1992
DOI: 10.1002/bmc.1130060210
|View full text |Cite
|
Sign up to set email alerts
|

Simultaneous determination of paraquat and diquat in serum using capillary electrophoresis

Abstract: The use of capillary electrophoresis for simultaneous separation and detection of the two bipyridylium herbicides, paraquat and diquat, was investigated. Both herbicides were extracted from fortified sera with disposable ODS-silica cartridges. Separation was carried out using a capillary tube (50 microns i.d., 750 mm) of fused silica containing 10 mM glycine-HCl buffer (pH 3.0), 40 mM NaCl and 20% methanol as the carrier. Paraquat and diquat were completely separated in 10 min at an applied potential of 20 kV.… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
9
0
3

Year Published

1998
1998
2007
2007

Publication Types

Select...
5
2

Relationship

0
7

Authors

Journals

citations
Cited by 44 publications
(12 citation statements)
references
References 22 publications
0
9
0
3
Order By: Relevance
“…A large variety of analytical techniques have been proposed for determination of paraquat, such as molecular absorption spectrophotometry, 7,8 liquid chromatography with UV detection, 9,10 liquid and gas chromatography coupled to mass spectrometry detection, 11,12 gas chromatography with specific detector for nitrogen and phosphorus (NPD), 13 ELISA 14 as well as potentiometric and amperometric sensors. 15,16 More recently, capillary electrophoresis with ultraviolet (CE-UV) 17 or mass spectrometry (CE-MS) 18 detection was proposed. These techniques suffer with the instability of reagents, interferences, high detection limits, extensive sample treatment and high cost per analysis.…”
Section: Introductionmentioning
confidence: 99%
“…A large variety of analytical techniques have been proposed for determination of paraquat, such as molecular absorption spectrophotometry, 7,8 liquid chromatography with UV detection, 9,10 liquid and gas chromatography coupled to mass spectrometry detection, 11,12 gas chromatography with specific detector for nitrogen and phosphorus (NPD), 13 ELISA 14 as well as potentiometric and amperometric sensors. 15,16 More recently, capillary electrophoresis with ultraviolet (CE-UV) 17 or mass spectrometry (CE-MS) 18 detection was proposed. These techniques suffer with the instability of reagents, interferences, high detection limits, extensive sample treatment and high cost per analysis.…”
Section: Introductionmentioning
confidence: 99%
“…Their LOQs (PQ 15 ng/mL, DQ 8 ng/mL with S/N 3/1) were better and the difference observed could be explained by the injection mode used. Concerning the serum samples, Tomita et al (1992) achieved better recoveries from spiked serum (>95%) using a solid-phase extraction and an hydrodynamic injection mode. Their LOQs were 50 ng/mL with S/N 2/1 for both PQ and DQ with the same injection mode.…”
Section: Discussionmentioning
confidence: 94%
“…It is a highly efficient separation technique, which requires small sample volumes (a few nL), and this method seems to be very well adapted to the herbicide determination in biological fluids. Wu and Tsai (1998) have studied PQ and DQ determinations in urine and Tomita et al (1992) that in serum only. A capillary electrophoresis method for simultaneous quantification of PQ and DQ both in serum and urine is described and the results obtained in two letal poisoning cases are discussed.…”
Section: Introductionmentioning
confidence: 98%
“…solid-phase extraction. [9][10][11] In order to shorten the time required to analyze PQ and DQ in serum, we studied a protein precipitation method usually using acetonitrile, methanol, perchloric acid and trichloroacetic acid as chemical reagents.…”
Section: Postcolumn Reduction Conditionsmentioning
confidence: 99%
“…11 However, these methods have a limited sensitivity and/or specificity and require laborious and time-consuming sample preparation procedures such as cation-exchange chromatography, ion-pair liquid-liquid extraction and/or solidphase extraction. 12 HPLC methods are sensitive and specific, but these methods require either a column switching system 8 or time-consuming extraction procedures.…”
Section: Introductionmentioning
confidence: 99%