Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor

Pagkali, Varvara; Petrou, Panagiota; Makarona, Eleni; Peters, Jeroen; Haasnoot, W.; Jobst, G.; Moser, I.; Gajos, Katarzyna; Budkowski, Andrzej; Economou, Anastasios; Μισιακός, Κωνσταντίνος; Raptis, Ioannis; Kakabakos, Sotirios

doi:10.1016/j.jhazmat.2018.07.080

Cited by 46 publications

(23 citation statements)

References 38 publications

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“…Slight agitation is needed followed by centrifugation in order to facilitate the sedimentation of the sample. For the extraction of the contaminated beer sample a method previously developed by Pagkali et al was used [27], since the r-Biopharm protocol was not developed for the analysis of liquid samples. The degassed beer sample was simply diluted 8 times with the r-Biopharm extraction.…”

Section: Sample Preparationmentioning

confidence: 99%

Direct analysis of lateral flow immunoassays for deoxynivalenol using electrospray ionization mass spectrometry

2020

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Lateral flow immunoassays (LFIAs) are widely used for rapid food safety screening analysis. Thanks to simplified protocols and smartphone readouts, LFIAs are expected to be increasingly used on-site, even by non-experts. As a typical follow-up in EU regulatory settings, suspect samples are sent to laboratories for confirmatory analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). However, re-analysis by LC-MS/MS is laborious and time-consuming. In this work, an identification LFIA (ID-LFIA) approach followed by quadrupole-orbitrap MS or triple quadrupole MS/MS analysis is presented. As a proof of concept, a dedicated ID-LFIA strip was developed for the mycotoxin deoxynivalenol (DON) following its initial screening by a commercial smartphone LFIA. The ID-LFIA strip can be simply immersed in the same sample extract used for the smartphone LFIA screening, and next, DON is retrieved from the monoclonal antibody with a dissociation solution consisting of methanol/ammonia. The solution thus obtained was analyzed directly in MS in order to rapidly confirm the presence of DON and any cross-reacting species. The protocol developed is capable of coping with severe ion suppression caused by LFIA buffers and nitrocellulose substrate residues. Initial analysis of blank, spiked, and incurred samples showed that the newly developed ID-LFIA-MS method was able to confirm the presence or absence of mycotoxins in the samples previously analyzed by LFIA and also differentiate between DON and DON 3-glucoside yielding the positive screening result. The concept and technique developed are envisaged to complement on-site screening and confirmation of any low molecular weight contaminant in future food control frameworks.

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Section: Sample Preparationmentioning

confidence: 99%

Direct analysis of lateral flow immunoassays for deoxynivalenol using electrospray ionization mass spectrometry

2020

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“…In addition to single analyte determinations, multiplexed determinations were also performed through appropriate functionalization of the different MZIs of a single chip with the different biomolecules. Thus, the multiplexed detection of mycotoxins, aflatoxin B 1 , fumonisin B 1 , and deoxynivalenol in beer samples was presented following a competitive immunoassay format with LODs of 0.8, 5.6, and 24 ng/mL for aflatoxin B 1 , fumonisin B 1 , and deoxynivalenol, respectively, and an overall assay duration of 12 min [137]. Through the analysis of beers of different types and origin, the sensor performance was compared with those provided by established instrumental laboratory methods [138].…”

Section: Monolithically Integrated Broad-band Optoelectronic Transducersmentioning

confidence: 99%

Label-Free Biosensors Based onto Monolithically Integrated onto Silicon Optical Transducers

2018

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The article reviews the current status of label-free integrated optical biosensors focusing on the evolution over the years of their analytical performance. At first, a short introduction to the evanescent wave optics is provided followed by detailed description of the main categories of label-free optical biosensors, including sensors based on surface plasmon resonance (SPR), grating couplers, photonic crystals, ring resonators, and interferometric transducers. For each type of biosensor, the detection principle is first provided followed by description of the different transducer configurations so far developed and their performance as biosensors. Finally, a short discussion about the current limitations and future perspectives of integrated label-free optical biosensors is provided.

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“…Moreover, the light coupled to the device was just a little fraction of the incident light [116]. The only reliable solution provided so far to this problem was the implementation as light sources of silicon light emitting diodes (LED) integrated onto the same silicon chip with planar silicon nitride waveguides [117,[119][120][121][122][123][124][125][126][127][128][129][130][131]. The LEDs were silicon avalanche diodes that emitted polychromatic light covering all the visible and near IR spectrum when biased beyond their breakdown point [118].…”

Section: Monolithically Integrated Broad-band Optoelectronic Transducersmentioning

confidence: 99%

“…In addition to single analyte determinations, multiplexed determinations were also performed through appropriate functionalization of the different MZIs of a single chip with the different biomolecules. Thus, the multiplexed detection of mycotoxins, aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples was presented following a competitive immunoassay format with LODs of 0.8, 5.6 and 24 ng/ml for aflatoxin B1, fumonisin B1 and deoxynivalenol, respectively, and an overall assay duration of 12 min [130]. Through analysis of beers of different types and origin, the sensor performance was compared with those provided by established instrumental laboratory methods [130].…”

Section: Monolithically Integrated Broad-band Optoelectronic Transducersmentioning

confidence: 99%

“…Thus, the multiplexed detection of mycotoxins, aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples was presented following a competitive immunoassay format with LODs of 0.8, 5.6 and 24 ng/ml for aflatoxin B1, fumonisin B1 and deoxynivalenol, respectively, and an overall assay duration of 12 min [130]. Through analysis of beers of different types and origin, the sensor performance was compared with those provided by established instrumental laboratory methods [130]. In addition, the simultaneous, label-free immunochemical detection of four allergens, bovine milk protein, peanut protein, soy protein, and gliadin, in 6.5 min, with LODs of 0.04, 1.0, 0.80, and 0.10 μg/mL, respectively, has been reported.…”

Section: Monolithically Integrated Broad-band Optoelectronic Transducersmentioning

confidence: 99%

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Label-Free Biosensors Based Onto Monolithically Integrated Onto Silicon Optical Transducers

Angelopoulou¹,

Kakabakos²,

Petrou³

2018

Preprint

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The article reviews the current status of label-free integrated optical sensors focusing on the evolution over the years of their analytical performance. At first a short introduction to the evanescent wave optics is provided followed by detailed description of the main categories of label-free optical sensors including sensors based on surface plasmon resonance (SPR), grating coupler, photonic crystals, ring resonators and interferometric transducers. After a short reference to SPR techniques, including localized SPR, integrated optical sensors i.e., grating-couplers, interferometers, photonic crystals, microring resonators are reviewed. For each type of sensor, the detection principle is first provided followed by description of the different transducer configurations so far developed and their performance as biosensors. Finally, a short discussion about the current limitations and future perspectives of integrated label-free biosensors optical sensors is provided

show abstract

Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor

Cited by 46 publications

References 38 publications

Direct analysis of lateral flow immunoassays for deoxynivalenol using electrospray ionization mass spectrometry

Direct analysis of lateral flow immunoassays for deoxynivalenol using electrospray ionization mass spectrometry

Label-Free Biosensors Based onto Monolithically Integrated onto Silicon Optical Transducers

Label-Free Biosensors Based Onto Monolithically Integrated Onto Silicon Optical Transducers

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