Simultaneous Determination of Bioactive Constituents in Garcinia Morella Using Uplc-Q-Tof-MS

Abstract

A simple, precise and robust UPLC-Q-TOF-MS method was developed for the quantification of four marker compounds, namely citric acid derivative, iso garcinol, garcinol and garcimultiflorone-A in Garcinia morella, in order to study the fragmentation pathways of four marker compounds. The developed method was validated according to ICH guidelines in terms of linearity, LOD and LOQ, precision and accuracy. Optimized chromatographic conditions were Waters Acquity BEH C18 (100 X 2.1mm, 1.9 m) column, flow rate of 0.6 mL min-1, 8.0 minutes run time and mobile phase being 0.1 % formic acid in water / acetonitrile. The gradient elution programme 0 (95/5), 0.5 (95/5), 2.50 (5/95), 5.0 (5/95), 6.5 (95/5) and 8.0 (95/5) was carried out on Waters instrument UPLC-Q-TOF-MS. The obtained retention times of citric acid derivative, iso garcinol, garcinol and garcimultiflorone-A were 2.14, 3.86, 4.31 and 5.05 minutes, respectively. LOD and LOQ data was found to be in 0.32-0.56 and 0.99-1.69 mg mL-1, respectively.