Simultaneous determination of nucleosides and their bases in Cordyceps sinensis and its substitutes by matrix solid-phase dispersion extraction and HPLC

Abstract

Nine nucleosides and nucleobases, including uracil, adenine, thymine, uridine, adenosine, thymidine, cytidine, guanosine, and cordycepin in natural Cordyceps sinensis, cultured Cordyceps mycelia, and Cordyceps fruiting bodies were extracted by matrix solid-phase dispersion (MSPD) and determined by HPLC. The experimental conditions for the MSPD extraction were optimized. Florisil was used as dispersant, petroleum ether as washing solvent, and methanol as elution solvent. The Florisil-to-sample ratio was selected to be 4:1 and no additional clean-up sorbent was needed. The calibration curves had good linear relationships (r > 0.9997). The LOD and LOQ were in the range of 12～79 and 41～265 ng/mL, respectively. The intra- and interday precision were lower than 8.3%. The recoveries were between 61.5 and 93.2%. The present method consumed less sample compared with ultrasonic extraction and heating reflux extraction (HRE). The extraction yields obtained by using the present method are much higher than those obtained by UE and comparable to those obtained by HRE.

A metabolomics approach for authentication of Ophiocordyceps sinensis by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

“…However, only 3′-deoxyadenosine (namely, cordycepin), a pharmaceutically active component exhibiting multiple pharmacological actions, was reported to be abundant in CCH. Indeed, cordycepin was originally extracted from C. militaris (Shashidhar et al, 2013), and some studies have shown that large amounts of cordycepin can be found in C. militaris (Guo, Li, Huang, Liang, & Chen, 2006;Wang et al, 2013;Yang, Guan, & Li, 2007), while only trace amounts are present in DCXC, consistent with our current results. Thus, the present result indicated that if the content of cordycepin was abnormally high in the DCXC specimen, adulteration with CCH or C. militaris may have occurred, providing an effective quality control marker.…”

Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis

“…Studies based on the molecular level have confirmed that nucleosides [53], sterols [54], polysaccharides [55] and mannitol [54] were the material basis for pharmacological effects of H. sinensis . At present, there are few reports about metabolic pathways both in H. sinensis and O. sinensis , so it is much more necessary and urgent to investigate metabolic pathways which will provide new information and method to further regulate, control and optimize the fermentation process to obtain H. sinensis mycelium with high quality.…”

Determination of Sudan Dyes in Juice Samples via Solidification of Ionic Liquid in Microwave-Assisted Liquid-Liquid Microextraction Followed by High-Performance Liquid Chromatography

“…The target analytes were identified by comparing their retention times and absorption spectra with those of the authentic standard analytes (Wang et al 2013b). The spectral data for a Mean recovery ± standard deviation, %, n = 5 each chromatographic peak are helpful in the identification of species.…”