Simultaneous Determination of Amlodipine Besylate and Benazepril Hydrochloride in Pharmaceutical Dosage Form by LC

Kasawar, Gajanan B.; Farooqui, Mazahar

doi:10.2116/analsci.25.1495

Cited by 13 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors concluded that this method is accurate, specific, precise and sensitive for the determination of amlodipine and benazepril in their capsules dosage. This method is suitable for determination of both drugs in their pharmaceutical dosage as the method is comparably easy and economical as the solvent consumption is very low [31]. Barman and team developed and validated reversed phase high performance liquid chromatography for the determination of amlodipine and atenolol in pharmaceutical dosage forms.…”

Section: 42mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

View full text Add to dashboard Cite

Section: 42mentioning

confidence: 99%

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Helaluddin¹,

Alaama²,

Sarker³

et al. 2017

J. CleanWAS

View full text Add to dashboard Cite

“…The LC methods [4][5][6] reported for the assay of AMD and BEZ in combined pharmaceutical dosage form show lower sensitivity as compared to the proposed method and consequently generates a large amount of solvent (acetonitrile) waste, which may adversely affect the environment, therefore demands expensive disposal process. Further, the absence of an official pharmacopoeial method for estimation of combination of AMD and BEZ motivated the authors to attempt, and to develop a stability indicating HPLC method for the estimation of AMD and BEZ which is simple, rapid, less expensive and environment friendly.…”

Section: Introductionmentioning

confidence: 99%

“…Few analytical methods for the determination of amlodipine besylate and benazepril hydrochloride in combined dosage forms including RP-HPLC [4][5][6], spectrophotometry [6,7] and HPTLC [8] have been reported. Several methods have been reported for the determination of amlodipine with other drugs by UV-spectrophotometry [9][10][11][12][13], dual wavelength spectrophotometry [14,15], derivative spectrophotometry [16,17], kinetic spectrophotometry [18], HPLC with UV detection [17,[19][20][21][22][23][24][25][26][27], HPLC with PDA detection [28][29][30], HPLC with fluorescence detection [31], spectrofluorimetry [32], HPTLC [33] and capillary electrophoresis [34] and amlodipine alone by visible spectrophotometry [35,36] and voltammetry [37] from pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

A Validated Stability-Indicating Liquid Chromatographic Method for the Simultaneous Determination of Amlodipine and Benazepril in Capsules Dosage Form

2014

Can Chem Trans

View full text Add to dashboard Cite

A simple, sensitive, reproducible and specific stability indicating high performance liquid chromatographic method for the simultaneous estimation of amlodipine besylate (AMD) and benazepril hydrochloride (BEZ) in their combined drug product was developed and validated. Risperidone (RIS) was used as internal standard. AMD and BEZ were separated and quantitated on a Hichrom 5 C8 column (250 × 4.6 mm i.d., 5 μm particle size) using a mixture of methanol-formic acid 0.1 M (63:37, v/v) as a mobile phase and at a flow rate of 0.9 mL min-1. The retention times were about 3.7, 5.3 and 7.3 min for RIS, BEZ and AMD, respectively. Quantification was achieved with an UV detector at 242 nm over the concentration ranges of 0.85-807.0 μg mL-1 for BEZ and 0.32-785.0 μg mL-1 for AMD. The limits of detection were 0.18 and 0.12 μg mL-1 for BEZ and AMD, respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. AMD and BEZ stock solutions were subjected to different stress conditions. The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values. Stressed samples were assayed using developed HPLC method. The method is also demonstrated to be robust, because it is resistant to small variations of chromatographic variables such as pH, mobile phase composition and flow rate. The proposed method is found to be very sensitive and accurate for the determination of AMD and BEZ in combined capsule dosage form.

show abstract

“…A review of the literature revealed that several analytical techniques have been reported for the determination of the content of AML and its impurities, mainly based on LC techniques. The compendial LC methods for related substances determination of AML (6,7), as well as the reported HPLC methods in the literature (8)(9)(10)(11)(12)(13)(14), are based mainly on mobile phases containing hazardous solvents such as acetonitrile (ACN) or methanol (MeOH), with relatively long elution times of 40 minutes on average.…”

Section: Introductionmentioning

confidence: 99%

Green RP-HPLC method for impurity profile of amlodipine in tablets

Gigopulu,

Godzo,

Atanasovska

et al. 2024

Arhiv za farmaciju

View full text Add to dashboard Cite

Increased awareness of nature preservation has encouraged the introduction of the green analytical chemistry (GAC) practice concepts concerning several important aspects, including sustainable development, environmental impact, and minimum waste. The aim of this research was to contribute to the implementation of this approach for the pharmaceutical industry while retaining the crucial aspects and strict requirements of quality control of medicines. Therefore, an ethanolbased, green and robust high-performance liquid chromatography (HPLC) method for the determination of related substances of amlodipine (AML) in film-coated tablets was developed and optimized using the Design of Experiments (DoE). The chromatographic separation was performed on an RP-select B column (250 x 4.0 mm, 5 mm), using a mixture of 0.04 M sodium dihydrogen phosphate monohydrate (pH 4.0) and ethanol (60:40 % v/v) as a mobile phase. The optimized conditions provided the separation of two specified impurities (impurity D and impurity F). The selectivity of the method was confirmed using forced degradation studies. The Analytical Eco-scale approach and AGREE metrics confirmed that the method conforms to the GAC principles. The validated method was successfully applied for the determination of related substances in three samples from the market, demonstrating the applicability of the method in routine analysis.

show abstract

Simultaneous Determination of Amlodipine Besylate and Benazepril Hydrochloride in Pharmaceutical Dosage Form by LC

Cited by 13 publications

References 18 publications

Current Analytical Methods For Amlodipine And Its Formulations: A Review

Current Analytical Methods For Amlodipine And Its Formulations: A Review

A Validated Stability-Indicating Liquid Chromatographic Method for the Simultaneous Determination of Amlodipine and Benazepril in Capsules Dosage Form

Green RP-HPLC method for impurity profile of amlodipine in tablets

Contact Info

Product

Resources

About