Simultaneous determination of berberine in rat blood, liver and bile using microdialysis coupled to high-performance liquid chromatography

Tsai, Pi Lo; Tsai, Tung Hu

doi:10.1016/s0021-9673(02)00378-3

Cited by 60 publications

(28 citation statements)

References 26 publications

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“…According to the chromatograms of berberine in liver and bile (Tsai and Tsai, 2002), the peak areas of berberine and metabolites (suspected temporarily) in the liver were much smaller than those in bile.…”

Section: Tsai and Tsaimentioning

confidence: 97%

“…The validation method of chromatographic analysis for berberine in a microdialysis system has been reported previously (Tsai and Tsai, 2002). Mean unbound berberine blood concentrations-time profiles in dose dependence (10 and 20 mg kg Ϫ1 i.v.)…”

Section: Dose Dependence Of Berberine In Bloodmentioning

confidence: 99%

“…Output data from the detector were integrated via an EZChrom chromatographic data system (Scientific Software, San Ramon, CA). The chromatographic method has previously been reported (Tsai and Tsai, 2002).…”

mentioning

confidence: 99%

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Hepatobiliary Excretion of Berberine

Tsai¹,

Tsai²

2004

Drug Metab Dispos

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147

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This article is available online at http://dmd.aspetjournals.org ABSTRACT:Berberine is a bioactive herbal ingredient isolated from the roots and bark of Berberis aristata or Coptis chinensis. To investigate the detailed pharmacokinetics of berberine and its mechanisms of hepatobiliary excretion, an in vivo microdialysis coupled with highperformance liquid chromatography was performed. In the control group, rats received berberine alone; in the drug-treated group, 10 min before berberine administration, the rats were injected with cyclosporin A (CsA), a P-glycoprotein (P-gp) inhibitor; quinidine, both organic cation transport (OCT) and P-gp inhibitors; SKF-525A (proadifen), a cytochrome P450 inhibitor; and probenecid to inhibit the glucuronidation. The results indicate that berberine displays a linear pharmacokinetic phenomenon in the dosage range from 10 to 20 mg kg ؊1, since a proportional increase in the area under the concentration-time curve (AUC) of berberine was observed in this dosage range. Moreover, berberine was processed through hepatobiliary excretion against a concentration gradient based on the bile-to-blood distribution ratio (AUC bile /AUC blood ); the active berberine efflux might be affected by P-gp and OCT since coadministration of berberine and CsA or quinidine at the same dosage of 10 mg kg ؊1 significantly decreased the berberine amount in bile. In addition, berberine was metabolized in the liver with phase I demethylation and phase II glucuronidation, as identified by liquid chromatography/tandem mass spectrometry. Also, the phase I metabolism of berberine was partially reduced by SKF-525A treatment, but the phase II glucuronidation of berberine was not obviously affected by probenecid under the present study design.

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Section: Tsai and Tsaimentioning

confidence: 97%

Section: Dose Dependence Of Berberine In Bloodmentioning

confidence: 99%

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Hepatobiliary Excretion of Berberine

Tsai¹,

Tsai²

2004

Drug Metab Dispos

Self Cite

147

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show abstract

“…The pharmacokinetics of berberine in plasma, bile, and urine, as well as in the tissues of mice, rats, dogs, rabbits, and humans have been reported using UV spectrophotometry, fluorometry, tritium-labeled berberine, and high-performance liquid chromatography (HPLC)-MS/ MS methods. [23][24][25][26][27][28][29] However, there are few reports on the comparative pharmacokinetics of pure berberine and berberine contained in single-herbs or complex herbal medications.…”

Section: -3mentioning

confidence: 99%

Comparative Pharmacokinetics of Berberine After Oral Administration of Pure Berberine, Coptidis Rhizoma Extract, and Decoctions of Two Different Complex Herbal Formulas to Rats

Chen¹,

Yan

Kim

et al. 2013

Bulletin of the Korean Chemical Society

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“…[5] Analytical methods are reported in literature for the Simultaneous and single molecule particular plant extract [6,7,8] and for whole plant and also studies on plasma has been carried out. [9] we are the first to estimate in multicomponent homeopathic formulation. This work is aimed to develop and validate HPLC method for the determination of Berberine(figure 1) in Homeopathic multicomponent formulation.…”

Section: Introductionmentioning

confidence: 98%

Development and Validation of Analytical Method of Berberine and Its Quantification in Multicomponent Homeopathic Formulations

Mashru¹

2017

WJPR

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Simultaneous determination of berberine in rat blood, liver and bile using microdialysis coupled to high-performance liquid chromatography

Cited by 60 publications

References 26 publications

Hepatobiliary Excretion of Berberine

Hepatobiliary Excretion of Berberine

Comparative Pharmacokinetics of Berberine After Oral Administration of Pure Berberine, Coptidis Rhizoma Extract, and Decoctions of Two Different Complex Herbal Formulas to Rats

Development and Validation of Analytical Method of Berberine and Its Quantification in Multicomponent Homeopathic Formulations

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