Simultaneous Determination of Felbamate, Primidone, Phenobarbital, Carbamazepine, Two Carbamazepine Metabolites, Phenytoin, and One Phenytoin Metabolite in Human Plasma by High-Performance Liquid Chromatography

Simultaneous determination of lamotrigine, phenobarbitone, carbamazepine and phenytoin in human serum by high-performance liquid chromatography

“…There are several HPLC methods reported for determination of lamotrigine in human serum or plasma [4][5][6][7][8][9] and there are various HPLC methods reported for the simultaneous determination of PHT, PB and CBZ [10][11][12][13]. For research work related to the study of drug interactions, pharmacokinetic studies and routine therapeutic drug monitoring, analytical methods that can reliably and simultaneously measure LTG and other AEDs are highly desirable.…”

Modern Methods for Analysis of Antiepileptic Drugs in the Biological Fluids for Pharmacokinetics, Bioequivalence and Therapeutic Drug Monitoring

“…Many subsequent assays separated the same compounds with the inclusion or removal of one or more additional drugs or metabolites such as ethylpenacemide [12], 5-parahydroxyphenyl-5-phenylhydatoin, N-desmethylmethsuximide [13] and lamotrigine and phenyl-2-theyl-malonamide [15]. Another inclusive assay separated ethosuximide, primidone, carbamazepine, phenytoin, Phenobarbital, carbamazepine metatabolites, phenobabital metabolite and felbamate [16]. All of these assays employ ultraviolet (UV) detection, thereby increasing the risk of metabolite or metrix interferences.…”

“…The primary methods for the analysis of serum CBZ are commercial reagent-based techniques like as FPIA and EMIT [22,23]. However, there are also many GC and HPLC techniques with UV or mass spectrometry (MS) detection available and these have the advantage of simultaneously measuring other AEDs and the active metabolite CBZ-10,11-epoxide [15,16,[24][25][26][27][28]. But most of these assays employ UV detection, thereby increasing the risk of metabolite or matrix interferences.…”

“…However, there are also many GC with various detection [25,89] and HPLC methods have been reported [10,11,80,90]. Treatments for refractory epilepsy with a second drug or a combination of drugs indicate the need for simultaneous HPLC analysis for other AEDs [15,16,24,76,91,92].…”

Determination of phenobarbital in human plasma by a specific liquid chromatography method: application to a bioequivalence study

“…10 Simultaneous determination of phenobarbital and some drugs in human plasma was performed by different methods to support pharmacokinetic and clinical studies. [6][7][8][9][11][12][13][14][15][16][17][18][19][20][21][22] A chiral LC method was developed and validated for the determination of phenobarbital and methylphenobarbital enantiomers in human plasma using a C 18 precolumn and a chiral column for the enantiomeric separation. 23 The RP-LC methods were developed with UV detection and using stir bar-sorptive extraction, 11 liquid-liquid extraction (LLE), 12,13,21 direct injection of plasma with manual column-switching technique, 17 SPE, 22 or protein precipitation.…”