Simultaneous determination of five tetracycline and macrolide antibiotics in feeds using HPCE

Abstract

This work demonstrates the potential of HPCE in the analysis of antibiotics in a complex matrix such as feedstuffs. Using 20 mM citric acid-40 mM Na 2 HPO 4 buffer (pH 2.65), the five antibiotics, tetracycline, oxytetracycline, doxycycline, tilmicosin, and tylosin were successfully separated at 30 kV in a 64.5 cm  75 mm id capillary. Good repeatability, stability, and reliability of the method were supported by o10% CV with mean recoveries of 470%, and the limit of detection of the five analytes was 0.5-1 mg/kg. It was for the first time that a capillary electrophoretic method was employed to simultaneously detect five tetracycline and macrolide antibiotics in animal feeds.

A Confirmatory Method Based on HPLC-MS/MS for the Detection and Quantification of Residue of Tetracyclines in Nonmedicated Feed

“…However, recoveries were low, due to the tendency of tetracyclines to form chelation complexes with different cations. Therefore, initial extraction with McIlvaine/EDTA buffer was employed and gave satisfactory results, as in previously reported methods [7, 21, 28, 29]. Tetracyclines dissolved in the McIlvaine/EDTA solution were then extracted with ethyl acetate, as these two solutions are immiscible, and separation could be easily conducted as the organic phase stays on the top layer.…”

Molecularly imprinted solid-phase extraction for determination of tilmicosin in feed using high performance liquid chromatography

“…Zhang et al determined tilmicosin in feeds by high performance liquid chromatography (HPLC) with ultraviolet detection, where the limit of detection was 2 mg kg −1 [3]. Determination of tilmicosin in feeds by capillary electrophoresis has been described, where the limit of detection was 5 mg kg −1 [4]. Because of the variation in components of feeds, it is difficult to find a suitable cleanup procedure in some cases.…”

Determination of five macrolide antibiotic residues in milk by micellar electrokinetic capillary chromatography with field amplified sample stacking

“…At present, the methods for multi-residue analysis of macrolide antibiotics mainly include high-performance liquid chromatography (HPLC) (Clark, Dowling, & Boison, 2009;De La Huebra, Vincent, Bordin, & Rodriguez, 2005;Edder, Coppex, Cominoli, & Corvi, 2002;Lagan a, 2009;Bogialli, Di Corcia, Lagan a, Mastrantoni, & Sergi, 2007;Dickson, 2014;Dickson, O'Byrne, & Chan, 2012;Granja et al, 2009;Horie, Takegami, Toya, & Nakazawa, 2003;Jacobsen, Halling-Sørensen, Ingerslev, & Hansen, 2004;Thompson, Noot, Calvert, & Pernal, 2005;Wang & Leung, 2009;Yang & Carlson, 2004), and capillary electrophoresis (CE) (Deng, Kang, Li, & Xu, 2007a;Deng, Kang, Li, & Xu, 2007b;Liu, Shi, Liu, & Tian, 2010;Tong, Rao, Zhu, Jiang, & Ding, 2009). Compared to HPLC and LC-MS, CE has the virtue of rapid separation and low reagent consumption.…”