Simultaneous Determination of N-Acetylaspartic Acid, N-Acetylglutamic Acid, and N-Acetylaspartylglutamic Acid in Whole Brain of 3-Mercaptopropionic Acid-Treated Rats Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

Ma, Deshun; Zhang, Jianying; Sugahara, Kazunori; Ageta, Tomiko; Nakayama, Kazunori; Kodama, Hiroyuki

doi:10.1006/abio.1999.4352

Cited by 10 publications

(7 citation statements)

References 20 publications

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“…Glutamate levels were consistent with previous data but showed a wider regional variation [34]. A similar regional variation was found for NAA and NAAG levels, which are also consistent with previous reports [15,18,24]. However, in contrast with superfusion samples the analyte concentrations found in brain tissue samples may be affected by the extraction method used.…”

Section: Applicability Of the Methodology To Brain Tissue Extracts Ansupporting

confidence: 93%

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Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

Shah¹,

Flor²,

Atkins³

et al. 2008

Journal of Chromatography B

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Section: Applicability Of the Methodology To Brain Tissue Extracts Ansupporting

confidence: 93%

“…Over last few years assays for measurement of NAA based on mass spectrometry have been reported by several authors. The limits of detection reported for NAA are acceptable for measurement of the analyte in brain extract [24] and urine [25]. However, these limits of detection are inadequate for determination of NAA and NAAG in superfusates.…”

Section: Introductionmentioning

confidence: 98%

Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

Shah¹,

Flor²,

Atkins³

et al. 2008

Journal of Chromatography B

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“…By comparison, the lower limit of quantification was approximately 17.5 µg/mL for NAA by NMR (10), 70 ng/mL (S/N > 5) for NAA by HPLC (14), 88 ng/mL NAA by HPLC (15), and 175 ng/mL NAA by HPLC-ESI-MS/MS (21). Several methods only report limits of detection, all of which are higher than the lower limit of quantification in the current article (4,12,13,16,17,20).…”

Section: Discussionmentioning

confidence: 77%

N-Acetylglutamate and N-Acetylaspartate in Soybeans (Glycine max L.), Maize (Zea maize L.), and Other Foodstuffs

Hession

Esrey

Croes

et al. 2008

J. Agric. Food Chem.

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N-Acetylglutamate (NAG) and N-acetylaspartate (NAA) are amino acid derivatives with reported activities in a number of biological processes. However, there is no published information on the presence of either substance in foodstuffs. We developed a method for extracting and quantifying NAG and NAA from soybean seeds and maize grain using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The lower limit of quantification for both NAG and NAA was 1 ng/mL. The method was then utilized to quantify NAG and NAA in other foodstuffs (fruits, vegetables, meats, grains, milk, coffee, tea, cocoa, and others). Both NAG and NAA were present in all of the materials analyzed. The highest concentration of NAG was found in cocoa powder. The highest concentration of NAA was found in roasted coffee beans. Both NAG and NAA were found at quantifiable concentrations in all foods tested indicating that these two acetylated amino acids are common components of the human diet.

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“…Its efficacy was confirmed by Engel et al [ 27 ], who showed a reduction of inhibitory postsynaptic currents in CA3 pyramidal cells in slice cultures as the effect of 3-MPA administration. 3-MPA action begins as soon as 3 min and is reversed after 30–60 min [ 28 – 30 ]. In the preliminary studies we established that the level of GABA in tissue samples of barrel cortex was significantly but moderately (by 15%) reduced 20 minutes after intracortical 3-MPA injection.…”

Section: Discussionmentioning

confidence: 99%

Learning-Dependent Plasticity of the Barrel Cortex Is Impaired by Restricting GABA-Ergic Transmission

et al. 2015

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Experience-induced plastic changes in the cerebral cortex are accompanied by alterations in excitatory and inhibitory transmission. Increased excitatory drive, necessary for plasticity, precedes the occurrence of plastic change, while decreased inhibitory signaling often facilitates plasticity. However, an increase of inhibitory interactions was noted in some instances of experience-dependent changes. We previously reported an increase in the number of inhibitory markers in the barrel cortex of mice after fear conditioning engaging vibrissae, observed concurrently with enlargement of the cortical representational area of the row of vibrissae receiving conditioned stimulus (CS). We also observed that an increase of GABA level accompanied the conditioning. Here, to find whether unaltered GABAergic signaling is necessary for learning-dependent rewiring in the murine barrel cortex, we locally decreased GABA production in the barrel cortex or reduced transmission through GABAA receptors (GABAARs) at the time of the conditioning. Injections of 3-mercaptopropionic acid (3-MPA), an inhibitor of glutamic acid decarboxylase (GAD), into the barrel cortex prevented learning-induced enlargement of the conditioned vibrissae representation. A similar effect was observed after injection of gabazine, an antagonist of GABAARs. At the behavioral level, consistent conditioned response (cessation of head movements in response to CS) was impaired. These results show that appropriate functioning of the GABAergic system is required for both manifestation of functional cortical representation plasticity and for the development of a conditioned response.

show abstract

Simultaneous Determination of N-Acetylaspartic Acid, N-Acetylglutamic Acid, and N-Acetylaspartylglutamic Acid in Whole Brain of 3-Mercaptopropionic Acid-Treated Rats Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

Cited by 10 publications

References 20 publications

Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts

N-Acetylglutamate and N-Acetylaspartate in Soybeans (Glycine max L.), Maize (Zea maize L.), and Other Foodstuffs

Learning-Dependent Plasticity of the Barrel Cortex Is Impaired by Restricting GABA-Ergic Transmission

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