Simultaneous determination of organic acids and sugars in apples by gas—liquid chromatography

Simultaneous Determination by Capillary Gas Chromatography of Organic Acids, Sugars, and Sugar Alcohols in Plant Tissue Extracts as Their Trimethylsilyl Derivatives

“…In general, the yield of TMS derivatives (measured as the FID response) increased as the derivatization temperature was increased. This trend was particularly pronounced for the sugars and sugar alcohols, probably due to the abundance of -OH groups in these compounds which require higher temperatures for complete derivatization as reported previously (11)(12)(13). Most compounds were completely derivatized at 100°C as determined by repeated injections into the GC system to check the yield of derivatized compounds.…”

“…A popular approach is based on the original work of Sweeley et al (11) that was subsequently developed by other groups (12,13), where sugars are initially converted to their oximes by reaction with hydroxylamine in pyridine, and then the sugar-oximes and organic acids are converted to their trimethysilyl (TMS) derivatives by reacting with hexamethyldisilazane (HMDS) (11)(12)(13). Advantages of this method are a relatively short reaction time, simple sample handling, and inexpensive reagents.…”

Comparison of GC and HPLC for quantification of organic acids in two jaboticaba (Myrciaria) fruit varieties

“…Although no information was available in the literature on the HPLC of jaboticaba OAs, it has been extensively used for OAs in foods [8][9][10][11][12][13] and fruits [14][15][16][17][18][19][20][21][22][23][24][25] . The procedure described 11 to quantify coffee OAs with an ion exchange column with H 2 S04 (0.009 N) as the eluting solvent appeared to be interesting.…”

Simultaneous quantitation of mono-, di-and trisaccharides by GC-MS of their TMS ether oxime derivatives: II. In honey

“…Honey samples from various Hungarian apiculturists have been indicated by Arabic numbers (1-10): if available, they were also selected by different floral origin, indicated by the same Arabic numbers, such as mixed flower (1)(2)(3)(4)(5)(6)(7)(8), acacia (1)(2)(3)(4)(5)(7)(8)(9), linden (2), sunflower (9), rape (9) and pine (10). Samples were stored in screw capped glass bottles, in a refrigerator, at +5 ~…”

“…Consequently, on the basis of qualitative and quantitative distribution of sugar contents, the authenticity of the natural matrix can also be evaluated. The simultaneous derivatization and gas chromatographic quantitation of more than 40 components, present in a number of fruit and vegetable species, has been reported recently [1][2][3][4][5][6][7][8][9][10]. In spite of these earlier papers, honey proved to be a difficult matrix because its sugar composition is one of the most complex mixtures of natural carbohydrates.…”