Simultaneous determination of 6-monoacetylmorphine, morphine and codeine in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection

Gerostamoulos, Dimitri; Crump, Kerryn; McIntyre, Iain M.; Drummer, Olaf H.

doi:10.1016/0378-4347(93)80435-7

Cited by 19 publications

(12 citation statements)

References 6 publications

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“…The cocaine standard curve was prepared in methanol using methanol stock solutions of cocaine and cocaine-d ; 19 samples with different cocaine concentrations (0, 0.01, 0.025, 0.050, 0.1, 0.25, 0.5, 1, 2, 4, 5, 6, 8, 10, 20, 30, 50, 75, 100 g/mL) were used. The internal standard, cocaine-d , was added to each sample resulting in a final concentration of 5 g/mL.…”

Section: Sample Preparationmentioning

confidence: 99%

“…A variety of analytical methods are used in drug screening procedures, such as enzyme multiplied immunoassay techniques (EMIT) [12][13][14], radioimmunoassay (RIA) [15][16][17], high performance liquid chromatography (HPLC) [18][19][20] utilizing a diversity of detectors, thin layer chromatography (TLC) [21][22][23], micellar electrokinetic capillary chromatography [24][25][26] and fluorescence polarization immunoassay (FPIA) [27][28][29]. For urine analysis of cocaine, EMIT seems to be the prevalent choice, with detection limits for BE in urine ca.…”

Section: Introductionmentioning

confidence: 99%

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Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Muddiman

Gusev

Martin

et al. 1996

Fresenius' Journal of Analytical Chemistry

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The direct quantification of cocaine in urine has been achieved using time-of-flight secondary-ion mass spectrometry (TOF-SIMS). The dynamic range of the TOF-SIMS is four orders of magnitude using one internal standard concentration. The limit of detection (LOD) and limit of quantification (LOQ) have been determined to be 3 ng/mL and 10 ng/mL, respectively, for MeOH standards; the LOD and LOQ have been 33 ng/mL and 100 ng/mL, respectively, in urine samples. Coefficients of variation have ranged from 0.7 to 2.7 for the MeOH standards and 2.5 to 8.0 for the urine samples. The use of TOF-SIMS to simultaneously detect multiple drugs in urine is also demonstrated. The advantages and disadvantages of directly quantifying drugs in urine are discussed. Practical considerations regarding the use of matrix-assisted laser desorption/ionization mass spectrometry for multi-component analysis are also presented.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Muddiman

Gusev

Martin

et al. 1996

Fresenius' Journal of Analytical Chemistry

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“…A number of analytical techniques are used for determination of both MR and MAM in postmortem specimens, including GC-MS (Lewis et al, 2005;Wyman et al, 2005;Lowe et al, 2006;Engelhart et al, 2002;Lee et al, 2009;Morley et al, 2007;Avella et al, 2007), LC-MS (Avella et al, 2007;Gergov et al, 2007), LC-MS-MS (Miller et al, 2008;Kronstrand et al, 2004) and HPLC with dual electrochemical and spectrophotometric detection (Gerostamoulos et al, 1993). MR was determined alone in postmortem samples by GC-MS (Kerrigan et al, 2004;Crandall et al, 2006), hybrid quadrupole linear ion-trap LC-MS (Taylor et al, 2009), and HPLC with electrochemical detection (Staub et al, 1990) and fluorescence detection (Skopp et al, 1996;Aderjan et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatographic determination of diacetylmorphine metabolites in urine and postmortem plasma

Hadad¹,

Salam²,

Emara³

2018

Records of Pharmaceutical and Biomedical Sciences

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Determination of drugs of abuse in blood is of great importance in clinical and forensic toxicology. This paper represents a simple and rapid method for the determination of diacetyl morphine metabolites in urine and postmortem plasma specimens using reversed-phase high-performance liquid chromatography with ultraviolet spectrophotometric detection. The proposed method was based on using C18 column with mobile phase consisting of a mixture of acetonitrile-20 mM KH2PO4-diethylamine (10: 90: 0.1, v/v, pH 3.0 adjusted with phosphoric acid). Quantitation was achieved with UV detection at 280 nm using phenylephrine as internal standard. The metabolites were successfully extracted from addict urine and postmortem plasma with good recoveries and reproducibility.

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“…E-mail: mashhadizadeh@tmu.ac.ir selective electrode [8]. Sample preparation techniques prior to instrumental analysis were based on liquid-liquid extraction (LLE) [9] and solid phase extraction (SPE) [10][11][12][13]. Unfortunately, these methods require a large sample volume and are time consuming.…”

Section: Introductionmentioning

confidence: 99%

Cloud point extraction and spectrophotometric determination of codeine in pharmaceutical and biological samples

Mashhadizadeh¹,

Jafari²

2010

JICS

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Cloud point extraction process using non-ionic surfactant, Triton X-114, to extract codeine from aqueous solution was investigated. The method was based on the extraction of codeine and bromothymol blue from acetate buffer media to surfactantrich phase and formed a charge transfer-ion pair complex. The extracted surfactant-rich phase was diluted with ethanol and its absorbance was measured at 430 nm. The effect of different variables such as pH, Triton X-114 concentration, cloud point temperature and time was established. The calibration graph was linear in a wide range of 100 -700 ng ml -1 of codeine with r = 0.998 (n = 7). The detection limit based on three times standard deviation of the blank (3s) was 4.6 ng ml -1 and relative standard deviation (R.S.D) is 2.15% for 500 ng ml -1 codeine (n = 5). The proposed method was applied to the determination of codeine in acetaminophen codeine tablets and blood samples.

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Simultaneous determination of 6-monoacetylmorphine, morphine and codeine in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection

Cited by 19 publications

References 6 publications

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

High-performance liquid chromatographic determination of diacetylmorphine metabolites in urine and postmortem plasma

Cloud point extraction and spectrophotometric determination of codeine in pharmaceutical and biological samples

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