Simultaneous determination of notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and icariin in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry

Deng, Gui-feng; Wang, Dingli; Meng, Ming-xin; Hu, Fan; Yao, Tong-wei

doi:10.1016/j.jchromb.2009.06.003

Cited by 35 publications

(27 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the combinatorial multicomponent therapies of traditional Chinese medicines (TCMs) [11], it is necessary to develop a more comprehensive and global assay to fully evaluate the pharmacokinetics of these active ingredients in TCMs [12][13][14]. Furthermore, a selective and sensitive analytical method for simultaneous quantification and pharmacokinetic studies of HLF in plasma was developed in order to gain a better understanding of the relationship between the pharmacokinetics, pharmacology and bioavailability of the multiple components, which influence the clinical effects of HLF and its rational dosage regiments.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Zhang

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Zhang

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

“…Many kinds of assay system have been developed to identify ginsenosides in ginseng extracts. In the most cases, the arrangements of HPLC are widely investigated [31,32]. HPLC requires pretreatment of the analytical samples (e.g., partition, purification, and concentration) before loading these onto the column.…”

Section: Discussionmentioning

confidence: 99%

Development of New Staining Technology “Eastern Blotting” Using Monoclonal Antibody

Morinaga

Shoyama²

2011

CDDT

View full text Add to dashboard Cite

Ginsenosides contained in Panax species were separated by silica gel TLC blotted to a polyvinylidene difluoride (PVDF) membrane which was dipped in a sodium periodide (NaIO(4)) solution and reacted with protein, preparing a ginsenoside-protein conjugate for binding a ginsenoside on a PVDF membrane. The blotted spots were stained by anti-ginsenoside-Rb1 monoclonal antibody (MAb) and anti-ginsenoside-Rg1MAb, respectively. The newly established immunostaining method, eastern blotting was applied for the determination of ginsenosides possessing protopanaxadiol and/or protopanaxatriol. Double staining of eastern blotting for ginsenosides using anti-ginsenoside-Rb1 MAb and anti-ginsenoside-Rg1 MAb promoted complete identification of ginsenosides in Panax species. This technique has been devised for the chromatographic separation and identification of ginsenosides using polyethersulfone (PES) membrane. It caused an acceptable separation of ginsenoside-Rb1, -Rc and -Rd in various ginseng extracts. Newly developed technique is quite simple and applies for immunoassay system. Ginsenosides separated using a PES membrane were directly treated with a NaIO(4) solution and then reacted with bovine serum albumin (BSA) for making a ginsenoside-protein conjugate. After the blocking, anti-ginsenoside-Rb1 MAb recognized a ginsenoside on a PES membrane and then a sec-ond antibody labeled with enzyme reacted to the first antibody. Finally a substrate was oxidized with the enzyme and de-veloped the staining of ginsenosides. The staining spots of ginsenosides on membrane were quantitatively evaluated by NIH Image indicating at least 62.5 ng of each ginsenoside-Rb1, -Rc and -Rd were detected with clarity. The determination range of three ginsenosides was from 0.125 to 2.0 µg of direct amount on PES membrane.

show abstract

“…Previous studies indicated that icariin possesses many biological actions, such as liver and cardiovascular function improvement, hormone regulation, immunological function modulation, and antitumor activity [1,2]. The recent methods dealing with the analysis of icariin in biological fluids mainly include high-performance liquid chromatography (HPLC) with ultraviolet (UV) or diode array detection (DAD) [2,3], capillary electrophoresis [4], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [5][6][7][8], and ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS) [9].…”

Section: Introductionmentioning

confidence: 99%

Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum

Yang

2012

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min −1 . The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL −1 with the limit of detection of 3.5 ng mL −1 (S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.

show abstract

Simultaneous determination of notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and icariin in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry

Cited by 35 publications

References 13 publications

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Development of New Staining Technology “Eastern Blotting” Using Monoclonal Antibody

Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum

Contact Info

Product

Resources

About

Simultaneous determination of notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and icariin in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry

Cited by 35 publications

References 13 publications

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Simultaneous determination of vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC–ESI-MS/MS

Development of New Staining Technology &#x201C;Eastern Blotting&#x201D; Using Monoclonal Antibody

Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum

Contact Info

Product

Resources

About

Development of New Staining Technology “Eastern Blotting” Using Monoclonal Antibody