Simultaneous determination of ginsenosides inPanax ginseng with different growth ages using high-performance liquid chromatography–mass spectrometry

Abstract

The contents of ginsenosides in Panax ginseng not only vary in different parts of the root, but also exhibit yearly variation. In this study, an HPLC-MS method was established in order to simultaneously analyse ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rf, Rg1 and Rg2. The concentration of ginsenosides in the tap root and root fibre were compared and the yearly variations of nine ginsenosides elucidated. The results indicate that the total content of ginsenosides in the main root and the root fibre both attain a maximum level in the fourth year of growth, although the amount in the former is much higher than in the latter. The variation in the content of ginsenosides during a 2-6 year period suggests that cultivated P. Ginseng can be harvested after the fourth year. The current results will provide useful information for the quality control and good agricultural practice farming of ginseng.

Biosynthesis and biotechnological production of ginsenosides

“…1). Interestingly, during both P. ginseng and P. quinquefolius grow from one to six years, roots or root hairs of older ginseng accumulate a higher content of ginsenosides, such as Rb1, whereas the leaves accumulate ginsenoside maximally during early growth stages (mainly during the first year and the second year) (Li et al, 2012;Shi et al, 2007;Wang et al, 2006) (Fig. 1).…”

Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

“…5 Of these, ginsenoside-Rb1 is the quantitatively most significant component of red ginseng. 6 Ginsenoside-Rg3 reportedly inhibits the transcriptional activities of androgen receptors and downregulates androgen receptors in a prostate cancer cell line. 7 Several murine studies support the hypothesis that ginseng and its ginsenosides may promote hair growth.…”

Sensitive determination of 20(S)-protopanaxadiol in rat plasma using HPLC–APCI-MS: Application of pharmacokinetic study in rats

“…HPLC with UV detection, HPLC-ESI-MS and HPLC-ESI-MS/MS [10,11,[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Rg3, Rh2 and PPD [10]. However, due to insufficient sensitivity the metabolite (PPD) kinetics of Rg3 could not be profiled comprehensively after oral administration of Rg3.…”