et al. 1983

The Journal of Clinical Endocrinology & Metabolism

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In normal men a single dose of hCG induces an increase in plasma testosterone (T) and 17 alpha-hydroxyprogesterone (17 alpha-OHP) 2-4 h after the injection; after 24-36 h a maximum increase in plasma 17 beta-estradiol (E2) and 17 alpha-OHP occurs followed by a second surge in T after 48-96 h. The present investigation focuses on the effect of a single dose of hCG (3500 IU/m2 body surface) on testicular steroid production in 12 boys aged 13 months to 12 yr. Plasma hCG, 17 alpha-OHP, androstenedione (A), T, dihydrotestosterone, and E2 were measured basally and 2, 4, 24, 48, 72, and 96 h after hCG administration. Plasma hCG was measured using a double antibody RIA technique and steroids by RIA after celite chromatography. The results show that hCG peaked 2 h after administration of the hormone and high levels persisted for up until 72 h. Plasma T and dihydrotestosterone increased after 48 h and remained significantly high for another 48 h; 17 alpha-OHP, A, and E2 did not change. These findings show that hCG stimulation in prepubertal boys induces significant production of T without affecting the precursors or aromatization product, in contrast to observation in the adult man, where 17 alpha-OHP, A, and E2 increase significantly. A response comparable to that observed in children has been recorded in adult males with hypogonadotropic hypogonadism.

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“…3) which, it has been claimed, possess some diagnostic usefulness in hirsutism as plasma markers of peripheral androgen metabolism (94,95,104). Less, if any, importance has been attributed to DHT itself, since the plasma concentrations of this hormone poorly reflect the intracellular concentrations (105,106). The process of 5a-reduction also takes place in the skin (107) in which the presence of specific receptors for DHT (108) and E 2 (109) have been documented.…”

Section: Skin Metabolism Of Androgenic Hormones In Idiopathic Hirsutismmentioning

confidence: 95%

Female Hirsutism: Pathophysiological Considerations and Therapeutic Implications*

1984

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Simultaneous determination of 5α reduced metabolites of testosterone in human plasma

Cited by 33 publications

References 13 publications

Effects of long-term treatment withSerenoa repens (Permixon®) on the concentrations and regional distribution of androgens and epidermal growth factor in benign prostatic hyperplasia

Effects of long-term treatment withSerenoa repens (Permixon®) on the concentrations and regional distribution of androgens and epidermal growth factor in benign prostatic hyperplasia

Response to a Single Dose of Human Chorionic Gonadotropin in Prepubertal Boys*

Female Hirsutism: Pathophysiological Considerations and Therapeutic Implications*

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