Simultaneous determination of linarin, naringenin and formononetin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Bushen Guchi Pill

Abstract

A sensitive and reproducible liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of linarin, naringenin and formononetin in rat plasma after addition of sulfamethoxazole as the internal standard (IS). Separation was carried out on a Diamonsil C18 column (150 × 4.6 mm, 5 µm) with liner gradient elution using methanol (A) and 0.5‰ formic acid aqueous solution (B). Detection was performed on a triple-quadrupole linear ion trap mass spectrometer with the negative ion electrospray ionization in multiple-reaction monitoring (MRM) mode. The MRM transitions were m/z 591.2 → 283.2, 271.0 → 150.9, 266.9 → 252.0 and 252.0 → 155.9 for linarin, naringenin, formononetin and IS, respectively. All analytes showed good linearity within the concentration range (r > 0.9973). The lower limits of quantitation of linarin, naringenin and formononetin were 0.64, 1.07 and 1.04 ng/mL, respectively. Intra-day and inter-day precisions of the investigated components exhibited an RSD within 9.96%, and the accuracy (relative error) ranged from -11.25 to 9.38% at all quality control levels. The developed method was successfully applied to a pharmacokinetic study of linarin, naringenin and formononetin in rats after oral administration of Bushen Guchi Pill.

Bioavailability of tomato polyphenols is enhanced by processing and fat addition: Evidence from a randomized feeding trial

“…, who reported a t max of 2 h after the ingestion of cooked tomato paste in men, and Guo et al. , who obtained a t max of 0.5 h in rats after the administration of Chinese medicine pills, containing naringenin among other compounds.…”

Pharmacokinetic Drug-Drug Interaction and Responsible Mechanism between Memantine and Cimetidine

“…The representative MRM chromatograms of the standard lower limit of quantification (LLoQ) concentrations of memantine or propranolol (IS) and plasma samples obtained from a rat 10 h after the oral administration of memantine (5 mg/kg) were compared with those of blank plasma [ 19 ].…”

“…The stability of memantine and IS in the rat plasma was tested from the low and high QC samples exposed to different conditions [ 19 ]. Short-term stability was calculated by comparing QC samples that were stored for 12 h at 25 °C with the untreated QC samples.…”

Quantification of complanatoside A in rat plasma using LC‐MS/MS and its application to a pharmacokinetic study

“…Six healthy male Sprague–Dawley rats (200–230 g, obtained from the Laboratory Animal Center of Jilin University) were used for the pharmacokinetic study, which was approved by the Animal Care and Use Committee of Jilin University (Changchun, China). The rats were housed and handled as reported previously (Guo et al ., ). The rats were fasted for 12 h before the experiment.…”