Simultaneous determination of ciprofloxacin and tetracycline in biological fluids based on dual-lanthanide sensitised luminescence using dry reagent chemical technology

Rodríguez-Díaz, Rafael Carlos; Aguilar-Caballos, M.P.; Gómez‐Hens, A.

doi:10.1016/s0003-2670(03)00953-x

Cited by 55 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Their emission intensity depends on the substituent attached to the nitrogen atom of the pyridine ring. 4 The largest luminescence intensities of these complexes in solution were observed in the pH range of 5.3-8.5. At lower pH (in acid solutions), the degree of complexation is low.…”

Section: Introductionmentioning

confidence: 96%

Chemiluminescence determination of fluoroquinolones using Fenton system in the presence of terbium(iii) ions

Kaczmarek

Lis

2011

Analyst

View full text Add to dashboard Cite

A simple new chemiluminescent, CL, method is described for the determination of fluoroquinolones such as: ciprofloxacin (CF), norfloxacin (NF), and ofloxacin (OF). This method is based on the measurement of terbium(iii) emission. This emission follows an energy transfer to the uncomplexed terbium(iii) ions from the excited products of fluoroquinolone oxidations. Under optimum conditions, calibration graphs were obtained for 2 × 10(-8)-2 × 10(-6) mol L(-1) of NF; 3 × 10(-8)-2 × 10(-6) mol L(-1) of CF and 4 × 10(-7)-5 × 10(-5) mol L(-1) of OF. The detection limits are 7 × 10(-9) mol L(-1) norfloxacin, 1 × 10(-8) mol L(-1) ciprofloxacin and 1.5 × 10(-7) mol L(-1) ofloxacin. The method was successfully applied to the determination of these drugs in pharmaceutical formulations.

show abstract

Section: Introductionmentioning

confidence: 96%

Chemiluminescence determination of fluoroquinolones using Fenton system in the presence of terbium(iii) ions

Kaczmarek

Lis

2011

Analyst

View full text Add to dashboard Cite

show abstract

“…The carboxylate and the keto oxygen atoms of FQs allow the formation of chelates that show the characteristic luminescence emission of terbium(III) ion at 545 nm via an intramolecular energy transfer process. Several methods, most of them for screening purposes, have been described for the direct determination of FQs using TSL [20][21][22][23][24]. A method for the determination of two FQs based on the TSL plasmonically enhanced by silver nanoparticles has also been reported [25].…”

Section: Introductionmentioning

confidence: 99%

Determination of fluoroquinolone antibiotics by microchip capillary electrophoresis along with time-resolved sensitized luminescence of their terbium(III) complexes

2014

View full text Add to dashboard Cite

We report on the time-resolved detection of the three fluoroquinolone (FQs) antibiotics ciprofloxacin (CIP), enrofloxacin (ENR) and flumequine (FLU). On addition of terbium(III) ions, the terbium(III)-FQs chelates are formed insitu in an on-capillary derivatization reaction of a microfluidic system. The laser-induced terbium(III)-sensitized luminescence of the chelates is measured at excitation/emission wavelengths of 337/545 nm. The analytes can be separated and quantified within less than 4 min. A solid phase extraction step for analyte preconcentration can be included prior to chelation and microchip capillary electrophoresis. The analytical ranges of the calibration graphs for CIP, ENR and FLU are from 10.6 to 60.0, 10.3 to 51.0, and 11.5 to 58.8 ng mL −1 , respectively, and the detection limits are 3.2, 3.1 and 3.6 ng mL −1 , respectively. The precision was established at two concentration levels of each analyte and revealed relative standard deviations in the range from 3.0 to 10.2 %. The method was applied to the analysis of FQ-spiked water samples.

show abstract

“…Sensitivity can also be increased by using surfactants such as Triton X-100, cetyltrimethylammonium chloride (CTACl), or dodecylsulfate sodium salt, because a micellar environment protects Eu III –TC chelates against non-radiative processes. 20,24 Different analytical methods that make use of Eu-sensitized luminescence as the detection system were reported. 25,26…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Doxycycline and Chlortetracycline in Real Samples by Europium-Sensitized Luminescence

2013

View full text Add to dashboard Cite

A simple luminescent methodology for the simultaneous determination of doxycycline and chlortetracycline in pharmaceutical preparations and human urine is proposed. Since the native fluorescence of both analytes is negligible, this method takes advantage of the lanthanide-sensitized luminescence, which provides increased sensitivity. Due to the strong overlapping between the luminescence spectra of both europium complexes, the use of luminescence decay curves to resolve mixtures of the analytes is proposed, particularly as these curves are more selective. A factorial design, with three levels per factor, coupled to a central composite design was selected to obtain a calibration matrix of 13 standards plus one blank sample, which were processed with a partial least-squares analysis. In order to assess the effectiveness of the proposed method, a prediction set of 10 synthetic samples was analyzed, and recovery percentages between 95 and 104% were obtained. Limits of detection, calculated by means of a new criterion, were 3.27 and 1.06 μg L(-1) for doxycycline and chlortetracycline, respectively. The method was tested in three different pharmaceutical preparations containing the analytes, with average recovery percentages of 99.4 ± 1.8 for doxycycline and 100.5 ± 2.1 for chlortetracycline. Moreover, a central composite design was also developed to obtain a calibration matrix that made feasible the simultaneous determination of both tetracyclines in human urine samples. In this case, average recovery percentages were 98.0 ± 4.4 and 97.8 ± 4.6 for doxycycline and chlortetracycline, respectively. No extraction method or prior separation of the analytes was needed.

show abstract

Simultaneous determination of ciprofloxacin and tetracycline in biological fluids based on dual-lanthanide sensitised luminescence using dry reagent chemical technology

Cited by 55 publications

References 27 publications

Chemiluminescence determination of fluoroquinolones using Fenton system in the presence of terbium(iii) ions

Chemiluminescence determination of fluoroquinolones using Fenton system in the presence of terbium(iii) ions

Determination of fluoroquinolone antibiotics by microchip capillary electrophoresis along with time-resolved sensitized luminescence of their terbium(III) complexes

Simultaneous Determination of Doxycycline and Chlortetracycline in Real Samples by Europium-Sensitized Luminescence

Contact Info

Product

Resources

About