Simultaneous Determination of Cortisol, Cortisone, 6 -Hydroxycortisol and 6 -Hydroxycortisone by HPLC

Zheng, Liyun; Luo, Xi; Zhu, Lijun; Xie, Wenzhao; Liu, Shikun; Zhang, Cheng

doi:10.1093/chromsci/bmt034

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…Our previous experiments confirmed that the combined ratio of 6β-hydroxycortisol and 6β-hydroxycortisone to cortisol and cortisone in urinary MR reflects CYP3A4 catalytic ability and not the total activity of CYP3A4 and CYP3A5 isoforms (unpublished data). Another previous study completed by our laboratory showed that the MR was significantly related with the dose-corrected trough concentrations (C 0 /D) of Tac in renal transplant recipients (r= -0.824, P<0.05) [11] .…”

Section: Introductionmentioning

confidence: 76%

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Prediction of tacrolimus metabolism and dosage requirements based on CYP3A4 phenotype and CYP3A5*3 genotype in Chinese renal transplant recipients

et al. 2016

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Aim: To examine how the endogenous CYP3A4 phenotype and CYP3A5*3 genotype of Chinese renal transplant recipients influenced the dose-corrected trough concentration (C 0 /D) and weight-corrected daily dose (D/W) of tacrolimus. Methods: A total of 101 medically stable kidney transplant recipients were enrolled, and their blood and urine samples were gathered. The endogenous CYP3A4 phenotype was assessed by the ratio of 6β-hydroxycortisol and 6β-hydroxycortisone to cortisol and cortisone in urine. CYP3A5*3 genotype was determined using PCR-RELP. Results: In overall renal transplant recipients, a multiple regression analysis including the endogenous CYP3A4 phenotype, CYP3A5*3 genotype and post-operative period accounted for 60.1% of the variability in C 0 /D ratio; a regression equation consisting of the endogenous CYP3A4 phenotype, post-operative period, body mass index, CYP3A5*3 genotype, gender, total bilirubin and age explained 61.0% of the variability in D/W ratio. In CYP3A5*3/*3 subjects, a combination of the endogenous CYP3A4 phenotype, postoperative period and age was responsible for 65.3% of the variability in C 0 /D ratio; a predictive equation including the endogenous CYP3A4 phenotype, post-operative period, body mass index, gender and age explained 61.2% of the variability in the D/W ratio. Base on desired target range of tacrolimus trough concentrations, individual daily dosage regimen was calculated, and all the observed daily doses were within the predicted range. Conclusion: This study provides the equations to predict tacrolimus metabolism and dosage requirements based on the endogenous CYP3A4 phenotype, CYP3A5*3 genotype and other non-genetic variables.

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Section: Introductionmentioning

confidence: 76%

“…The 6β-OHF, 6β-OHE, F and E in urine were measured using the HPLC-UV method developed in our laboratory [11] . The lower limit of quantification was 5 μg/L for all of the compounds in the urine.…”

Section: Analytical Methods 6β-ohf 6β-ohe F and E In Urinementioning

confidence: 99%

Prediction of tacrolimus metabolism and dosage requirements based on CYP3A4 phenotype and CYP3A5*3 genotype in Chinese renal transplant recipients

et al. 2016

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“…6β‐Hydroxycortisol, 6β‐hydroxycortisone, F, and E in urine were measured using the HPLC–UV method developed in our laboratory . The lower limit of quantitation (LLOQ) was 5 μg L −1 for all compounds.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of 6β-Hydroxycortisol and 6β-Hydroxycortisone as Biomarkers for Cytochrome P450 3A Activity: Insight into Their Predictive Value for Estimating Oral Immunosuppressant Metabolism

Luo

Zheng

Cai

et al. 2015

Journal of Pharmaceutical Sciences

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“…Urinary 6β‐OHF and cortisol concentrations have been measured using immunochemical, high‐performance liquid chromatography (HPLC), and liquid chromatography/tandem mass spectrometry (LC‐MS/MS) methods . We previously developed a reliable capillary gas chromatography/MS method for determining both 6α‐OHF and 6β‐OHF concentrations in urine using stable isotope‐labeled analogs as internal standards .…”

Section: Introductionmentioning

confidence: 99%

Sensitive and simultaneous quantitation of 6β‐hydroxycortisol and cortisol in human plasma by LC‐MS/MS coupled with stable isotope dilution method

et al. 2018

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CYP3A phenotyping provides a means for personalized drug therapy. We focused our attention on the plasma 6β-hydroxycortisol (6β-OHF) to cortisol ratio as an index for CYP3A phenotyping. In the present study, we developed a sensitive and reliable method for the simultaneous determination of 6β-OHF and cortisol in human plasma using high-performance liquid chromatography/tandem mass spectrometry together with picolinylester derivatization or nonderivatization methods and 6β-[9,11,12,12- H ]hydroxycortisol and [1,2,4,19- C ]cortisol as internal standards for in vivo CYP3A phenotyping in humans. The lower limits of quantification were 38.513 pg/mL for 6β-OHF and 38.100 pg/mL for cortisol. The relative error and relative standard deviation of the lower limits of quantification were <5% for both methods. The intra-day and inter-day assay reproducibilities of the determined 6β-OHF and cortisol concentrations were consistent with the actual amounts added as relative errors and relative standard deviations for both methods, which were <5.4% and <3.9%, respectively. Both methods were applied for the quantification of plasma 6β-OHF and cortisol concentrations in healthy subjects taking oral contraceptives. The absolute concentrations and time course of 6β-OHF and cortisol were found to be consistent when measured using the 2 methods. The ratio as an index for in vivo CYP3A activity decreased after 21 days of taking oral contraceptives for both methods. To the best of our knowledge, this is the first study reporting the detailed investigation of accuracy and precision in the simultaneous measurement of 6β-OHF and cortisol in human plasma using liquid chromatography/tandem mass spectrometry coupled with stable isotope dilution method, which can be applied to CYP3A phenotyping.

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Simultaneous Determination of Cortisol, Cortisone, 6 -Hydroxycortisol and 6 -Hydroxycortisone by HPLC

Cited by 11 publications

References 9 publications

Prediction of tacrolimus metabolism and dosage requirements based on CYP3A4 phenotype and CYP3A5*3 genotype in Chinese renal transplant recipients

Prediction of tacrolimus metabolism and dosage requirements based on CYP3A4 phenotype and CYP3A5*3 genotype in Chinese renal transplant recipients

Evaluation of 6β-Hydroxycortisol and 6β-Hydroxycortisone as Biomarkers for Cytochrome P450 3A Activity: Insight into Their Predictive Value for Estimating Oral Immunosuppressant Metabolism

Sensitive and simultaneous quantitation of 6β‐hydroxycortisol and cortisol in human plasma by LC‐MS/MS coupled with stable isotope dilution method

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