Simultaneous determination of Fe(III) and Fe(II) in water solutions and tissue homogenates using desferal and 1,10-phenanthroline

Yegorov, Dmitry Yu.; Kozlov, Andrey V.; Азизова, О. А.; Vladimirov, Yu. A.

doi:10.1016/0891-5849(93)90158-q

Cited by 76 publications

(41 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The powder obtained was mixed at ratio 1:2 (w:v) with 10 mM Tris/HCl buffer, pH 7.4, containing deferoxamine mesylate (final concentration 2 mM). After 5 min incubation, samples were transferred to tubuline syringes and frozen in liquid nitrogen (Yegorov et al 1993;Woodmansee and Imlay 2002). EPR measurements were carried out at 77 K; microwave power, 10 mW; time constant, 82 ms; modulation amplitude, 5 G; centerfield 1,600 G; sweep width, 800 G (Linares et al 2003).…”

Section: Oxidative Stress Parametersmentioning

confidence: 99%

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

Heise

Estevez²,

Puntarulo³

et al. 2007

J Comp Physiol B

View full text Add to dashboard Cite

Acute, short term cooling of North Sea eelpout Zoarces viviparus is associated with a reduction of tissue redox state and activation of hypoxia inducible factor (HIF-1) in the liver. The present study explores the response of HIF-1 to seasonal cold in Zoarces viviparus, and to latitudinal cold by comparing the eurythermal North Sea fish to stenothermal Antarctic eelpout (Pachycara brachycephalum). Hypoxic signalling (HIF-1 DNA binding activity) was studied in liver of summer and winter North Sea eelpout as well as of Antarctic eelpout at habitat temperature of 0°C and after long-term warming to 5°C. Biochemical parameters like tissue iron content, glutathione redox ratio, and oxidative stress indicators were analyzed to see whether the cellular redox state or reactive oxygen species formation and HIF activation in the fish correlate. HIF-1 DNA binding activity was significantly higher at cold temperature, both in the interspecific comparison, polar vs. temperate species, and when comparing winter and summer North Sea eelpout. Compared at the low acclimation temperatures (0°C for the polar and 6°C for the temperate eelpout) the polar fish showed lower levels of lipid peroxidation although the liver microsomal fraction turned out to be more susceptible to lipid radical formation. The level of radical scavenger, glutathione, was twofold higher in polar than in North Sea eelpout and also oxidised to over 50%. Under both conditions of cold exposure, latitudinal cold in the Antarctic and seasonal cold in the North Sea eelpout, the glutathione redox ratio was more oxidised when compared to the warmer condition. However, oxidative damage parameters (protein carbonyls and thiobarbituric acid reactive substances (TBARS) were elevated only during seasonal cold exposure in Z. viviparus. Obviously, Antarctic eelpout are keeping oxidative defence mechanisms high enough to avoid accumulation of oxidative damage products at low habitat temperature. The paper discusses how HIF could be instrumental in cold adaptation in fish.

show abstract

Section: Oxidative Stress Parametersmentioning

confidence: 99%

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

Heise

Estevez²,

Puntarulo³

et al. 2007

J Comp Physiol B

View full text Add to dashboard Cite

show abstract

“…The oxidation of ferrous to ferric in an aqueous medium was assayed by using bathophenanthroline-disulfonic acid, which binds to Fe 2+ to form a red colored complex with maximum absorbance at 530 nm (Ulvik and Romslo, 1979;Yegorov et al, 1993).…”

Section: Assay For Iron Oxidationmentioning

confidence: 99%

Pro- or anti-oxidant manganese: a suggested mechanism for reconciliation

HaMai

Bondy

2004

Neurochemistry International

View full text Add to dashboard Cite

“…Although we consider pyoverdin to be a marker of QS, a drop in production may be due to an indirect effect via pH or iron concentration changes (31). To eliminate the chance of false-positive results, the iron concentration was tested by using 1,10-phenanthroline and desferal (63), and the solution pH was checked throughout the experiment.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa by South Florida Plant Extracts

Adonizio¹,

Kong²,

Mathee

2008

Antimicrob Agents Chemother

351

312

View full text Add to dashboard Cite

Quorum sensing (QS) is a key regulator of virulence and biofilm formation in Pseudomonas aeruginosa and other medically relevant bacteria. Aqueous extracts of six plants, Conocarpus erectus, Chamaesyce hypericifolia, Callistemon viminalis, Bucida buceras, Tetrazygia bicolor, and Quercus virginiana, were examined in this study for their effects on P. aeruginosa virulence factors and the QS system. C. erectus, B. buceras, and C. viminalis caused a significant inhibition of LasA protease, LasB elastase, pyoverdin production, and biofilm formation. Additionally, each plant presented a distinct effect profile on the las and rhl QS genes and their respective signaling molecules, suggesting that different mechanisms are responsible for efficacy. Extracts of all plants caused the inhibition of QS genes and QS-controlled factors, with marginal effects on bacterial growth, suggesting that the quorum-quenching mechanisms are unrelated to static or cidal effects.Pneumonia due to microbial infections is a major cause of morbidity and mortality in immunocompromised patients. Pseudomonas aeruginosa hails as the leading pathogen among patients with cystic fibrosis, diffused panbronchitis, and chronic obstructive pulmonary disease (16,29,37). In addition, P. aeruginosa remains one of the major causes of nosocomial infections (10). The success of this organism is largely due to the production of a myriad of virulence factors (including LasA protease, LasB elastase, pyoverdin, pyocyanin, and alginate) and its ability to form intractable biofilms (38).Expression of many of the virulence factors in P. aeruginosa is controlled by a quorum-sensing (QS) system (59), an intercellular communication scheme in which bacteria are able to detect the population density (via signaling molecules and receptors) and control gene expression accordingly (55). P. aeruginosa elaborates two main sets of QS systems: lasI-lasR and rhlI-rhlR (55). LasI and RhlI are synthetases that manufacture the autoinducer signaling molecules N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) and N-butanoyl-L-homoserine lactone (BHL), respectively. These molecules diffuse out into the environment, and when they reach a putative threshold concentration, they activate the receptors lasR and rhlR. These receptors, in turn, coordinate the regulation of pathogenicity. A third signal, the Pseudomonas quinolone signal, also plays an integral role in the QS system (50). This secondary metabolite of P. aeruginosa is incorporated into the QS hierarchy in times of cell stress (43). This intricate communication system of P. aeruginosa is mirrored in many gramnegative pathogenic bacteria, where it coordinates the regulation of virulence, including motility, biofilm formation, and toxin production (18,19,21,48,54).The misuse and abuse of antibiotics in pharmacotherapy have led to the development of widespread resistance in the target organism. The failure of existing antibiotics to control infection makes it crucial to find alternatives to currently available drugs. Since pathogenici...

show abstract

Simultaneous determination of Fe(III) and Fe(II) in water solutions and tissue homogenates using desferal and 1,10-phenanthroline

Cited by 76 publications

References 26 publications

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

Effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (HIF-1) in zoarcid fish

Pro- or anti-oxidant manganese: a suggested mechanism for reconciliation

Inhibition of Quorum Sensing-Controlled Virulence Factor Production in Pseudomonas aeruginosa by South Florida Plant Extracts

Contact Info

Product

Resources

About