Simultaneous determination of metformin and glimepride in pharmaceutical dosage form by reverse‐phase liquid chromatography

Abstract

A simple, rapid, and precise reversed-phase liquid chromatographic method has been developed for the simultaneous determination of metformin in combination with glimepride. Under the developed conditions, good separation of the analytes was achieved in short analysis time. Several parameters affecting the separation of the analytes were studied, including pH and the concentration of SDS. The method is validated and shown to be linear in the range of 25 microg/mL to 150 microg/mL for metformin and 0.1 microg/mL to 0.6 microg/mL for glimepride. The method is applied for the analysis of these analytes in commercially available tablets.

Short term uptake and transport process for metformin in roots of Phragmites australis and Typha latifolia

“…Methods for pharmaceutical determination in water have been described previously (Kolte et al, 2005). In short, filtrated water samples (0.45 lm) were injected into the HPLC (Varian ProStar 210, Germany) by an auto sampler (Varian ProStar 410, Germany) with an injection volume of 25 lL.…”

A Discriminating Dissolution Method for Glimepiride Polymorphs

“…8 There are two polymorphs of this molecule reported in the literature, form I and form II, of which form II has about 3.5-fold higher solubility than that of form I. 9 There is a growing number of studies describing the determination of glimepiride concentration in biological fluids [10][11][12][13][14][15][16][17][18] and pharmaceutical formulations [19][20][21][22][23][24][25][26][27][28][29][30] using a variety of methods. However, there is no dissolution method for glimepiride tablets in the literature.…”

Multi-component plasma quantitation of anti-hyperglycemic pharmaceutical compounds using liquid chromatography–tandem mass spectrometry

“…The reported methods are applicable to analysis of human plasma, equine plasma and urine, and tablet formulations [23][24][25][26][27][28]. Methods include LC procedures for metformin and glipizide with a lower limit of quantitation (LLQ) of 5.0 and 22.5 ng/mL [23], or metformin and glimepride with a LLQ of 22 and 39 ng/mL, respectively [24]. Methods based on LC/MS/MS have been reported for metformin and gliclazide (LLQ range of 7.8-10.0 ng/mL) [25].…”