Simultaneous determination of leukotrienes B4 and E4 in whole blood and of leukotriene E4 in urine of rabbit by reversed-phase high-performance liquid chromatography

Celardo, Antonio; Dell’Elba, Giuseppe; Eltantawy, Zeinab Mohamed; Evangelista, Virgilio; Cerletti, Chiara

doi:10.1016/0378-4347(94)00237-1

Cited by 18 publications

(12 citation statements)

References 32 publications

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“…5‐Lipoxygenase metabolites were measured by high performance liquid chromatography (h.p.l.c; Celardo et al ., 1994). Briefly, 1.25 × 10 7 PMN in 500 μl volume were preincubated 2 min with DMSO or trans ‐resveratrol in the presence of 2.5 μg ml −1 cytochalasin B and stimulated for 5 min with 5 μM calcium ionophore A23187.…”

Section: Methodsmentioning

confidence: 99%

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

Rotondo

Rajtar

Manarini

et al. 1998

British J Pharmacology

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215

128

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1 Polymorphonuclear leukocytes (PMN) may contribute to the pathogenesis of acute coronary heart disease (CHD). 2 Epidemiological and laboratory evidence suggests that red wine, by virtue of its polyphenolic constituents, may be more eective than other alcoholic beverages in reducing the risk of CHD mortality. 3 The aim of the present study was to investigate the eects of trans-resveratrol (3,4',5-trihydroxytrans-stilbene), a polyphenol present in most red wines, on functional and biochemical responses of PMN, upon in vitro activation. 4 trans-Resveratrol exerted a strong inhibitory eect on reactive oxygen species produced by PMN stimulated with 1 mM formyl methionyl leucyl phenylalamine (fMLP) (IC 50 1.3+0.13 mM, mean+ s.e.mean), as evaluated by luminol-ampli®ed chemiluminescence. 5 trans-Resveratrol prevented the release of elastase and b-glucuronidase by PMN stimulated with the receptor agonists fMLP (1 mM, IC 50 18.4+1.8 and 31+1.8 mM), and C5a (0.1 mM, IC 50 41.6+3.5 and 42+8.3 mM), and also inhibited elastase and b-glucuronidase secretion (IC 50 37.7+7 and 25.4+2.2 mM) and production of 5-lipoxygenase metabolites leukotriene B 4 (LTB 4 ), 6-trans-LTB 4 and 12-trans-epi-LTB 4 (IC 50 48+7 mM) by PMN stimulated with the calcium ionophore A23187 (5 mM). 6 trans-Resveratrol signi®cantly reduced the expression and activation of the b 2 integrin MAC-1 on PMN surface following stimulation, as revealed by FACS analysis of the binding of an anti-MAC-1 monoclonal antibody (MoAb) and of the CBRM1/5 MoAb, recognizing an activation-dependent epitope on MAC-1. Consistently, PMN homotypic aggregation and formation of mixed cell-conjugates between PMN and thrombin-stimulated ®xed platelets in a dynamic system were also prevented by transresveratrol. 7 These results, indicating that trans-resveratrol interferes with the release of in¯ammatory mediators by activated PMN and down-regulates adhesion-dependent thrombogenic PMN functions, may provide some biological plausibility to the protective eect of red wine consumption against CHD.

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Section: Methodsmentioning

confidence: 99%

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

Rotondo

Rajtar

Manarini

et al. 1998

British J Pharmacology

Self Cite

215

128

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“…For measurement of LTs, blood was incubated with γ‐glutamyl transferase and leucine‐amino peptidase (0.2 U mL −1 each) in order to transform cysteinyl‐LTs to LTE4. The blood was extracted and LTB4 and LTE4 were quantified by HPLC as described [15,16]. Briefly, the extracted residue was reconstituted in the mobile phase (methanol/acetic acid 0.1%, pH 6.5, in deionized water/acetonitrile 60 : 35 : 5 v/v/v) and the analysis performed by isocratic elution at flow rate of 0.5 mL min −1 .…”

Section: Methodsmentioning

confidence: 99%

TxA2-mediated myocardial ischemia as a consequence of an acute lung inflammatory reaction in the rabbit

