Simultaneous Determination of Cefepime and Tazobactam in Injectables by Ultra-High Performance Liquid Chromatography Method

Shrestha, Bhupendra; Bhuyan, Nihar Ranjan; Sinha, Barij Nayan

doi:10.5530/phm.2014.1.4

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…It is used to treat urinary tract infections, septicemia, staphylococcal infections, bronchitis, intra-abdominal infections, skin and its structures infections, pneumonia infections, and in febrile neutropenic patients (Glish and Burinsky, 2008;Lemke et al, 2002). CFP stability was estimated using several methods using chromatography (Abd El Aziz Shama et al, 2021;Al Kamaly, 2022;Bjergum et al, 2021;De Borba et al, 2008;Dos Anjos et al, 2022;El-Beltagy et al, 2019;El-Dars et al, 2019;Fage et al, 2021;Isla et al, 2005;Jagadeesh Kumar et al, 2010;Jiang et al, 2010;Jiménez Palacios et al, 2005;Kalyani et al, 2018;Kommana et al, 2014;Liu et al, 2018;Mameli et al, 2019;Moorthy et al, 2020;Nemutlu et al, 2009, Ocaña González et al, 2004Ohmori et al, 2011;Patil et al, 2018;Rehm and Rentsch, 2020;Rodrigues et al, 2016;Seraisso et al, 2022;Shrestha et al, 2014;Siddiqui et al, 2010;Sun et al, 2022;Sundara Raj et al, 2013;Sunitha et al, 2013;Van Vooren and Verstraete, 2021;Zander et al, 2015) but these methods had some disadvantages as complex mobile phase composition, increased matrix effect, decreased sensitivity, and inability to identify degradants using trace analysis in addition to fragmentation (Niessen, 2010;Niessen and Ricardo, 2017), less environmentally friendly, and time-consuming. However, there have been no previous analytical procedures of trace analysis reported on CFP stability studi...…”

Section: Introductionmentioning

confidence: 99%

Stability indicating UPLC-MS/MS method for quantification and identification of cefepime and its degradants in API

Jabeen¹,

Suma²

2023

J App Pharm Sc

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An analytical method was developed and validated for determining stability studies of the drug cefepime (CFP) by Ultra Performance Liquid Chromatography -Tandem Mass Spectrometer (UPLC-MS/MS). In this research, Ultra Performance Liquid Chromatography (UPLC) C18 ethylene hybrid column was used. About 0.2% formic acid in the water and acetonitrile (ACN), in 20:80 v/v ratios, was used as the mobile phase with a 0.15 ml/minute flow rate. In multiple reaction monitoring modes, positive electrospray ionization (ESI) was used. The chromatogram of standard Cefepime (CFP) was found to have a retention time of 0.82 ± 0.02 minute. The degradation of CFP was tested under different stress conditions and the method was validated to meet the International Conference on Harmonization of Technical Requirement for Registration of Pharmaceuticals for Human Use guidelines. The retention time of the forced degraded solutions of CFP was found to be 0.76, 0.83, 0.81, and 0.82 minutes for forced acidic, oxidation, thermal, and neutral conditions, respectively. The MS/MS spectra for CFP at 179.15 and 241.33 ± 0.06 in different stress conditions (except basic) with retention times at 0.90 ± 0.02 and 0.84 ± 0.10 minutes indicate Cefepime degradant 1 (CD1) and Cefepime degradant 2 (CD2), respectively. The MS/MS spectra for CFP basic degradant (CD1) in the basic medium were obtained at an m/z ratio of 179.15. The research findings conclude that CFP was unstable with partial degradations in all conditions, and complete degradation in basic medium.

