Simultaneous Determination of Eight Water-Soluble Vitamins in Supplemented Foods by Liquid Chromatography

Zafra-Gómez, A.; Garballo, Antonio; Morales, Juan Carlos; García-Ayuso, L. E.

doi:10.1021/jf060346e

Cited by 106 publications

(47 citation statements)

References 22 publications

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“…Thus, these processes may decrease the recovery of unstable vitamins, such as folic acid. A simple precipitation of proteins with zinc acetate and phosphotungstic acid followed by centrifugation and filtration is sufficient for infant formula and beverages, 31 but is not applicable to supplemented food products like cereals. Furthermore, heavy metals in the precipitation solution interact poorly with LC systems.…”

Section: -17mentioning

confidence: 99%

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS C 30 (4.6 mm × 250 mm, 5 µm particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients (r 2 ) higher than 0.995. The developed method was validated with respect to linearity, intra-and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra-and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

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Section: -17mentioning

confidence: 99%

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…The consumption of contaminated food can cause a decrease in immunological defenses, too [24]. The evaluation of dried apricot is an important issue for consumer safety.…”

Section: Resultsmentioning

confidence: 99%

“…For an average adult (60 kg body weight), the PTDI for copper is 48.3 mg [3,46]. Copper contents in the literature have been reported in the range 0.92-6.49 µg/g the lowest and highest contents of copper ion in different apricot samples from Turkey, respectively [24].…”

Section: Resultsmentioning

confidence: 99%

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Fractionation and Speciation of Cu in Different Processing Apricots

Erdoğan¹,

Erdemoğlu²,

Güçer³

2017

J Nutr Food Sci

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An analytical procedure was proposed to study the operational fraction of Cu in ripe-fresh, sun-dried and sulphited-dried apricots harvested from either treated Bordeaux mixture or unexposed trees. Based on the methods that consists pressurized liquid extraction procedure and solid phase extraction to separate diverse Cu fractions on different sorbents such as Dowex 50Wx8-40 and Amberlite XAD-7, and then flame atomic absorption spectrometry (FAAS) and ion selective electrode were used for off-line determination of Cu concentrations in the effluents obtained.Total copper concentrations in different processing the apricot samples exposed to Bordeaux mixture were 4.58, 4.48 and 4.25 µg/g in ripe fresh, sun-dried, sulphuring-sun drying, respectively. However, Cu concentrations in the organic apricot samples were determined 2.92; 2.97 and 2.25 µg/g. While circa 40-59% of total copper are extractable with water solvent including about 67-68% cationic copper, 25-33% organically bound copper and 26-44% labile in Kabaaşı apricot samples. Besides, while about 27-48% of total copper are extractable with methanol: water solvents containing about 40-23% cationic copper, 60-78% organically bound copper and 56-71% of copper was absorbed in the stomach.It was applied to analytical procedures for Cu speciation in the apricot samples were verified and validated.

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“…10,13,[15][16][17] Ion-pairing reagents improve the chromatographic resolution and peak shape, particularly by reducing peak tailing. With these methods, the mixture of an acetate buffer with 1-octanesulfonate and organic solvents (acetonitrile and methanol) was selected as the mobile phase for the pharmaceutical analysis of thiamine in both United States Pharmacopoeia (USP) 18 and Korean Pharmacopoeia (KP) 19 monographs.…”

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confidence: 99%

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

Suh¹,

Kim²,

Jung³

et al. 2013

Bulletin of the Korean Chemical Society

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A novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs) was employed in the absence of volatile organic solvents or ion-pairing reagents to analyze thiamine, a very polar compound, by reverse phase high performance liquid chromatography (RP-HPLC). Due to its strongly hydrophilic nature, thiamine was eluted near the column dead time (t 0 ) using a mobile phase without adding RTILs or ion-pairing reagents, even if a 100% aqueous mobile phase, which has weak elution power under reverse phase conditions, was used. Thus, 1-ethyl-3-methyl-imidazolium hexafluorophosphate ([EMIM][PF 6 ]), which has the strongest chaotropic effect, was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t 0 . Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine thiamine at the proper retention time. Method validation was performed to assess linearity, intra-and inter-day accuracy and precision, recovery and repeatability; all results were found to be satisfactory. The developed method was also compared to the current official United States Pharmacopoeia (USP) and Korean Pharmacopoeia (KP) methods using an organic mobile phase containing an ionpairing reagent by means of evaluating various chromatographic parameters such as the capacity factor, theoretical plate number, peak asymmetry and tailing factor. The results indicated that the proposed method exhibited better efficiency of thiamine analysis than the official methods, and it was successfully applied to quantify thiamine in pharmaceutical preparations.

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Simultaneous Determination of Eight Water-Soluble Vitamins in Supplemented Foods by Liquid Chromatography

Cited by 106 publications

References 22 publications

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Fractionation and Speciation of Cu in Different Processing Apricots

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

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