Simultaneous determination of doxorubicin, daunorubicin, and idarubicin by capillary electrophoresis with laser-induced fluorescence detection

Abstract

The separation and simultaneous determination of doxorubicin, daunorubicin and idarubicin was investigated using capillary electrophoresis with laser-induced fluorescence detection. Because the three anthracycline antibiotics were similar in structure and mass, careful manipulation of the electroosmotic flow and electrophoretic mobilities was required. A buffer consisting of 100 mM borate, adjusted to pH 9.5, containing 30% acetonitrile was found to provide a very efficient and stable electrophoretic system for the analysis of the three anthracyclines. The method was applied to the determination of three anthracyclines in serum samples. Responses were linear in the range of 10-500 ng.mL-1 and the detection limits were lower than 0.9 ng.mL-1.

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

“…Although the focus of this review is on chromatographic techniques, it needs to be mentioned that also capillary electrophoresis with UV [84], amperometric [85] and laser-induced fluorescence detection [86][87][88][89][90][91][92] and other techniques [93,94] have been described. The majority of these methods have been reviewed elsewhere [16,17].…”

Dual-fluorophore quantitative high-throughput screen for inhibitors of BRCT–phosphoprotein interaction

“…Given this fact and the unique challenges of finding actives in protein-protein interaction screens, we chose not to discard actives associated with elevated fluorescence intensity values. Indeed, one of our actives, idamycin (also known as idarubicin), has well-documented fluorescent properties [37] while also being known for its antibacterial and antitumor activity.…”

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

“…To date, a number of publications have been reported of the determination of DXR and its metabolite DXR-ol including capillary electrophoresis (CE) with UV detection [4] or laser-induced fluorescence (LIF) detection [5][6][7], radioimmunoassay [8], high performance liquid chromatography (HPLC) with ultraviolet (UV) detection [9][10], fluorescence detection [11][12][13][14][15][16][17][18][19][20][21], chemiluminescence detection [22], electrochemical detection [23] and mass spectrometric detection [25][26][27][28][29]. However, many of these methods lack the enough sensitivity and selectivity which enable the determination of DXR and DXR-ol at small plasma concentration while the high cost and the need for technical experience limits the use of the others.…”