“…Mild washing conditions, usually consisting of water, a neutral or slightly acidic buffer, sometimes in the presence of a small percentage of organic solvent (up to 10% methanol or acetonitrile), have been applied [44,[46][47][48][49][50]56,57,60,65,67,[69][70][71][72]. Occasionally, a stronger (additional) wash-step was included: 25 or 30% methanol at neutral pH [50,56,66], 40% methanol at alkaline conditions [48,49] or hexane [46].…”