Simultaneous determination of Avanafil and Dapoxetine in human plasma using liquid chromatography/tandem mass spectrometry (LC-MS/MS) based on a protein precipitation technique

Abstract

A rapid and selective LC-MS/MS method is described for the simultaneous assay of Avanafil and Dapoxetine in human plasma via a protein precipitation (PP) sample preparation technique.

Determination of atypical antipsychotics in human plasma by UPLC-UV with polystyrene nanofibers as a solid-phase extraction sorbent

“…The LLOQ is the lowest amount of an analyte that can be quantitatively determined with acceptable precision and accuracy (the accuracy should be ±20% of nominal and the precision should be ±20% RSD). 30–32 The LLOQs were 1 μg mL −1 . The limit of detection (LOD) refers to the minimum amount of a substance that can be detected.…”

One-drop chemosensing of dapoxetine hydrochloride using opto-analysis by multi-channel μPAD decorated silver nanoparticles: introducing a paper-based microfluidic portable device/sensor toward naked-eye pharmaceutical analysis by lab-on-paper technology

“…Table 1, [49][50][51][52][53][54][55] indicated analytical results of different reports for the detection of DPX as can be seen, various biomedical methods such as HPLC-MS, HPLC-MS/MS, LC-MS/MS, spectrophotometric, uorometric, UPLC-MS, UPLC-MS/MS, have been utilized for the monitoring of DPX.…”

Fast and simple voltammetric sensing of avanafil in the pharmaceutical formulation by using unmodified boron-doped diamond electrode

“…AVN is particularly popular because it operates quickly and has few visible adverse effects compared with the other inhibitors of PDE5 [ 2 ]. AVN, (S)-4-((3-chloro-4-methoxybenzyl) amino)-2-(2-(hydroxymethyl) pyrrolidin-1-yl)-N-(pyrimidin-2-ylmethyl) pyrimidine-5- carboxamide is shown in Scheme 1 [ 3 ]. Specifically, AVN blocks the action of the PDE5 enzyme.…”