Simultaneous determination of fluoxetine, citalopram, paroxetine, venlafaxine in plasma by high performance liquid chromatography–electrospray ionization mass spectrometry (HPLC–MS/ESI)

He, Jiawei; Zhou, Zhi‐Ling; Huan-de, LI

doi:10.1016/j.jchromb.2005.03.006

Cited by 152 publications

(71 citation statements)

References 24 publications

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“…Attempts with traditional reverse phase columns presented poor peak symmetry and tailing problem. Most of the separation methods in literature overcame these problems by use of buffers in mobile phase [6]. The proposed method was able to selectively separate ESC in a short chromatographic run with the use of buffer mobile phase.…”

Section: Resultsmentioning

confidence: 99%

“…Clonazepam (CLZ) is Benzo diazepine, Anticonvulsant drug, chemically it is 5-(o-chlorophenyl)-1,3-dihydro-7-nitro-2H-1,4-benzodiazepin-2-one. Literature survey reveals several spectroscopic [2][3][4], HPLC [5,6] and HPTLC [7][8][9][10][11][12][13][14][15] methods for estimation of Escitalopram and clonazepam individually as well as combination with other drugs. All the reported HPLC methods used buffer in the mobile phase and longer retention time.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Estimation of Escitalopram and Clonazepam by Rp-HPLC in Pharmaceutical Formulation

Chakole¹,

Charde²,

Bhavsar³

et al. 2012

Int J Phytopharm

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This paper presents a RP-HPLC method for simultaneous estimation of Escitalopram and clonazepam in pharmaceutical formulations. The process was carried out on on a 250 × 4.6 mm, 5µ, C 18 column. The flow rate was 1ml/min and eluent was monitored by absorbance at 248 nm using a mixture of Methanol and Buffer (pH 4.0) in the ratio of 90:10 (v/v). The retention times of Escitalopram and Clonazepam was found to be 3.22 and 4.29 min respectively. Calibration plots were linear in the concentration range of 2.5-80 µg mL -1 and 0.125-4 µg mL -1 for Escitalopram and clonazepam respectively. The total run time is 10 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday and intraday), robustness and LOD/LOQ values and it was successfully employed for the simultaneous estimation of Escitalopram and clonazepam in pharmaceutical tablet formulations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Escitalopram and Clonazepam by Rp-HPLC in Pharmaceutical Formulation

Chakole¹,

Charde²,

Bhavsar³

et al. 2012

Int J Phytopharm

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“…Several methods have been devised for the determination of citalopram in pharmaceutical preparations and biological fluids. These include high performance liquid chromatography (HPLC) with UV detectors (Olesen and Linnet, 1996;Duverneuil et al, 2003;Frahnert et al, 2003), HPLC with fluorescence detectors (Matsui et al, 1995;Rochat et al, 1995;Meng and Gauthier, 2005), HLPC/mass spectrometry (Juan et al, 2005; . Gutteck and Rensch, 2003;Kollroser and Schober, 2003), gas chromatography (Gergov et al, 2000;Sane et al, 2002), electrophoretic methods (Flores et al, 2004;Buzinkaiova and Polonsky, 2000;Bjorhovde et al, 2003) and spectrophotometric (Asad, 2006;Asad et al, 2008;Badiadka and Kunnummel, 2010) methods.…”

Section: Introductionmentioning

confidence: 99%

“…1b. The literature study reveals that several UV spectrometric and chromatographic methods available for dapoxetine in combined tablet formulation (Kim et al, 2013;Abirami et al, 2012;Giri et al, 2012;Banik 2014;Kolsure and Hiremath, 2015;Mehta et al, 2011;Nataraj et al, 2011;Rohith and Ananda, 2012).…”

Section: Introductionmentioning

confidence: 99%

Application of Methyl Orange in the Spectrophotometric Determination of Citalopram and Dapotextine in Pharmaceutical Formulations

El‐Didamony¹,

Abo-Elsoad²

2016

J App Pharm Sci

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A simple, rapid and sensitive spectrophotometric method has been proposed for the assay of citalopram (CIT) and dapotextine (DPT) in bulk and pharmaceutical formulations. The method based on the reaction of the selected drugs with methyl orange (MO) in buffered aqueous solution at pH = 5.0 and pH = 3.0 for CIT and DPT, respectively. The formed yellow ion-pair complexes were extracted with dichloromethane and their absorbance was measured at 422 nm. The best conditions of the reaction were studied and optimized. The extracts are intensely colored and very stable at room temperature. The calibration graphs were linear over the concentration range of 1.2 -4.8 g/ml for CIT and 0.4 -4.0 g/ml for DPT. The drug-dye stoichiometric ratio as determined by the Job's method was found to be 1:1. The proposed method was successfully applied for determination of the drugs in tablets with good accuracy and precision. Excipients used as additive in commercial formulations did not interfere in the analysis. The proposed method can be recommended for quality control and routine analysis where time, cost effectiveness and high specificity of analytical technique are of great importance.

