Simultaneous determination of clarithromycin, rifampicin and their main metabolites in human plasma by liquid chromatography–tandem mass spectrometry

Velde, Femke de; Alffenaar, Jan‐Willem C.; Wessels, A. Mireille A.; Greijdanus, Ben; Uges, Donald

doi:10.1016/j.jchromb.2009.04.038

Cited by 53 publications

(40 citation statements)

References 26 publications

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“…The limit of detection was 2.0 × 10 −3 ng on column. Previous studies have reported the limit of detection of 4.2 × 10 −4 ng on column 8) and the lower limit of quantitation of 6.6-15 × 10 −3 ng on column 8,9,18) for measurement of CAM in plasma by LC-MS-MS, with linearity of the calibration curve guaranteed until 4 ng on column. 8,9,18) Thus, the performance of the system used in the present study was comparable to LC-MS-MS with respect to sensitivity and measurable range.…”

Section: Columnmentioning

confidence: 98%

“…We believe that the introduction of this HPLC system to medical facilities for analysis of intracellular antibiotic concentrations is more feasible than LC-MS-MS since both the initial and running costs of HPLC are much lower than those of LC-MS-MS and is able to develop the functional analysis suggested by some authors. 18) It is expected that this HPLC system will contribute to the formulation of effective therapeutic regimens for infectious diseases caused by obligate intracellular parasites.…”

Section: Columnmentioning

confidence: 99%

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High Performance Liquid Chromatographic Determination of Clarithromycin in Lymphocytes Using a Post-column with Tris(2,2'-bipyridine) Ruthenium (III) Chemiluminescence Detection

Chiba

Sokura

Ikejima

et al. 2010

JOURNAL OF HEALTH SCIENCE

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The concentration of antibiotics in cells must be determined for effective treatment of infectious diseases caused by obligate intracellular parasites, such as Chlamydia trachomatis and Legionella pneumophila. We confirmed the usefulness of high performance liquid chromatography (HPLC), which is already commonly used in medical facilities, for the measurement of antibiotics in cells. The measurement was carried out using a post-column with tris(2,2 -bipyridine) ruthenium (III) chemiluminescence detection. Clarithromycin (CAM) was used as the model antibiotic. The retention time of CAM on the column was 7.6 ± 0.4 min and the detection limit was 2.0 × 10 −3 ng on column. The linearity of the calibration curve was guaranteed until 2.0 ng on column. This level of performance is comparable to that of liquid chromatography-tandem mass spectrometry. The system was able to monitor changes in the concentration of CAM in lymphocytes. These findings suggest that HPLC, a general-purpose, already widely used detection system, could also contribute to the development of effective individual antibiotic treatment regimens at a wide variety of medical facilities.

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Section: Columnmentioning

confidence: 98%

Section: Columnmentioning

confidence: 99%

High Performance Liquid Chromatographic Determination of Clarithromycin in Lymphocytes Using a Post-column with Tris(2,2'-bipyridine) Ruthenium (III) Chemiluminescence Detection

Chiba

Sokura

Ikejima

et al. 2010

JOURNAL OF HEALTH SCIENCE

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“…Including the preparation of calibration standards and QC samples, it takes approximately 1.5 h to analyse the three drugs in these 12 samples. The maximum capacity is almost 200 samples per 24 h. In the same time, the samples can be analysed for rifampin on a separate LC-MS/MS [7,15] resulting in a processing time similar to the method of Song [6].…”

Section: Clinical Applicationmentioning

confidence: 99%

“…Song et al published a method for the simultaneous determination of all firstline anti-TB drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) [6]. Unfortunately, the polar compounds isoniazid, pyrazinamide and ethambutol elute near the void volume of the system, co-eluting with endogenous compounds resulting in substantial ion suppression [6,7]. As matrix effects may vary within and Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry , Jan-Willem C. Alffenaar between subjects, ion suppression at the time of elution of the compounds of interest should be avoided or overcome using a stable isotope-labelled internal standard [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it was decided to split the patient sample, followed by a subsequent separate analysis of rifampin and a separate one for isoniazid, pyrazinamide and ethambutol together resulting in more reliable results within the same runtime. A previously developed method will be used for the analysis of rifampin [7,15]. The objective of this study was to develop and validate a simple and rapid LC-MS/MS method for the determination of isoniazid, pyrazinamide and ethambutol in human serum.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Sturkenboom¹,

Lijke²,

Jongedijk³

et al. 2015

J Appl Bioanal

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Clinical studies on tuberculosis have shown a correlation between low drug exposure and treatment failure and acquired drug resistance. Objective was to develop a LC-MS/MS method for the quantification of isoniazid, pyrazinamide and ethambutol. Stable isotope-labelled isoniazid-D4 and ethambutol-D4 were used as internal standards. Protein binding of isoniazid, pyrazinamide and ethambutol was investigated and proved low. Therefore, sample preparation using ultrafiltration could be applied, resulting in linear calibration curves in the range of 0.2-8 mg/L for isoniazid and ethambutol and 2-80 mg/L for pyrazinamide. The method was validated according to the guidelines of the FDA. A fast, simple and reliable LC-MS/MS method has been developed for the simultaneous determination of isoniazid, pyrazinamide and ethambutol in human serum for therapeutic drug monitoring and pharmacokinetic studies.

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Strategies in quantitative LC‐MS/MS analysis of unstable small molecules in biological matrices

Li¹,

Zhang²,

Tse³

2010

Biomedical Chromatography

124

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Stability is an important pre-analytical variable for quantitative LC-MS/MS analysis of drug molecules and/or their metabolites in biological matrices. Instability of an analyte in any stage of the bioanalytical process, including sample collection, processing, storage, extraction and LC-MS/MS analysis, can result in under-/over-estimation if an adequate preventive procedure is not in place. In the current review on practical strategies in quantitative LC-MS/MS bioanalysis of unstable small molecules, the common causes of analyte instability were examined. The instability of some analytes is readily predictable because of the presence of certain chemically or biologically labile moieties in the molecules or because the compounds are in an inter-convertible form, e.g. lactone vs hydroxyl carboxylic acid. However, the instability of many other analytes is not readily predictable. Necessary evaluation needs to be conducted to identify the possible instability issues. The current review highlighted some general considerations and specific approaches for developing a robust LC-MS/MS method. In particular, incurred samples should be used as part of routine short-term stability assessment of any unstable analyte during the early stages of method development and validation. This can help unveil any 'hidden' instability issues that, if left unaddressed, could lead to the invalidation of a 'validated' method.

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Simultaneous determination of clarithromycin, rifampicin and their main metabolites in human plasma by liquid chromatography–tandem mass spectrometry

Cited by 53 publications

References 26 publications

High Performance Liquid Chromatographic Determination of Clarithromycin in Lymphocytes Using a Post-column with Tris(2,2'-bipyridine) Ruthenium (III) Chemiluminescence Detection

High Performance Liquid Chromatographic Determination of Clarithromycin in Lymphocytes Using a Post-column with Tris(2,2'-bipyridine) Ruthenium (III) Chemiluminescence Detection

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Strategies in quantitative LC‐MS/MS analysis of unstable small molecules in biological matrices

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