Simultaneous determination of amiloride and hydrochlorothiazide in plasma by reversed-phase high-performance liquid chromatography

Liquid extraction and HPLC-DAD assay of hydrochlorothiazide from plasma for a bioequivalence study at the lowest therapeutic dose

“…The influence of pH was considered important given the wide discrepancy of optimal pH values reported by other authors (9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Samples of an aqueous solution of HCT were made in different buffers (phosphate, acetate, borate) having pH values of 3.8, 6, 7.5, 9 and extracted with ethyl acetate.…”

“…has been considered. Several such methods for HCT have been proposed in the literature (9)(10)(11)(12)(13)(14)(15)(16)(17)(18), but there are large differences between authors with respect to optimal conditions. In particular, specific conditions associated with the evaluation of the bioequivalence of products containing 25 mg HCT are poorly considered.…”

Sensitive quantification of chosen drugs by reversed-phase chromatography with electrochemical detection at a glassy carbon electrode

“…Samples containing relatively high levels of metoclopramide [13][14][15], hydrochlorothiazide [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], imipramine [34][35][36][37][38][39][40][41][42][43][44][45] and diclofenac potassium [46][47][48][49][50][51][52][53][54][55][56][57][58] can be analysed using UV detection. Although, these methods did not present sufficient accuracy, especially at concentrations below 10 ng/ml.…”

Development and Validation of RP-HPLC Method for Determination of Hydrochlorothiazide, Amiloride Hydrochloride and Related Impurities in Bulk and Pharmaceutical Dosage Forms

“…CTZ shows diuretic action like HCZ 5 but HCZ is eight time as potent as CTZ, has prolonged duration of action and less toxic than CTZ 6 , besides CTZ is incompletely and variably absorbed compared to HCZ 5 . To the best of our knowledge, different methods for simultaneous determination of HCZ and AMH together were developed such as spectrophotometric [7][8][9][10][11] , reversed phase high performance liquid chromatography (RP-HPLC) [11][12][13][14][15][16][17][18][19] , thin layer chromatographic (TLC)-densitometric 8,20 ,UV/ chemometric [21][22][23] , capillary electrophoresis 24 and differential pulse polarographic 25 methods. Furthermore HCZ and AMH were assayed in presence of HCZ impurities, CTZ and DSA by HPTLC method 26 Levels of impurities and degradation products in pharmaceutical products were confined according to recommendations issued by International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) and pharmacopeias due to prospect of their side and toxic effects therefore a method for their simultaneous identification and determination must be sufficiently selective 27 .…”