Simultaneous determination of major components of Huangqi–Honghua extract in rat plasma using LC–MS/MS and application to a pharmacokinetic study

Abstract

A sensitive and reliable LC-MS/MS method was developed and validated for simultaneous quantification of the major components of Huangqi-Honghua extact in rat plasma, including hydroxysafflor yellow A (HSYA), astragaloside IV (ASIV), calycosin-calycosin-3′-O-sulfate (C-3′-S). After extraction by protein precipitation with acetonitrile and methanol from plasma, the analytes were separated on a Hypersil BDS C 18 column by gradient elution with acetonitrile and 5 mM ammonium acetate. The detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source switched between negative and positive modes.HSYA was monitored in negative ionization mode from 0 to 4.9 min, and ASIV, CAG, calycosin, C-3′-G and C-3′-S were determined in positive ionization mode from 4.9 to 10 min. The lower limits of quantification of the analytes were 6.25 ng/mL for HSYA, 0.781 ng/mL for CAG and 1.56 ng/mL for ASIV and calycosin. The intra-and inter-assay precision (RSD) values were within 13.43%, and accuracy (RE) ranged from −8.75 to 9.92%. The validated method was then applied to the pharmacokinetic study of HSYA, ASIV, CAG, calycosin, C-3′-G and C-3′-S in rat after an oral administration of Huangqi-Honghua extract.

Simultaneous determination and pharmacokinetic study of six components in beagle dog plasma by UPLC–MS/MS after oral administration of Astragalus Membranaceus aqueous extract

“…Recently, another group identified as many as 23 original flavonoids constituents from AM in rats after oral administration using the high performance liquid chromatography equipped with photodiode array‐electrospray ionization‐ion trap‐time of flight multistage mass spectrometry technique (Liu, Li, et al, 2020). Furthermore, Wang et al applied an LC–MS/MS method to the pharmacokinetic study of CG and astragaloside IV in rat plasma after an oral administration of Huangqi–Honghua extract (Wang et al, 2019). Zeng et al developed a UPLC–MS/MS method for formononetin, CG, ononin and astragaloside IV to the pharmacokinetic study of multiple components in rat plasma after intragastric administration of Huangqi decoction (Zeng et al, 2018).…”