Evangelista

Dell’Elba

Celardo

et al. 2003

Journal of Thrombosis and Haemostasis

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Summary. Epidemiological studies link acute infection of the respiratory tract to a transient increased risk of acute myocardial infarction. The underlying mechanisms remain unknown. We hypothesized that vasoactive mediators produced by inflammatory cells in the lungs and drained in the coronary circulation may trigger acute myocardial ischemia. To test this hypothesis we used an experimental model in the rabbit. Injection of the bacterial‐derived peptide N‐formyl‐Met‐Leu‐Phe (or N‐formyl‐Methionyl‐Leucyl‐Phenylalanine)(fMLP) in the jugular vein induced massive recruitment of both polymorphonuclear leukocytes (PMN) and platelets in the microcirculation of the lungs, accompanied by rapid and marked increase of leukotriene B4, cysteinyl leukotrienes and thromboxane (Tx) A2 in the aortic blood. In all animals, fMLP evoked ischemic electrocardiographic changes: within the first minute of infusion a profound depression of the ST segment and inversion of the T wave were observed. Mean aortic pressure and heart rate fell to 64.0 ± 6.9 and 83.5 ± 3.1% of the basal levels at 3 and 10 min, respectively. All these alterations were transient. Aspirin, prevented electrocardiographic ischemic changes, reverted bradycardia and hypotension but did not significantly modify either PMN or platelet recruitment nor leukotriene synthesis. Ridogrel, a Tx‐synthase and receptor inhibitor, prevented ECG alterations and bradycardia, but did not prevent and even worsened hypotension; it blocked platelet, but not PMN, sequestration. Pretreatment of animals with intravenous high dose of aspirin prevented ridogrel‐dependent hypotension and platelet inhibition, suggesting that PGI2 contributes to the effects of Tx‐synthase and receptor inhibitor. In hypercholesterolemic rabbits, ECG alterations persisted longer than in normal controls. In summary, our results indicate that acute activation of PMN and platelets in the lungs provokes transient myocardial ischemia, in normal animals that is exacerbated in hypercholesterolemic rabbits. TxA2 appears to be the major mediator of this phenomenon. Moreover the data suggest that a balance between TxA2 and PGI2 plays a pivotal role in platelet activation and recruitment in our model.

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“…Until now, their quantification and studies in biological fluids has remained difficult and generally used immunoassay 7,8 or HPLC with prostaglandins B 2 as internal standards or LTB 4 as external standard. 9,10 However, interference between biological matrix molecules can compromise the interpretation of the results. Measuring techniques must be sensitive and specific, so mass spectrometry associated with gas chromatography (GC-MS) will be a particularly good method as long as adequate internal standards are used.…”

Section: Introductionmentioning

confidence: 98%

[6, 7, 10, 11‐¹³C₄]‐Labelled leukotriene B₄ synthesis: standard for mass spectrometry determination and metabolic studies

Treilhou

Couderc

2001

Labelled Comp Radiopharmac

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Simultaneous determination of leukotrienes B4 and E4 in whole blood and of leukotriene E4 in urine of rabbit by reversed-phase high-performance liquid chromatography

Cited by 18 publications

References 32 publications

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

TxA2-mediated myocardial ischemia as a consequence of an acute lung inflammatory reaction in the rabbit

[6, 7, 10, 11‐¹³C₄]‐Labelled leukotriene B₄ synthesis: standard for mass spectrometry determination and metabolic studies

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Simultaneous determination of leukotrienes B4 and E4 in whole blood and of leukotriene E4 in urine of rabbit by reversed-phase high-performance liquid chromatography

Cited by 18 publications

References 32 publications

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

Effect of trans‐resveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function

TxA2-mediated myocardial ischemia as a consequence of an acute lung inflammatory reaction in the rabbit

[6, 7, 10, 11‐13C4]‐Labelled leukotriene B4 synthesis: standard for mass spectrometry determination and metabolic studies

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[6, 7, 10, 11‐¹³C₄]‐Labelled leukotriene B₄ synthesis: standard for mass spectrometry determination and metabolic studies