Simultaneous determination of a new gastrointestinal prokinetic agent (HSR-803) and its metabolites in human serum and urine by high-performance liquid chromatography using automated column-switching

Takahara, Eiji; Fukuoka, Hideaki; Takagi, Takeshi; Nagata, Osamu; Kobayashi, Hideo

doi:10.1016/0378-4347(92)80191-r

Cited by 9 publications

(1 citation statement)

References 12 publications

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“…The limitation of this method is its use of a high ratio of buffer solution in the mobile phase that can cause damage to the reverse phase column and the elution time of itopride is more than 10 min. Takahara et al [5] has reported an HPLC method for simultaneous determination of itopride (HSR-803) and its metabolites in human serum and urine using automated column switching.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic determination of itopride hydrochloride in the presence of its degradation products

Kaul¹,

Agrawal²,

Maske³

et al. 2005

J of Separation Science

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Two sensitive and reproducible methods are described for the quantitative determination of itopride hydrochloride (IH) in the presence of its degradation products. The first method is based on HPLC separation on a reversed phase Kromasil column [C18 (5-microm, 25 cm x 4.6 mm, ID)] at ambient temperature using a mobile phase consisting of methanol and water (70:30, v/v) adjusted to pH 4.0 with orthophosphoric acid with UV detection at 258 nm. The flow rate was 1.0 mL per min with an average operating pressure of 180 kg/cm2. The second method is based on HPTLC separation on silica gel 60 F254 using toluene:methanol:chloroform:10% ammonia (5.0:3.0:6.0:0.1, v/v/v/v) as mobile phase at 270 nm. The analysis of variance (ANOVA) and Student's t-test were applied to correlate the results of IH determination in dosage form by means of HPLC and HPTLC methods. The drug was subjected to acid and alkali hydrolysis, oxidation, dry heat, wet heat treatment, UV, and photodegradation. The proposed HPLC method was utilized to investigate the kinetics of the acidic, alkaline, and oxidative degradation processes at different temperatures and the apparent pseudo-first-order rate constant, half-life, and activation energy were calculated. In addition the pH-rate profile of degradation of IH in constant ionic strength buffer solutions in the pH range 2-11 was studied.

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Section: Introductionmentioning

confidence: 99%