Simultaneous determination of multiple compounds of Da-Huang-Xiao-Shi decoction in rat plasma by LC-MS/MS and its application in a pharmacokinetic study

Advances in Chemistry and Bioactivity of Magnoflorine and Magnoflorine-Containing Extracts

“…There are just several reports on the bioavailability of MGN evaluated in animal models. In the study of Tang and collaborators [47], a daily intragastric administration of a complex preparation Xian-Ling-Gu-Bao used in traditional Chinese medicine was studied. Pharmacokinetics of MGN among other 20 components was evaluated in rats upon 1 g/kg/day oral administration.…”

“…Recent studies have demonstrated that MGN shows a number of pharmacological effects including antidiabetic [50], antioxidant [51,52], anti-inflammatory [53], immunomodulatory [54,55], antiallergic, anticancer [47,56], cardiovascular (relaxant) [57], anti-osteoporotic, antibacterial [58], antifungal [25], or antiviral [59] activities. This compound has also been proven to cross the blood-brain barrier and exhibit central action-affecting the regulation of the central nervous system.…”

“…XLGB capsule is an extensively used remedy in Chinese natural medicine for the treatment of osteoporosis [101]. It has been reported that XLGB shows anti-osteoporosis activity in ovariectomized mice [102,103] and rats with oral dosages at 236 mg/kg/day and 270 mg/kg/day, respectively [47]. Moreover, the dose up to 1800 mg/kg/day showed no serious side effects in ovariectomized rats.…”

“…Moreover, the dose up to 1800 mg/kg/day showed no serious side effects in ovariectomized rats. [47,103]. Dose 0.3 g/kg/day used for experiments was based on the body weight conversion to the clinical effective dose of XLGB used for the prevention of postmenopausal osteoporosis.…”

“…Simultaneous assessment of bioactive components in the plasma of rat after oral administration of XLGB at pharmacodynamic doses was efficiently achieved. Among them, MGN was considered as the key-effective substance of XLGB capsule due to their appropriate pharmacokinetic features and high exposure [47].…”