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“…5) HPLC-electrospray ionization mass spectrometry (HPLC-MS/ESI) 6) and HPLC-positive ion electrospray mass spectrometry method has been reported for the quantification of citalopram in human plasma. 7) Officially assay of citalopram hydrobromide is not described in pharmacopoeias.…”

mentioning

confidence: 99%

Development and Application of Spectrophotometric Methods for the Determination of Citalopram Hydrobromide in Dosage Forms

Raza

2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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Citalopram is a highly selective serotonin (5-hydroxytryptamine, 5-HT) reuptake inhibitor (SSRI) with minimal effects on the neuronal reuptake of norepinephrine and dopamine. It is a bicyclic phthalane derivative (racemic), which is not related to the tricyclic or tetracyclic antidepressants. It is used in the treatment of major depressive disorder. The hydrobromide salt of it is administered orally. Chemically, it is (Ϯ)-1-(3-dimethylaminopropyl)-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-5-carbonitrile, hydrobromide. 1)A very few analytical methods appeared in the literature for the determination of citalopram hydrobromide in tablets are generally based HPLC with UV detection 2) HPLC with fluorescence detection, 3) capillary zone electrophoretic method 4) densitometric HPTLC, and videodensitometric HPTLC methods. 5) HPLC-electrospray ionization mass spectrometry (HPLC-MS/ESI) 6) and HPLC-positive ion electrospray mass spectrometry method has been reported for the quantification of citalopram in human plasma.7) Officially assay of citalopram hydrobromide is not described in pharmacopoeias. In the present work, an attempt was made to provide a simple, accurate and low cost spectrophotometric method for the quantitative determination of citalopram hydrobromide in pharmaceutical preparations without the interferences of other constituent in the formulations. ExperimentalApparatus A Hitachi U 1100, UV/Vis spectrophotometer (Japan) with silica glass cell of 1 cm thickness was used. Officially calibrated Pyrex glassware was used throughout this study.Reagents and Standards Citalopram hydrobromide was supplied by Bio Fine pharmaceuticals (Pvt.) Ltd. Multan, Pakistan. Commercial dosage forms of citalopram hydrobromide were purchased from a local market. All reagents and solvents used were of Analytical Reagent Grade. 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) (Fluka, Switzerland) 2.0 mg ml Ϫ1 solution was prepared in 1,4-dioxan. The standard solutions (1 mg ml Ϫ1) of citalopram hydrobromide were prepared in methanol for method A and citalopram hydrobromide base in chloroform for method B. An aqueous solution of sodium carbonate (0.5 M, Merck, Germany) was prepared in doubly distilled water.Recommended Procedures. Method A Different aliquots of standard solution of citalopram hydrobromide (5-40 mg ml Ϫ1 ) were prepared in methanol. Absorbance of these solutions was noted at 240 nm against a reagent blank.Procedure for the Assay of Citalopram Hydrobromide in Pharmaceutical Formulations Twenty tablets were accurately weighed and powdered. A portion equivalent to 100 mg of citalopram hydrobromide was stirred with 30 ml methanol and the volume was made up to 50 ml in volumetric flask. The solution was filtered with Whatman filter paper No. 1. Then take aliquot of the filtrate made up to 100 ml volume with methanol in volumetric flask. The absorbance was measured at 240 nm against reagent blank.Method B. Preparation of Citalopram Base Solution A citalopram base solution was prepared by transferring 100 ml of 0.5% cital...

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Simultaneous determination of fluoxetine, citalopram, paroxetine, venlafaxine in plasma by high performance liquid chromatography–electrospray ionization mass spectrometry (HPLC–MS/ESI)

Cited by 152 publications

References 24 publications

Simultaneous Estimation of Escitalopram and Clonazepam by Rp-HPLC in Pharmaceutical Formulation

Simultaneous Estimation of Escitalopram and Clonazepam by Rp-HPLC in Pharmaceutical Formulation

Application of Methyl Orange in the Spectrophotometric Determination of Citalopram and Dapotextine in Pharmaceutical Formulations

Development and Application of Spectrophotometric Methods for the Determination of Citalopram Hydrobromide in Dosage Forms

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