Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography–electrospray tandem mass spectrometry

The endogenous plant hormones and ratios regulate sugar and dry matter accumulation in Jerusalem artichoke in salt-soil

“…Identification and quantification of endogenous hormones (IAA, GA 3 , and ABA) was done according to Hou et al (2008) and ZT was done according to Ge et al (2004) and Muller and Hilgenberg (1986) with some modifications. A 1.00 g sample was ground to fine powder in the presence of liquid nitrogen, then extracted at 4°C for 12 h with 20 mL of 80% (v/v) methanol solution and sealed using a preservative film.…”

“…The aqueous residue was adjusted to 20 mL with water before passing through a preconditioned C18 SPE column (Sep-Pak waters USA; 500 mg, 6 mL). Both cartridges were washed with 1 mL of 20% v/v methanol containing 0.1% v/v formic acid, and the retained phytohormones were eluted with 1 mL of 80% v/v methanol (Hou et al, 2008). Prior to high performance liquid chromatography analysis, this reconstituted eluate was filtered using a 0.45-μm microfiber filter (Ge et al, 2004).…”

Balance between salt stress and endogenous hormones influence dry matter accumulation in Jerusalem artichoke

“…Identification and quantification of endogenous hormones (IAA, GA 3 and ABA) was done according to Hou et al (2008). A 1.00-g sample was ground to fine powder in the presence of liquid nitrogen, then extracted at 4°C for 12 h with 20 mL of 80% v/v methanol solution and sealed using a preservative film.…”

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

“…Liquid chromatography (LC) coupled to electrospray ionization (ESI) and tandem mass spectrometry (MS/MS) is a selective and sensitive tool for the trace analysis of multiple compounds in various matrices. Methods have been developed using LC-MS/MS to quantify melatonin [13], serotonin [13][14][15], HIAA [16,17], IAA [13,[18][19][20], and 3-IS [21]. Amirkhani et al [22] described a method to determine Trp, KYN, and KA in human plasma by capillary LC-MS/MS.…”