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Section: Introductionmentioning

confidence: 99%

Stability indicating UPLC-MS/MS method for quantification and identification of cefepime and its degradants in API

Jabeen¹,

Suma²

2023

J App Pharm Sc

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“…Literature review revealed the reporting of different methods for the estimation of the PPC and TZB drugs either alone, together or in combination with other antibiotics. Several methods were reported for the determination of PPC/TZB [15,16] or CFM/TZB [17,18]. However, only few have been reported for the simultaneous determination of the three drugs under study using HPLC-MS detection in biological fluids [19][20][21][22], and one capillary zone electrophoresis (CZE) method [23].…”

Section: Introductionmentioning

confidence: 99%

Design of an Experimental Study for the Simultaneous Determination of Cefepime, Piperacillin and Tazobactam Using Micellar Organic Solvent-Free HPLC

et al. 2022

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Application of Sustainable analytical chemistry concepts has become crucial in order to remove the environmentally harmful impacts originating from the routine use of analytical techniques. Here, a new LC method is developed and its parameters are analyzed, depending on a mixed micellar mobile phase. This was primarily aimed at getting rid of the use of organic solvents in conventional routine analyses. Combinations of tazobactam (TZB) with piperacillin (PPC) or cefepime (CFM) are commonly used as effective antimicrobial therapies, especially for resistant strains. Therefore, the three drugs were separated and quantified using an organic solvent-free mobile phase. The mixed micellar mobile phase was comprised of 15 mM Brij-35 with 38 mM SDS, adjusted to pH 3.5. Separation was performed by HPLC on monolithic RP-C18 column Chromolith® Performance RP-18e (100 mm × 4.6 mm) at a rate of 1 mL per minute of flow in conjunction with a measurement wavelength 210 nm. The method was found valid and applicable in accordance of precision, and accuracy within ranges of 5–100 µg mL−1 for PPC and CFM and of 0.625–12.5 µg mL−1 for TZB. The quality-by-design technique was used to analyze the effect of modifying the mixed micellar ratios on separation efficiency and conclude their behavior. Finally, the suggested approach was assessed applying the green analytical procedure index against the greenest published methodology to show superiority.

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“…Cefepime or its content of N-methyl pyrrolidine were also determined by several methods such as HPLC [12,13], MEKC [14,15], GC [16,17], TLC [18,19], ion chromatography [20,21], spectrophotometry [22,23], spectrofluorimetry [24,25], voltammetry [26], capillary electrophoresis [27,28] and chemilluminescence [29].…”

Section: Introductionmentioning

confidence: 99%

Application of Silver Nanoparticles for the Spectrophotometric Determination of Cefdinir and Cefepime HCl in Pharmaceutical Preparations and Human Urine Samples

El-Alfy¹,

Ismaiel²,

El-Mammli³

et al. 2019

Nano BioMed ENG

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In this work, the application of silver nanoparticles (Ag NPs) for spectrophotometric determination of cefdinir (CFD) and cefepime HCl (CFP) was developed. The method was based on the ability of the alkaline degradation products of the cited drugs to reduce Ag+ ions to silver nanoparticles (Ag-NPs) in the presence of gelatin as a capping agent. The linear concentration ranges were 5.0-40 and 10-100 µg/mL for cefdinir and cefepime HCl, respectively. The lower detection limits were found to be 0.39 and 1.06 µg/mL for CFD and CFP, respectively, while the quantitation limits were found to be 1.18 and 3.21 µg/mL for CFD and CFP, respectively. The formed nanoparticles were characterized by ultraviolet-visible spectroscopy and transmission electron microscopy. The proposed method was applied successfully to determination of CFD and CFP in pure drugs, pharmaceutical preparations and human urine samples.

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Simultaneous Determination of Cefepime and Tazobactam in Injectables by Ultra-High Performance Liquid Chromatography Method

Cited by 6 publications

References 24 publications

Stability indicating UPLC-MS/MS method for quantification and identification of cefepime and its degradants in API

Stability indicating UPLC-MS/MS method for quantification and identification of cefepime and its degradants in API

Design of an Experimental Study for the Simultaneous Determination of Cefepime, Piperacillin and Tazobactam Using Micellar Organic Solvent-Free HPLC

Application of Silver Nanoparticles for the Spectrophotometric Determination of Cefdinir and Cefepime HCl in Pharmaceutical Preparations and Human Urine Samples

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