Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography

Balcells, J.; Guada, J.A.; Peíró, José M.; Parker, David S.

doi:10.1016/0378-4347(92)80517-t

Cited by 255 publications

(149 citation statements)

References 13 publications

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“…The infused doses were calculated to increase two-(phase 1: 0, 6, 12 and 18 g RNA per day) or threefold (phase 2: 0, 12, 24 and 36 g RNA per day) the theoretical basal flow. Baseline duodenal flow of PB was estimated assuming 11 g microbial protein per MJ FME (Agricultural and Food Research Council, 1993) and 1.2 mmol PB per g microbial nitrogen (N) (Balcells et al, 1992).…”

Section: Methodsmentioning

confidence: 99%

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Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

Mota¹,

Balcells²,

Baber

et al. 2008

Animal

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To determine the endogenous contribution of purine derivatives (PD) to renal excretion and the urinary recovery of duodenal purine bases (PB), five dairy Granadina goats (initial weight 6 s.e.: 38.6 6 2.78 kg) were each fitted with a duodenal infusion catheter. Animals were offered ad libitum a mixed diet (75 : 25; alfalfa hay : concentrate), which was supplied in equal portions every 3 h. To label microbial PB, ( 15 NH 4 ) 2 SO 4 was added to the concentrate. The lower enrichment of urinary PD ( 15 N-allantoin) compared with duodenal PB enrichment confirmed the presence of an endogenous PD fraction (268.5 6 21.98 mmol/kg weight 0.75 or 0.386 of the total PD excretion). The recovery of PD in urine and milk increased linearly in response to increasing amounts of duodenally infused RNA (starting on day 21 after parturition). On average, 0.74 of infused PB from RNA was recovered in urine. Milk PD constituted a minor (,0.01) fraction of the total PD excretion and this fraction decreased as the amount of infused PB increased. Our findings indicate that lactation in goats did not affect the urinary recovery of duodenal PB but increased the endogenous contribution to urinary excretion of PD.

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Section: Methodsmentioning

confidence: 99%

“…PD and creatinine in urine were determined using HPLC (Balcells et al, 1992). PB were analysed in the digesta after perchloric acid hydrolysis following the procedure of Martín Orú e et al (1995).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

Mota¹,

Balcells²,

Baber

et al. 2008

Animal

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“…Uric acid, hypoxanthine and xanthine were analysed according to the method of Balcells et al (1992) with the following modifications. The HPLC consisted of a binary pump, an autosampler, a column oven and a diode-array detector from the Agilent 1100 series (Waldbronn, Germany).…”

Section: Hohenheim Gas Testmentioning

confidence: 99%

Effects of long-term supplementation of chestnut and valonea extracts on methane release, digestibility and nitrogen excretion in sheep

Wischer

Greiling

Boguhn

et al. 2014

Animal

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The long-term effects of adding chestnut (CHE; Castanea sativa) and valonea (VAL; Quercus valonea) tannin-rich extracts to sheep feed were investigated. In Experiment 1, sheep (65 kg BW) were fed 842 g/day of a ryegrass-based hay. The control-treated animals (CON) received 464 g/day of concentrate, and tannin-treated animals received the same amount of concentrate additionally containing 20 g of the respective tannin-rich extract. Hay and concentrates were offered together in one meal. After the onset of treatment, methane release was measured in respiration chambers for 23.5-h intervals (nine times) in a 190-days period. Faeces and urine were collected three times (including once before the onset of the tannin treatment) to assess digestibility and urinary excretion of purine derivatives. Based on the results obtained from Experiment 1, a second experiment (Experiment 2) was initiated, in which the daily tannin dosage was almost doubled (from 0.9 (Experiment 1) to 1.7 g/kg BW 0.75 ). With the exception of the dosage and duration of the treatment (85 days), Experiment 2 followed the same design as Experiment 1, with the same measurements. In an attempt to compare in vitro and in vivo effects of tannin supplementation, the same substrates and tannin treatments were examined in the Hohenheim gas test. In vitro methane production was not significantly different between treatments. None of the tannin-rich extract doses induced a reduction in methane in the sheep experiments. On the 1st day of tannin feeding in both experiments, tannin inclusion tended to decrease methane release, but this trend disappeared by day 14 in both experiments. In balance period 3 of Experiment 1, lower dry matter and organic matter digestibility was noted for tannin treatments. The digestibility of CP, but not NDF or ADF, was reduced in both experiments. A significant shift in N excretion from urine to faeces was observed for both tannin-rich extracts in both experiments, particularly in Experiment 2. In balance period 2 of Experiment 2, an increased intake of metabolisable energy for VAL was observed. The urinary excretion of purine derivatives was not significantly different between treatments, indicating that microbial protein synthesis was equal for all treatments. Thus, we concluded that both tannin-rich extracts temporary affect processes in the rumen but did not alter methane release over a longer period.

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“…Creatinine and allantoin concentration in urine samples was determined by HPLC following the technique described by Balcells et al (1992).…”

Section: Effect Of Grinding Size and Oil Addition On Nutritive Value mentioning

confidence: 99%

Effect of grinding size and sunflower oil addition on intake, digestibility, rumen function and microbial protein synthesis in sheep fed a dry total mixed ration

Pérez-Torres

Sierra

Vega

et al. 2011

Span. j. agric. res.

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An experiment was carried out in which the effect of grinding a dry total mixed ration, based on barley straw, through 6 or 10 mm sieves on intake, digestibility, rumen function and microbial protein synthesis in sheep was assessed. The effect of including 1% sunflower oil as binder was also explored. None of the variables studied was affected by grinding size, hence the 10 mm sieve was recommended due to its lower cost in terms of energy consumed. On the con- (1%) is then recommended as binder, although a more economical option than sunflower oil should be explored due to its high cost.Additional keywords: feeding costs; particle size. ResumenEfecto del tamaño de molienda y de la adición de aceite de girasol sobre la ingestión, digestibilidad, fermentación ruminal y síntesis de proteína microbiana en ovejas alimentadas con una ración integral seca Se llevó a cabo un experimento en el que se estudió el efecto del tamaño de molienda (a través de cribas de 6 ó 10 mm de diámetro de malla) de una ración integral seca, constituida mayoritariamente por paja de cebada, y administrada a ovejas adultas, vacías y secas, sobre la ingestión, digestibilidad, características de la fermentación ruminal y síntesis de proteína microbiana. También se estudió el efecto, sobre las mismas variables, de la inclusión de un 1% de aceite de girasol utilizado como aglomerante. El tamaño de molienda no afectó a ninguno de los resultados, por lo que se recomienda la utilización de la criba de 10 mm, en virtud de su menor coste energético. Por el contrario, la adición de un 1% de aceite provocó un aumento significativo de la concentración de amoniaco en el rumen (83 vs. 161 mg L -1 ; p = 0,002), de las degradabilidades potenciales de la materia seca (0,65 vs. 0,75; p = 0,0037) ; p = 0,046) y de la degradabilidad efectiva (0,63 vs. 0,73; p = 0,010) de esta última. Por tanto, se recomienda la adición de un 1% de aceite como aglomerante, aunque dado el alto precio del de girasol es necesario buscar opciones más económicas.Palabras clave adicionales: costes de alimentación; tamaño de partícula.

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Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography

Cited by 255 publications

References 13 publications

Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

Effects of long-term supplementation of chestnut and valonea extracts on methane release, digestibility and nitrogen excretion in sheep

Effect of grinding size and sunflower oil addition on intake, digestibility, rumen function and microbial protein synthesis in sheep fed a dry total mixed ration

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