Simultaneous determination of aflatoxin B1 and ochratoxin A in licorice roots and fritillary bulbs by solid-phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry

Wang, Lizhi; Wang, Zhen; Gao, Weiwei; Chen, Juan; Yang, Meihua; Kuang, Ying; Huang, Linfang; Chen, Shilin

doi:10.1016/j.foodchem.2012.11.066

Cited by 70 publications

(23 citation statements)

References 20 publications

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“…The ion pairs at m/z 404/358 and m/z 313/285 were employed to analyze OTA and AFB1, respectively. The limits of detection (LOD) and the limits of quantification (LOQ) were less than 0.024 and 0.095 µg/kg, respectively [33]. …”

Section: Methodsmentioning

confidence: 99%

Identification of Ochratoxin A Producing Fungi Associated with Fresh and Dry Liquorice

et al. 2013

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The presence of fungi on liquorice could contaminate the crop and result in elevated levels of mycotoxin. In this study, the mycobiota associated with fresh and dry liquorice was investigated in 3 producing regions of China. Potential toxigenic fungi were tested for ochratoxin A (OTA) and aflatoxin B1 (AFB1) production using liquid chromatography/mass spectrometry/mass spectrometry. Based on a polyphasic approach using morphological characters, β-tubulin and RNA polymerase II second largest subunit gene phylogeny, a total of 9 genera consisting of 22 fungal species were identified, including two new Penicillium species (Penicillium glycyrrhizacola sp. nov. and Penicillium xingjiangense sp. nov.). The similarity of fungal communities associated with fresh and dry liquorice was low. Nineteen species belonging to 8 genera were detected from fresh liquorice with populations affiliated with P. glycyrrhizacola, P. chrysogenum and Aspergillus insuetus comprising the majority (78.74%, 33.33% and 47.06% of total) of the community from Gansu, Ningxia and Xinjiang samples, respectively. In contrast, ten species belonging to 4 genera were detected from dry liquorice with populations affiliated with P. chrysogenum, P. crustosum and Aspergillus terreus comprising the majority (64.00%, 52.38% and 90.91% of total) of the community from Gansu, Ningxia and Xinjiang samples, respectively. Subsequent LC/MS/MS analysis indicated that 5 fungal species were able to synthesize OTA in vitro including P. chrysogenum, P. glycyrrhizacola, P. polonicum, Aspergillus ochraceus and A. westerdijkiae, the OTA concentration varied from 12.99 to 39.03 µg/kg. AFB1 was absent in all tested strains. These results demonstrate the presence of OTA producing fungi on fresh liquorice and suggest that these fungi could survive on dry liquorice after traditional sun drying. Penicillium chrysogenum derived from surrounding environments is likely to be a stable contributor to high OTA level in liquorice. The harvesting and processing procedure needs to be monitored in order to keep liquorice free of toxigenic fungi.

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Section: Methodsmentioning

confidence: 99%

Identification of Ochratoxin A Producing Fungi Associated with Fresh and Dry Liquorice

et al. 2013

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“…Currently, many chromatographic based methods such as high performance liquid chromatography-fluorescence detection (HPLC-FL) and liquid chromatography-mass spectrometry (LC-MS) [8][9][10][11] have been proposed for AFs detection in food and feedstuffs. However, these methods are costly, laborious, time-consuming, and require complicated instruments.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the complexity of real matrices often makes it difficult to effectively extract AFs from the sample [18]. Solid phase extraction (SPE) is the mainly used method to isolate AFs from different food and feed matrices [8,19,20] and the simplicity of work-up procedures is an important forensic issue when AFs are likely to be present at low concentrations in samples.…”

Section: Introductionmentioning

confidence: 99%

Surfactant-enhanced spectrofluorimetric determination of total aflatoxins from wheat samples after magnetic solid-phase extraction using modified Fe3O4 nanoparticles

Manafi

Allahyari²,

Pourghazi

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…The most widely used analytical techniques available for CIT and OTA determination are high-performance liquid chromatography (HPLC) with fluorescence detection [3,15,16], liquid chromatography-mass spectrometry (LC-MS) [17][18][19], ultrahigh-performance liquid chromatography -masss pectrometry (UHPLC-MS) [20,21] and other flow or batch methods such as sequential injection analysis (SIA) [22], capillary electrophoresis (CE) [23,24] and ELISA methods [25,26]. A wide spectrum of different methodologies has been proposed for mycotoxins extraction, preconcentration and food sample clean-up.…”

Section: Introductionmentioning

confidence: 99%

A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE–HPLC for determination of ochratoxin A and citrinin in lager beers

et al. 2016

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A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of 100 μL filtered beer samples into an on-line SPE-HPLC system enabled fast and effective sample extraction including separation in less than 6 min. Preconcentration of OTA and CIT from beer samples was performed on an Ascentis Express RP C18 guard column (5 × 4.6 mm), particle size 2.7 μm, with a mobile phase of methanol/0.5% aqueous acetic acid pH 2.8 (30:70, v/v) at a flow rate of 2.0 mL min(-1). The flow switch from extraction column to analytical column in back-flush mode was set at 2.0 min and the separation was performed on the fused-core column Ascentis Express Phenyl-Hexyl (100 × 4.6 mm), particle size 2.7 μm, with a mobile phase acetonitrile/0.5% aqueous acetic acid pH 2.8 in a gradient elution at a flow rate of 1.0 mL min(-1) and temperature of 50 °C. Fluorescence excitation/emission detection wavelengths were set at 335/497 nm. The accuracy of the method, defined as the mean recoveries of OTA and CIT from light and dark beer samples, was in the range 98.3-102.1%. The method showed high sensitivity owing to on-line preconcentration; LOQ values were found to be 10 and 20 ng L(-1) for OTA and CIT, respectively. The found values of OTA and CIT in all tested light, dark and wheat beer samples were significantly below the maximum tolerable limits (3.0 μg kg(-1) for OTA and 2000 μg kg(-1) for CIT) set by the European Union.

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Simultaneous determination of aflatoxin B1 and ochratoxin A in licorice roots and fritillary bulbs by solid-phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry

Cited by 70 publications

References 20 publications

Identification of Ochratoxin A Producing Fungi Associated with Fresh and Dry Liquorice

Identification of Ochratoxin A Producing Fungi Associated with Fresh and Dry Liquorice

Surfactant-enhanced spectrofluorimetric determination of total aflatoxins from wheat samples after magnetic solid-phase extraction using modified Fe3O4 nanoparticles

A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE–HPLC for determination of ochratoxin A and citrinin in lager beers

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