Simultaneous Determination of Co-administrated Deflazacort, Aprepitant and Granisetron in Dosage Forms and Spiked Human Plasma by RP-HPLC/PAD

Abstract

A selective reversed phase high performance liquid chromatography/photodiode array detector (RP-HPLC/PAD) method has been developed for simultaneous determination of the three co-administrated deflazacort, aprepitant and granisetron drugs used with chemotherapy. The three cited drugs have been chromatographed on C18 column using a mobile phase consisting of acetonitrile–0.2% v/v triethylamine (80:20 v/v, pH of 6.6 ± 0.05) with isocratic elution and monitored by photodiode array at 220 nm. International conference on harmonization (ICH) guidelines were followed to validate the developed method. Successful application of the developed method was assessed by the simultaneous determination of the studied drugs in pure forms, dosage forms and plasma samples in the ranges of 0.2–20, 0.4–40 and 0.2–20 μg/mL for deflazacort, aprepitant and granisetron, respectively.

Simple Liquid Chromatography–Tandem Mass Spectrometry Method for Quantitation of Total and Free Aprepitant and Its Active N-Dealkylated Metabolites in Human Plasma

“…The plasma concentration ranges of total aprepitant, ND-AP, and ND-CAP 24 hours after last aprepitant administration were not significantly different from that in previous studies, 9–13 although the study protocols were different. By contrast, the plasma concentration of free aprepitant and ND-AP showed a large interindividual variation.…”

“…The analytical performance data for this method also met the criteria of the US Food and Drug Administration guidance for industry bioanalytical method validation. 15 The plasma concentration ranges of total aprepitant, ND-AP, and ND-CAP 24 hours after last aprepitant administration were not significantly different from that in previous studies, [9][10][11][12][13] although the study protocols were different. By contrast, the plasma concentration of free aprepitant and ND-AP showed a large interindividual variation.…”

“…Several validated methods have been reported for the quantitation of aprepitant in human plasma using liquid chromatographic (LC) techniques coupled to ultraviolet detection 9 or tandem mass spectrometry (MS/MS). [10][11][12] These methods do not use LC or detection conditions for quantitation of aprepitant metabolites in human plasma.…”

A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

“…According to the literature [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], acetonitrile and acidic water media were selected to optimize the mobile phase composition. A series of concentrations, pH values, the ratio of aqueous potassium dihydrogen phosphate solution, and different chromatographic column types were studied to ensure good resolution and appropriate retention time of the five 5-HT 3 receptor antagonists.…”

“…HPLC is commonly used to verify rapid detection systems due to its attractive features, such as high peak efficiencies, great resolution, high sensitivity, good repeatability, and wide application range. HPLC analytic methods for the detection of 5-HT 3 receptor antagonists such as ODT, GNT, TPT, AZT, and RMT are already mature, and most of them use retention data in the qualitative analysis, while the external standard method (ESM) is applicable in quantitative analysis [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. However, some of these methods may take a long time, and the specific drugs to be tested must correspond to reference substances, which cannot meet the requirements of rapid drug detection.…”