Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry

Kim, Hyung Min; Park, Ju-Hwan; Long, Nguyen Phuoc; Kim, Dae‐Duk; Kwon, Sung Won

doi:10.1016/j.jfda.2019.06.001

Cited by 32 publications

(25 citation statements)

References 25 publications

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“…These data do not correlate quite with the work [16] where the ratio ACN:MeOH (1:2, v/v) was selected for atenolol extraction from DBS. From other hand, the difference in recovery is negligible: 1201 ± 40 (signal area) for 60% of ACN and 1112 ± 71 (signal area) for 40% of ACN.…”

Section: Resultscontrasting

confidence: 55%

“…Protein precipitation or liquid-liquid extraction are a two-step extraction methods where in first step the blood or plasma spot extracted into the aqueous phase and then proteins from the DBSs precipitated by acetonitrile or target substance extracted in water-immiscible organic solvent, respectively. Organic extraction is a one-step extraction method that simply adds organic solvent directly to DPS samples [16]. In this case, proteins and red blood cells remain inside the stain, and the target substance is extracted into a solvent.…”

Section: Resultsmentioning

confidence: 99%

“…In several works the DBS assay was used for atenolol determination [2], but there are no studies for atenolol quantification in DPS. Moreover only in one recent research authors discuss the atenolol extraction from DBS [16]. The aim of this study was to find the optimized parameters of atenolol extraction from DPS.…”

Section: Equipment and Hplc Conditionsmentioning

confidence: 99%

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Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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Aims: Atenolol is one of the β-blockers widely used for the treatment of hypertension and other cardiovascular diseases. To simplify the methods for determining of drugs concentrations in blood and plasma the dried spots assays (dried blood spots or dried plasma spots) could be used. In this case high sensitive detector like mass-spectrometer is required as well as high level of drug recovery from dried spot. In this study the extraction of atenolol from dried plasma spots (DPS) was studied to offer the optimum parameters of extraction method. Study Design: Short research articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between January and October 2019. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: % of organic solvent, presence or absence of 0.1% formic acid, Short Research Articletime, volume and temperature of extraction were investigated to find the best combination for atenolol recovery from DPS for further LC Results: Results showed that the solvent composition and temperature has main influence on the extraction. The effect of extraction time and volume of solvent have no significant influence on atenolol recovery. Pure acetonitrile is the worst solvent for atenolol extraction from DPS. The solvents: MeOH:H 2 O (60:40, v:v), MeOH:0.1% FA in H v:v) or ACN:MeOH (60:40, v:v) provide the best recovery of atenolol temperature is 40°C, time of extraction is 15 Conclusion: Several solvents acceptable for LC from DPS can be used for routine extraction of atenolol.

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Section: Resultscontrasting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

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Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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“…Because DBS is now being increasingly recognized as a great option to collect blood samples (Dittakavi, Jat, & Mullangi, 2019; Kim, Park, Long, Kim, & Kwon, 2019; Kumar Saini et al, 2019; Linder et al, 2019; Sulochana, Daram, Srinivas, & Mullangi, 2019), we were interested in developing and implementing a novel strategy for carrying out ISR analysis using quarter‐, half‐ and full‐DBS discs. As depicted in the Figure 1, this framework of dividing DBS cards (into two equal pieces or four equal pieces, as the case may be) would render itself as a useful approach for preparing aliquots of study samples collected on DBS cards for ISR analysis.…”

Section: Discussionmentioning

confidence: 99%

Novel methodology to perform incurred sample reanalysis on dried blood spot cards: Experimental data using darolutamide and filgotinib

Kiran

Dixit

Gabani

et al. 2020

Biomedical Chromatography

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Different options on performing incurred sample reanalysis (ISR) on dried blood spot (DBS) cards were investigated using drugs belonging to various therapeutic areas: (a) darolutamide (to treat prostate cancer) and (b) filgotinib (to treat rheumatoid arthritis). The proposed novel methodology included the generation of half-DBS and quarter-DBS discs after initial blood collection using the full-DBS discs. Accordingly, blood collection via DBS was performed in male BALB/c mice following intravenous and oral dosing of darolutamide; in male Sprague Dawley rats following intravenous and oral dosing of filgotinib. The ISR data generated from the full-DBS disc, half-DBS disc and quarter-DBS disc were compared for the assessment of the proposed methodology. Quantification of darolutamide and filgotinib was accomplished using liquid chromatography-electrospray ionization/tandem mass spectrometry methods. Darolutamide and filgotinib ISR samples, which were collected and prepared using full-, half-and quarter-DBS discs, met the acceptance criteria for ISR analysis. In conclusion, this is the first report showing a viable tool for the performance of ISR on DBS cards. The use of quarter-or half-DBS discs would aid in not only ISR but also in long-term storage experiments of analytes because it would avoid the need for additional blood sampling in patients.

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“…This method has various advantages, such as minimal invasiveness, minimal risk of infection with infectious pathogens, and ease of storage and transportation. Therefore, this method together with mass spectrometry is convenient to use for preclinical or clinical pharmacokinetic studies [12][13][14]. However, the difference in the hematocrit values in human blood can negatively affect the measured concentration of drugs.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Extraction Procedure of Apixaban from Dried Rat Plasma Spots

Aksenova

Koval

Chernonosov

2020

JPRI

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Aims: Apixaban is an anticoagulant used to treat and prevent blood clots, as well as to prevent stroke in people with atrial fibrillation. The dried spot analyses, including dried blood spots and dried plasma spots, are used to simplify techniques for determining drug concentrations in blood and plasma. In this case, equipment with highly sensitive detector is required, for example, mass spectrometer, as well as a high level of drug extraction from the dried spot. In this work, apixaban extraction from dried plasma spots (DPS) was studied in order to determine the optimal parameters of the extraction method. Study Design: Short Research Articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between September 2019 and February 2020. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: percentage of organic solvent, presence or absence of 0.1% formic acid (FA), time, volume and temperature of extraction were investigated to find the best combination for recovery of apixaban from DPS for further LC-MS analysis. Results: The results showed that the main influence on the extraction is the composition of the solvent, volume of solvent, as well as temperature and time of extraction. Pure acetonitrile is the worst solvent for extracting apixaban from DPS. Solvents: MeOH:H2O (100:0, v: v), MeOH: 0.1% FA in H2O (80:20, v:v), ACN: 0.1% FA in H2O (90:10, v:v) or ACN:MeOH (90:10, v:v) provide better recovery of apixaban. The optimum extraction parameters were as follows: 90% acetonitrile concentration, extraction temperature of 40°C, extraction time of 15 min, and solvent volume of 100

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Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry

Cited by 32 publications

References 25 publications

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Novel methodology to perform incurred sample reanalysis on dried blood spot cards: Experimental data using darolutamide and filgotinib

Optimization of the Extraction Procedure of Apixaban from Dried Rat Plasma Spots

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