Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Hu, Guoxin; Xu, Ren-ai; Dong, Yaoyao; Wang, Yiyan; Yao, Wenwen; Chen, Zhichuan; Chen, Dongxin; Bu, Tiao

doi:10.1016/j.jep.2015.01.020

Cited by 14 publications

(20 citation statements)

References 18 publications

(20 reference statements)

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“…As for alkaloids, they are another type of plant secondary metabolites, usually possess some predominately pharmacological activity, and in some cases, have medicinal or ecological use ( Yang and Stockigt, 2010 ). The major alkaloids found in lotus seeds are bisbenzylisoquinoline alkaloids including liensinine, isoliensinine and neferine, and these alkaloids have various beneficial pharmacological effects, such as anti-depressant, anti-arrhythmia, anti-pulmonaryedema, and anti-HIV properties ( Hu et al, 2015 ).…”

Section: Metabolomics Study On the Lotus Seedsmentioning

confidence: 99%

“…The metabolism and pharmacokinetics of biologically active ingredients in lotus seeds focus on the three major alkaloids, liensinine, isoliensinine, and neferine ( Yu et al, 2013 ; Hu et al, 2015 ). The metabolism of neferine in dog hepatic microsomal incubations was analyzed and characterized by HPLC-ESI-MS/MS.…”

Section: The Application Of Metabolomics In Lotus Seedsmentioning

confidence: 99%

“…The method was linear over the concentration range of 5–1000 ng/mL, and recoveries were more than 75.3%. The main pharmacokinetic parameters after intravenous administration included t 1/2 (h), V d (L/kg), MRT (h), CL (L/h/kg), AUC 0 - t (ng/mL⋅h) and AUC 0 - ∞ (ng/mL⋅h; Hu et al, 2015 ).…”

Section: The Application Of Metabolomics In Lotus Seedsmentioning

confidence: 99%

“…As an important part of suffix omics, metabolomics is powerful in assessing safety and quality of food, assistant breeding, metabolism and pharmacokinetics of lotus seeds derived products, and so forth ( Oms-Oliu et al, 2013 ; Johanningsmeier et al, 2016 ). The wide application of metabolomics in lotus seeds has gained tremendous momentum in recent years ( Itoh et al, 2011 ; Chen et al, 2012a ; Li et al, 2014 ; Zhao et al, 2014 ; Hu et al, 2015 ; Jung et al, 2015 ; Peng et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Current Advances in the Metabolomics Study on Lotus Seeds

2016

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Lotus (Nelumbo nucifera), which is distributed widely throughout Asia, Australia and North America, is an aquatic perennial that has been cultivated for over 2,000 years. It is very stimulating that almost all parts of lotus have been consumed as vegetable as well as food, especially the seeds. Except for the nutritive values of lotus, there has been increasing interest in its potential as functional food due to its rich secondary metabolites, such as flavonoids and alkaloids. Not only have these metabolites greatly contributed to the biological process of lotus seeds, but also have been reported to possess multiple health-promoting effects, including antioxidant, anti-amnesic, anti-inflammatory, and anti-tumor activities. Thus, comprehensive metabolomic profiling of these metabolites is of key importance to help understand their biological activities, and other chemical biology features. In this context, this review will provide an update on the current technological platforms, and workflow associated with metabolomic studies on lotus seeds, as well as insights into the application of metabolomics for the improvement of food safety and quality, assisting breeding, and promotion of the study of metabolism and pharmacokinetics of lotus seeds; meanwhile it will also help explore new perspectives and outline future challenges in this fast-growing research subject.

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Section: Metabolomics Study On the Lotus Seedsmentioning

confidence: 99%

Section: The Application Of Metabolomics In Lotus Seedsmentioning

confidence: 99%

Section: The Application Of Metabolomics In Lotus Seedsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Current Advances in the Metabolomics Study on Lotus Seeds

2016

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“…To ensure the safety and efficacy of liensinine, an accurate, valid, and highly sensitive detection method is needed. Various methods including HPLC [18,19], thinlayer chromatography (TLC) scanning [20], high-speed countercurrent chromatography (HSCCC) [21,22], LC-MS [23][24][25], and HPLC-TOF-MS [26] have been developed for the determination of liensinine alkaloid in plant extracts and biological samples. ese methods are either less sensitive or require large volumes of plasma samples.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Quantification of Liensinine Alkaloid Using a HPLC-MS/MS Method and Its Application in Microvolume Rat Plasma

Wei

Gou

et al. 2021

Journal of Analytical Methods in Chemistry

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Liensinine, an important alkaloid in lotus seed, exhibits multiple functions such as anti-AIDS, anticancer, antidepressant, and antihypertensive properties. In this study, a highly sensitive HPLC-MS/MS method was developed and validated for the quantification of liensinine in microvolume rat plasma as low as 45 μL. Chromatographic separation was carried out using a reverse-phase Gemini-C18 column (100 mm × 3 mm i.d. × 5 μm), and mass selective detection using multiple reaction monitoring was attained using an electrospray ionization source, which operated in the positive mode. Dauricine was used as the internal standard. The precursor-to-product ion transition m/z 611.15 > 206.10 was selected for the detection of liensinine; m/z 625.25 > 206.10 was used for the detection of dauricine. The developed method is linear over the concentration range of 0.05–1000 ng/mL with an excellent coefficient of determination (R2 = 0.991). The recoveries ranged from 92.57% to 95.88% at three quality control levels. Intraday and interday precision and accuracy are less than 12.2% and 6.59%, respectively. The lower limit of quantification (LLOQ) is 0.05 ng/mL. The matrix effect was insignificant and acceptable. The validated method was successfully applied to the pharmacokinetic study of liensinine in rats. This method can be used for in vivo studies as well as quality control of traditional Chinese medicines and herbal tea containing liensinine alkaloid.

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Pharmacokinetics, bioavailability and metabolism of neferine in rat by LC–MS/MS and LC–HRMS

Wang

Shu

et al. 2021

Biomedical Chromatography

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In this study, a simple and sensitive analytical method based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and validated for the determination of neferine in rat plasma. After acetonitrile‐mediated protein precipitation, the samples were separated on an Acquity BEH C18 column (2.1 × 50 mm, 1.7 μm) maintained at 40°C. The mobile phase comprising 0.1% formic acid in water and acetonitrile was delivered at a flow rate of 0.4 ml/min. The mass detection was conducted using multiple reaction monitoring mode with ion transitions at 625.4 > 206.3 and m/z 622.9 > 380.9 for neferine and internal standard, respectively. The assay was demonstrated to be linear over the concentration range of 0.5–1,000 ng/ml, with correlation coefficient >0.999 (r > 0.999). The validated method was further applied to the pharmacokinetic study of neferine in rat plasma. In addition, the metabolism of neferine was investigated using high‐resolution mass spectrometry. A total of six metabolites from rat liver microsomes and plasma were detected and their structures were identified according to their fragment ions. The proposed metabolic pathways of neferine were demethylation, dealkylation, dehydrogenation and glucuronidation.

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Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Cited by 14 publications

References 18 publications

Current Advances in the Metabolomics Study on Lotus Seeds

Current Advances in the Metabolomics Study on Lotus Seeds

Sensitive Quantification of Liensinine Alkaloid Using a HPLC-MS/MS Method and Its Application in Microvolume Rat Plasma

Pharmacokinetics, bioavailability and metabolism of neferine in rat by LC–MS/MS and LC–HRMS

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