Simultaneous determination of aflatoxins B1, B2, G1, G2inFructus Bruceaeby high-performance liquid chromatography with online postcolumn photochemical derivatization

Cao, Jiliang; Zhou, Shujun; Kong, Weijun; Ma, Xiaochi; Yang, Meihua; Wan, Li; Yang, Shihai

doi:10.1002/jssc.201400501

Cited by 21 publications

(11 citation statements)

References 33 publications

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“…AFs have been detected using HPLC with fluorescence detection (FLD) by the enhancement of aflatoxins G1 (AFG1) and B1 (AFB1) signals with pre‐ or post‐column derivatization methods . Due to the lack of natural fluorescence in DON, its determination in food and feed was based mainly on the use of HPLC or GC coupled with MS , while HPLC with diode array detection (DAD) is rarely used .…”

Section: Introductionmentioning

confidence: 99%

Optimized and validated high-performance liquid chromatography method for the determination of deoxynivalenol and aflatoxins in cereals

Skendi

Irakli²,

Παπαγεωργίου

2016

J. Sep. Science

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A simple, sensitive and accurate analytical method was optimized and developed for the determination of deoxynivalenol and aflatoxins in cereals intended for human consumption using high-performance liquid chromatography with diode array and fluorescence detection and a photochemical reactor for enhanced detection. A response surface methodology, using a fractional central composite design, was carried out for optimization of the water percentage at the beginning of the run (X1, 80-90%), the level of acetonitrile at the end of gradient system (X2, 10-20%) with the water percentage fixed at 60%, and the flow rate (X3, 0.8-1.2 mL/min). The studied responses were the chromatographic peak area, the resolution factor and the time of analysis. Optimal chromatographic conditions were: X1 = 80%, X2 = 10%, and X3 = 1 mL/min. Following a double sample extraction with water and a mixture of methanol/water, mycotoxins were rapidly purified by an optimized solid-phase extraction protocol. The optimized method was further validated with respect to linearity (R(2) >0.9991), sensitivity, precision, and recovery (90-112%). The application to 23 commercial cereal samples from Greece showed contamination levels below the legally set limits, except for one maize sample. The main advantages of the developed method are the simplicity of operation and the low cost.

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Section: Introductionmentioning

confidence: 99%

Optimized and validated high-performance liquid chromatography method for the determination of deoxynivalenol and aflatoxins in cereals

Skendi

Irakli²,

Παπαγεωργίου

2016

J. Sep. Science

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“…Hence our understanding of these materials should be strengthened to develop aflatoxin (AF) detection methods to ensure the safety of TCMs. Currently, detection methods exist for the monitoring of AF contamination in some TCMs 9 10 such as licorice roots, fritillary bulbs, Fructus Bruceae , but comprehensive and systematic investigations on TCMs are lacking.…”

mentioning

confidence: 99%

Analysis of aflatoxins in traditional Chinese medicines: Classification of analytical method on the basis of matrix variations

Zhao

Zhang

Tan

et al. 2016

Sci Rep

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A classification system for analytical methods was developed for the first time to determine the presence of aflatoxins B1, B2, G1 and G2 in traditional Chinese medicines (TCMs) based on different matrix types using ultra-performance liquid chromatography–tandem mass spectrometry. A useful characteristic of the approach was that the TCMs could be systematically divided into four categories (i.e., volatile oils, proteins, polysaccharides and fatty oils) depending on the matrix types. The approach concluded that different types of TCMs required different optimal sample preparation procedures. Based on the optimized analytical conditions, the limits of detection and quantification, average recoveries and linearity of four aflatoxins were determined and conformed to research limits. Of 22 TCMs samples, 14 samples were contaminated with at least one type aflatoxin at concentrations ranging from 0.2 to 7.5 μg/kg, and the average contents of aflatoxins were significantly different for the different matrix types. Moreover, we found a potential link between the contamination levels of aflatoxins and matrix types. TCMs containing fatty oils were the most susceptible to contamination by aflatoxins and followed by TCMs containing polysaccharides and proteins; TCMs containing abundant amounts of volatile oils were less prone to contamination.

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“…At present, the main technologies regarding the determination of mycotoxins are based on chromatography, such as HPLC [89] and LC-MS/MS [90]. The methods of sample extraction include magnetic solid-phase extraction (MSPE) [91], solid-phase extraction (SPE) [92], and liquid-liquid extraction (LLE) [93].…”

Section: Detection Methods Of Mycotoxinsmentioning

confidence: 99%

Predominant Mycotoxins, Pathogenesis, Control Measures, and Detection Methods in Fermented Pastes

Zhao

Wang

Chen

et al. 2020

Toxins

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Fermented pastes are some of the most popular traditional products in China. Many studies reported a strong possibility that fermented pastes promote exposure to mycotoxins, including aflatoxins, ochratoxins, and cereulide, which were proven to be carcinogenic and neurotoxic to humans. The primary mechanism of pathogenicity is by inhibiting protein synthesis and inducing oxidative stress using cytochrome P450 (CYP) enzymes. The level of mycotoxin production is dependent on the pre-harvest or post-harvest stage. It is possible to implement methods to control mycotoxins by using appropriate antagonistic microorganisms, such as Aspergillus niger, Lactobacillus plantarum, and Saccharomyces cerevisiae isolated from ordinary foods. Also, drying products as soon as possible to avoid condensation or moisture absorption in order to reduce the water activity to lower than 0.82 during storage is also effective. Furthermore, organic acid treatment during the soaking process reduces toxins by more than 90%. Some novel detection technologies based on magnetic adsorption, aptamer probes, and molecular-based methods were applied to rapidly and accurately detect mycotoxins in fermented pastes.

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Simultaneous determination of aflatoxins B₁, B₂, G₁, G₂inFructus Bruceaeby high-performance liquid chromatography with online postcolumn photochemical derivatization

Cited by 21 publications

References 33 publications

Optimized and validated high-performance liquid chromatography method for the determination of deoxynivalenol and aflatoxins in cereals

Optimized and validated high-performance liquid chromatography method for the determination of deoxynivalenol and aflatoxins in cereals

Analysis of aflatoxins in traditional Chinese medicines: Classification of analytical method on the basis of matrix variations

Predominant Mycotoxins, Pathogenesis, Control Measures, and Detection Methods in Fermented Pastes

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