Simultaneous Determination of Acetaminophen and Phenobarbital in Suppositories by Partial Least-Squares Spectrophotometric Calibration

Optimisation and validation of a rapid and efficient microemulsion liquid chromatographic (MELC) method for the determination of paracetamol (acetaminophen) content in a suppository formulation

“…The target of this study, a paracetamol suppository was chosen because suppositories generally require a number of sample extraction and/or pre-treatment steps before the analysis can be performed [7][8][9]11] due to the hydrophobic bulk ingredient in the preparation. Sample preparation was shown to be extremely rapid compared to the reference method and required a single step of dissolving the suppository sample in the microemulsion.…”

“…Various methods such as calorimetry [7], spectrophotometry [8] and HPLC with inverse supercritical extraction [9] have been reported for the assay of paracetamol in suppositories. The latter method reported an improvement in analysis times compared to US Pharmacopoeia (1998) [10] method but still required a number of time consuming extraction steps before HPLC analysis was possible.…”

Determination of paracetamol: Historical evolution

“…Bozdogan et al [121,122] discussed the simultaneous spectrophotometric determination of acetaminophen and phenobarbital and paracetamol, metamizol sodium and caffeine, respectively, with the aid of PLS. Ni et al [123] presents an alternative method based on kinetic spectrophotometry for the simultaneous determination of acetaminophen and phenobarbital with the aid of chemometric approaches.…”

Second order advantage obtained by spectroelectrochemistry along with novel carbon nanotube modified mesh electrode: Application for determination of acetaminophen in Novafen samples

“…The development of analytical techniques for rapid analysis of paracetamol is important for quality and medical control. Many methods have been described for determination of acetaminophen including electrochemistry [5,6], spectrophotometry [7,8], liquid chromatography (LC) [9], chemiluminescence [10,11], amperometric biosensors [12,13] and FTIR-spectrometry [14]. These proposed methods often require complicated operations of pretreatment or expensive instruments which prompt us to develop a chemometrics method for the determination of acetaminophen.…”