Simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study

Abstract

A rapid and high sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of notoginsenoside R1 and ginsenoside Re in rat plasma was developed. The analytes and internal standard, digoxin, were extracted from rat plasma via protein precipitation with methanol and separated on an Phenomenex Gemini C18 column within 2 min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring and positive ion mode. The precursor to product ion transitions monitored for notoginsenoside R1, ginsenoside Re, and internal standard were m/z 955.5→775.5, 969.6→789.1, and 803.6→283.1, respectively. The assay was validated with linear range of 1.9-380 ng/mL for notoginsenoside R1 and 0.5-100 ng/mL for ginsenoside Re. The intra- and interday precisions (RSD%) were within 8.96% for each analyte. The absolute recoveries were greater than 93% for R1 and 96% for Re. Each analyte was stable during all sample storage, preparation, and analytic procedures. The method was successfully applied to a pharmacokinetic study of Xuesaitong dispersible tablets in eight rats.

Analysis of bioactive components and multi‐component pharmacokinetics of saponins from the leaves of Panax notoginseng in rat plasma after oral administration by LC–MS/MS

“…Deglycosylation is the major metabolic pathway for ginsenoside in vivo. Some saponins can be transformed into other saponins, such as NGFc‐GRb3‐GRd , GRc‐GRd , and GRb1‐GRd . Therefore, the process in vivo must be significantly different from notoginsenoside administered alone.…”

“…To date, many studies focused on saponins have been conducted in rat and dog plasma . However, this study emphasizes PPT‐type saponins, which are mainly distributed in PN roots.…”

Certification of standard reference material 3389 ginsenoside calibration solution

“…LC is the most prevalent separation technique for mixtures of ginsenosides because of its speed and selectivity. LC has the additional advantage of compatibility with various detection techniques ______________________________________________________________________________________________________ such as UV absorbance [15][16][17][18][19], evaporative light scattering (ELS) [20], fluorescence (FL) [21], pulsed amperometry (PA) [22], MS [15,23,24], and tandem mass spectrometry (MS/MS) [25][26][27]. UV is the most frequently used detector for simple sample matrixes because of its inexpensive cost and simplicity, but ginsenoside detection is limited to short wavelengths of 200 nm to 205 nm.…”

A validated LC–MS/MS method for the simultaneous determination of 20‐(S)‐protopanaxatriol and its two active metabolites in rat plasma: Application to a pharmacokinetics study

“…Notoginsenosides are found as the major components in Panax notoginseng . They are prescribed for prevention and pretreatment of cardiovascular and cerebrovascular diseases in China for hundreds of years . It has been widely accepted that notoginsenosides are susceptible to metabolism in gut, thereby forming deglycosylated metabolites and aglycones that are more readily absorbed into the body and act as the active substances .…”

“…in China for hundreds of years [1][2][3][4][5]. It has been widely accepted that notoginsenosides are susceptible to metabolism in gut, thereby forming deglycosylated metabolites and aglycones that are more readily absorbed into the body and act as the active substances [6,7].…”