Simultaneous determination of bisoprolol and hydrochlorothiazide in human plasma by HPLC coupled with tandem mass spectrometry

Tutunji, Maha F.; Ibrahim, Husam M.; Khabbas, Manal H.; Tutunji, Lara

doi:10.1016/j.jchromb.2009.04.021

Cited by 44 publications

(21 citation statements)

References 24 publications

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“…This however was very minimal and not significant. Hydrochlorothiazide is a bivalent acid, with pKa of 8.6 and 9.9 so mostly unionized at pHs studied [23].…”

Section: Resultsmentioning

confidence: 99%

The effect of pH and ionic strength of dissolution media on in-vitro release of two model drugs of different solubilities from HPMC matrices

Asare-Addo

Conway

Larhrib

et al. 2013

Colloids and Surfaces B: Biointerfaces

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The evaluation of the effects of different media ionic strengths and pH on the release of hydrochlorothiazide, a poorly soluble drug, and diltiazem hydrochloride, a cationic and soluble drug, from a gel forming hydrophilic polymeric matrix were the objectives of this study. The drug to polymer ratio of formulated tablets was 4:1. Hydrochlorothiazide or diltiazem HCl extended release (ER) matrices containing hypromellose (hydroxypropyl methylcellulose (HPMC)) were evaluated in media with a pH range of 1.2-7.5, using an automated USP type III, Bio-Dis dissolution apparatus. The ionic strength of the media was varied over a range of 0-0.4 M to simulate the gastrointestinal fed and fasted states and various physiological pH conditions. Sodium chloride was used for ionic regulation due to its ability to salt out polymers in the midrange of the lyotropic series. The results showed that the ionic strength had a profound effect on the drug release from the diltiazem HCl K100LV matrices. The K4M, K15M and K100M tablets however withstood the effects of media ionic strength and showed a decrease in drug release to occur with an increase in ionic strength.For example, drug release after the 1 hr mark for the K100M matrices in water was 36 %.Drug release in pH 1.2 after 1 hr was 30 %. An increase of the pH 1.2 ionic strength to 0.4 M saw a reduction of drug release to 26 %. This was the general trend for the K4M and K15M matrices as well. The similarity factor f 2 was calculated using drug release in water as a reference. Despite similarity occurring for all the diltiazem HCl matrices in the pH 1.2 media (f2=64-72), increases of ionic strength at 0.2 M and 0.4 M brought about dissimilarity. The hydrochlorothiazide tablet matrices showed similarity at all the ionic strength tested for all polymers (f2= 56-81). The values of f2 however reduced with increasing ionic strengths.DSC hydration results explained the hydrochlorothiazide release from their HPMC matrices.There was an increase in bound water as ionic strengths increased. Texture analysis was employed to determine the gel strength and also to explain the drug release for the diltiazem 3 hydrochloride. This methodology can be used as a valuable tool for predicting potential ionic effects related to in vivo fed and fasted states on drug release from hydrophilic ER matrices.

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“…This however was very minimal and not significant. Hydrochlorothiazide is a bivalent acid, with pKa of 8.6 and 9.9 so mostly unionized at pHs studied [23].…”

Section: Resultsmentioning

confidence: 99%

The effect of pH and ionic strength of dissolution media on in-vitro release of two model drugs of different solubilities from HPMC matrices

Asare-Addo

Conway

Larhrib

et al. 2013

Colloids and Surfaces B: Biointerfaces

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“…A sensitive, specific and selective method has been developed for the simultaneous determination of Bisoprolol and Hydrochlorothiazide in human plasma presented by Tutunji et al [53]. The method employed a state of the art LC-MS/MS operated in the positive and negative ionization switching modes.…”

Section: High Performance Liquid Chromatography Coupled With Mass Spementioning

confidence: 99%

Analytical Techniques for Determination of Hydrochlorothiazide and its Combinations: A Review

