Simultaneous determination of glucuronic acid and sulfuric acid conjugated metabolites of daidzein and genistein in human plasma by high-performance liquid chromatography

Metabolism and Disposition of Isoflavone Conjugated Metabolites in Humans after Ingestion of Kinako

“…HPLC-UV-DAD Analysis of Plasma Isoflavone Metabolites. Isoflavone metabolite levels in plasma were determined according to the previously developed HPLC-UV-DAD method (Hosoda et al, 2010a). The metabolites in plasma were extracted by solid-phase extraction (SPE) using an Oasis HLB cartridge (3 cm 3 , 60 mg of packing; Waters, Milford, MA).…”

“…Distilled water was added to dilute the volumes of the collected urine samples 1:4000 within each interval, and the total volumes of the diluted urine samples were adjusted to 1.0 ml. The subsequent sample analyses were performed according to the previously developed SPE method (Hosoda et al, 2010a). Luteolin-3Ј,7-di-O-glucoside (510.0 ng) was added as an internal standard to the diluted urine samples (1.0 ml).…”

“…We previously synthesized 12 conjugated metabolites of Dein and Gein and isolated two compounds from human urine after ingestion of kinako for use as the authentic standard, and we then developed a high-performance liquid chromatography (HPLC)-UV-diodearray detector (DAD) method for simultaneous determination of 16 isoflavone metabolites (Fig. 1) in plasma (Hosoda et al, 2010a). By using this method, we found that D-7-4ЈS, genistein-7-glucuronide-4Ј-sulfate (G-7G-4ЈS), and genistein-4Ј,7-diglucuronide (G-4Ј,7-diG) were the major metabolites of Dein and Gein in humans.…”

An HPLC method for the pharmacokinetic study of daidzein-loaded nanoparticle formulations after injection to rats

“…Recently, a simultaneous method was established to determine daidzein by a solid-phase HLB cartridge [14], and another chromatographic separation method was reported using a mobile phase containing 20 mM sodium acetate, 0.25 mM EDTA, pH 4.3, 4% methanol and 11% acetonitrile in water [15]. Li and Shen [16] detected daidzein by a simple HPLC method without internal standard.…”

Élelmi növények polifenolkészletének vizsgálata tömegspektrometriás módszerekkel

“…Mára már az is ismertté vált, hogy a növényekben a polifenolok főként származékok formájában találhatóak meg, melyek meghatározása sokféleségük miatt igen nehézkes [DE RIJKE et al, 2006]. Napjainkban már az is igazolást nyert, hogy az in vitro körülmények között biológiai hatást eredményező aglikonok konjugált változatai nem azonos mértékben hozzáférhetők az emberi emésztőrendszerben így a hatásosságuk is különböző [DAY et al, 2000;CROZIER et al, 2010;HOSODA et al, 2010].…”