Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry

Abstract

BackgroundThe new combination of moxifloxacin HCl and cefixime trihydrate is approved for the treatment of lower respiratory tract infections in adults. At initial formulation development and screening stage a fast and reliable method for the dissolution and release testing of moxifloxacin and cefixime were highly desirable. The zero order overlaid UV spectra of moxifloxacin and cefixime showed >90% overlapping. Hence, simple, accurate precise and validated two derivative spectrophotometric methods have been developed for the determination of moxifloxacin and cefixime.MethodsIn the first derivative spectrophotometric method varying concentration of moxifloxacin and cefixime were prepared and scanned in the range of 200 to 400 nm and first derivative spectra were calculated (n = 1). The zero crossing wavelengths 287 nm and 317.9 nm were selected for determination of moxifloxacin and cefixime, respectively. In the second method the first derivative of ratio spectra was calculated and used for the determination of moxifloxacin and cefixime by measuring the peak intensity at 359.3 nm and 269.6 nm respectively.ResultsCalibration graphs were established in the range of 1–16 μg /mL and 1–15 μg /mL for both the drugs by first and ratio first derivative spectroscopic methods respectively with good correlation coefficients. Average accuracy of assay of moxifloxacin and cefixime were found to be 100.68% and 98 93%, respectively. Relative standard deviations of both inter and intraday assays were less than 1.8%. Moreover, recovery of moxifloxacin and cefixime was more than 98.7% and 99.1%, respectively.ConclusionsThe described derivative spectrophotometric methods are simple, rapid, accurate, precise and excellent alternative to sophisticated chromatographic techniques. Hence, the proposed methods can be used for the quality control of the cited drugs and can be extended for routine analysis of the drugs in formulations.

Synthesis of moxifloxacin–Au (III) and Ag (I) metal complexes and their biological activities

“…This table agreed with the elemental analysis of Table 5. The first stage of decomposition corresponds to endothermic dehydration of complexes by the loss of two water molecules and chlorine occurs in the temperature range 145-320 and 140-325°C, respectively (Attimarad et al 2012;Drevensˇek et al 2006). The second decomposition with exothermic peak by the loss of ligand moiety occurs in the temperature at 490 and 492°C, respectively.…”

Ultraviolet Spectroscopy and Its Pharmaceutical Applications- A Brief Review

“…Derivative spectroscopy offers following advantages [22,23]: 1. Resolve overlapping peaks of complex samples such as ternary mixture 2.…”

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation

“…[5][6][7] Hence, diclofenac sodium and moxifloxacin ophthalmic solutions are used simultaneously after the cataract surgery. Several analytical methods such as spectrophotometry, [8][9][10][11][12] RP-HPLC [13][14][15][16][17][18][19][20][21][22][23] and electroanalytical methods [24][25][26] were reported in the literature for the quantification of DCL and MOX alone or with other drugs. However, the simultaneous determination of DCL and MOX has not been reported.…”