Simultaneous Determination of Amoxicillin and Ampicillin in Fish Meat Using High-Performance Liquid Chromatography

Jo, Mi-Ra; Oheunkyung,; Kim, Poong Ho; 이태식,; Yan, Hong; Lee, Hee Jung

doi:10.5657/kfas.2006.39.6.454

Cited by 8 publications

(2 citation statements)

References 2 publications

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“…The accuracy of the analysis methods was confirmed, with recovery rates in the range of 95.8–111.0%. In previous studies using the same derivatization method for olive flounder tissue, recovery rates were in the range 95.3–106.1% (Jo et al ., ), 100.8–103.8% (Son et al ., ), and 101.2–111.1% (Kim et al ., ). Therefore, the sample preparation methods used in this study were reliable and reproducible.…”

Section: Discussionmentioning

confidence: 99%

The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics

Lim

Jung

et al. 2016

Vet Pharm & Therapeutics

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The efficacy of amoxicillin sodium for controlling field and experimental Streptococcus iniae and S. parauberis infections in olive flounder (Paralichthys olivaceus) was evaluated after a single intramuscular administration. Furthermore, the minimal inhibitory concentrations (MIC) against 21 Streptococcus strains were determined. In addition, the pharmacokinetics and residue depletion in olive flounder were investigated. Single intramuscular doses of amoxicillin sodium at 20, 40, 80, and 160 mg/kg b.w. fish significantly reduced cumulative mortality rates to 18.8-31.3% (P < 0.05) for S. iniae and to 5.0-15.0% (P < 0.01) for S. parauberis, whereas the S. iniae- and S. parauberis-infected positive control groups showed cumulative mortality rates of 68.8% and 60.0%, respectively. In a S. parauberis outbreak, amoxicillin sodium reduced the cumulative mortality rate to 7.5% and 4.8% at 20 and 40 mg/kg b.w. fish, respectively, whereas that of the untreated control group was 35.2%. Peak plasma concentrations (C ) following a single intramuscular dose of 40 and 80 mg/kg b.w. fish were 62.64 (T , 1.59 h) and 87.61 (T , 3.02 h) μg/mL, respectively, with large AUC /MIC and C /MIC ratios, and sufficient T > MIC (time for maintaining plasma drug concentration greater than MICs) for S. iniae and S. parauberis. The estimated withdrawal period of amoxicillin sodium from muscle of olive flounder was about 8 days at 40 mg/kg b.w. fish (at 22 ± 1 °C). These results demonstrated a single intramuscular administration of amoxicillin sodium to be effective against streptococcosis in olive flounder.

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Section: Discussionmentioning

confidence: 99%

The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics

Lim

Jung

et al. 2016

Vet Pharm & Therapeutics

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“…The LOD estimated from our data using a conservative model was in the range of 8.9-11.1 lg/kg AMOX. In a previously published study, drug-free olive flounder muscle was spiked with AMOX to the concentrations of 0.5-1 ppm, and drug recovery rate with HPLC, following derivatization of samples, was estimated to be in the range of 106.1-95.3% (Jo et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of amoxicillin trihydrate in cultured olive flounder (Paralichthys olivaceus)

Seo

Jeon

Jung

et al. 2014

Vet Pharm & Therapeutics

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The study was aimed at investigating the pharmacokinetics of amoxicillin trihydrate (AMOX) in olive flounder (Paralichthys olivaceus) following oral, intramuscular, and intravenous administration, using high-performance liquid chromatography following. The maximum plasma concentration (Cmax ), following oral administration of 40 and 80 mg/kg body weight (b.w.), AMOX was 1.14 (Tmax , 1.7 h) and 0.76 μg/mL (Tmax , 1.6 h), respectively. Intramuscular administration of 30 and 60 mg/kg of AMOX resulted in Cmax values of 4 and 4.3 μg/mL, respectively, with the corresponding Tmax values of 29 and 38 h. Intravenous administration of 6 mg/kg AMOX resulted in a Cmax of 9 μg/mL 2 h after administration. Following oral administration of 40 and 80 mg/kg AMOX, area under the curve (AUC) values were 52.257 and 41.219 μg/mL·h, respectively. Intramuscular 30 and 60 mg/kg doses resulted in AUC values of 370.274 and 453.655 μg/mL·h, respectively, while the AUC following intravenous administration was 86.274 μg/mL·h. AMOX bioavailability was calculated to be 9% and 3.6% following oral administration of 40 and 80 mg/kg, respectively, and the corresponding values following intramuscular administration were 86% and 53%. In conclusion, this study demonstrated high bioavailability of AMOX following oral administration in olive flounder.

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Alcohol Metabolizing Activity of Fermented Sea Tangle Juice

Kang¹,

Lee²,

Kim³

2010

Korean Journal of Fisheries and Aquatic Sciences

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Simultaneous Determination of Amoxicillin and Ampicillin in Fish Meat Using High-Performance Liquid Chromatography

Cited by 8 publications

References 2 publications

The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics

The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics

Pharmacokinetics of amoxicillin trihydrate in cultured olive flounder (Paralichthys olivaceus)

Alcohol Metabolizing Activity of Fermented Sea Tangle Juice

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