Savaj¹,

Patidar²,

Raj³

2015

Int J of Adv in Sci Res

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Hydrochlorothiazide is chemically 6-chloro-1, 1-dioxo-3, 4-dihydro-2H-1, 2, 4-benzothiadiazine-7-sulfonamide. Hydrochlorothiazide is a diuretic drug used for treatment of high blood pressure (hypertension) and accumulation of fluid (edema). It works by blocking salt and fluid reabsorption from the urine in the kidneys, causing increased urine output (dieresis). Hydrochlorothiazide is used to treat excessive fluid accumulation and swelling (edema) of the body caused by heart failure, cirrhosis, chronic kidney failure, corticosteroid medications, and nephrotic syndrome. It can be used alone or in conjunction with other blood pressure lowering medications to treat high blood pressure.This review focuses on the recent developments in analytical techniques for estimation of Hydrochlorothiazide alone or in combinations with other drugs in various biological media like human plasma and urine. This review will critically examine the (a) sample pretreatment method such as solid phase extraction (SPE), (b) separation methods such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC), ultra performance liquid chromatography (UPLC), high performance thin layer chromatography (HPTLC), liquid chromatography coupled to tandem mass spectrometry (LC-MS) and capillary electrophoresis (CE), (c) other methods such as spectrophotometry, diffuse reflectance near infrared spectroscopy and electrochemical methods.

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“…Sample preparation was done using automated solid phase extraction. Oasis MCX extraction cartridge which is a mixed-mode, cation-exchange,reversed-phase sorbent was used [26]. Oasis MCX, WAX, MAX and WCX extraction cartridges have been used for the solid phase extraction of metoprolol and others beta-blockers from urine samples.…”

Section: Solid Phase Extractionmentioning

confidence: 99%

“…These methods include liquid chromatography coupled with mass spectrometry (LC-MS/MS) [7][8][9][10][11][12][13][14][15][16][17], liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) [18,19], gas chromatography coupled with mass spectrometry (GC-MS) [3,[20][21][22], high performance liquid chromatography (HPLC) [23,24], high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) [25,26], high performance liquid chromatography with fluorescence detection (HPLC-FLD) [2,27,28], high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) [29], reversed-phase ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) [30].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

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Arterial hypertension is a complex disease with many serious complications, representing a leading cause of mortality. Selective beta-blockers such as metoprolol and bisoprolol are frequently used in the management of hypertension. Numerous analytical methods have been developed for the determination of these substances in biological fluids, such as liquid chromatography coupled with mass spectrometry, gas chromatography coupled with mass spectrometry, high performance liquid chromatography. Due to the complex composition of biological fluids a biological sample pre-treatment before the use of the method for quantitative determination is required in order to remove proteins and potential interferences. The most commonly used methods for processing biological samples containing metoprolol and bisoprolol were identified through a thorough literature search using PubMed, ScienceDirect, and Willey Journals databases. Articles published between years 2005-2015 were reviewed. Protein precipitation, liquid-liquid extraction and solid phase extraction are the main techniques for the extraction of these drugs from plasma, serum, whole blood and urine samples. In addition, numerous other techniques have been developed for the preparation of biological samples, such as dispersive liquid-liquid microextraction, carrier-mediated liquid phase microextraction, hollow fiber-protected liquid phase microextraction, on-line molecularly imprinted solid phase extraction. The analysis of metoprolol and bisoprolol in human plasma, urine and other biological fluids provides important information in clinical and toxicological trials, thus requiring the application of appropriate extraction techniques for the detection of these antihypertensive substances at nanogram and picogram levels.

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Simultaneous determination of bisoprolol and hydrochlorothiazide in human plasma by HPLC coupled with tandem mass spectrometry

Cited by 44 publications

References 24 publications

The effect of pH and ionic strength of dissolution media on in-vitro release of two model drugs of different solubilities from HPMC matrices

The effect of pH and ionic strength of dissolution media on in-vitro release of two model drugs of different solubilities from HPMC matrices

Analytical Techniques for Determination of Hydrochlorothiazide and its Combinations: A Review

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

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