Simultaneous determination of polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection

Du, Mingrui; Flanigan, V. J.; Ma, Yinfa

doi:10.1002/elps.200405896

Cited by 74 publications

(40 citation statements)

References 39 publications

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“…The precipitates were washed two times with 0.1 M HClO 4 , and all of the supernatants were combined into one tube. The polyamines were derivatized with fluorescein-5-isothiocyanate and quantified by the capillary electrophoresis method as described previously (9).…”

Section: Methodsmentioning

confidence: 99%

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

et al. 2006

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Polyamines such as putrescine, spermidine, and cadaverine are small, polycationic molecules that are required for optimal growth in all cells. The intracellular concentrations of these molecules are maintained by de novo synthesis and transport pathways. The human pathogen Streptococcus pneumoniae possesses a putative polyamine transporter (pot) operon that consists of the four pot-specific genes potABCD. The studies presented here examined the involvement of potD in polyamine transport and in pneumococcal pathogenesis. A potDdeficient mutant was created in the mouse-virulent serotype 3 strain WU2 by insertion duplication mutagenesis. The growth of the WU2⌬potD mutant was identical to that of the wild-type strain WU2 in vitro in rich media. However, WU2⌬potD possessed severely delayed growth compared to wild-type WU2 in the presence of the polyamine biosynthesis inhibitors DFMO (␣-dimethyl-fluroornitithine) and MGBG [methylgloxal-bis (guanyl hydrazone)]. The mutant strain also showed a significant attenuation in virulence within murine models of systemic and pulmonary infection regardless of the inoculation route or location. These data suggest that potD is involved in pneumococcal polyamine transport and is important for pathogenesis within various infection models.

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Section: Methodsmentioning

confidence: 99%

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

et al. 2006

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“…Because of the significance of catecholamines in clinical diagnosis and medical treatment, rapid and sensitive determination of these compounds in biological samples and pharmaceutical formulations is therefore very important. A variety of techniques, such as spectrophotometry, [1,2] fluorimetry [3,4] and electrochemistry, [5,6] have been utilized for the detection of catecholamines. However, some of these methods lack sensitivity and selectivity or require derivation steps or sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of chemiluminescence of the KIO₄–luminol system by gallic acid, acetaldehyde and Mn²⁺: application for the determination of catecholamines

Chen

Lin

2009

Luminescence

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Chemiluminescence (CL) from the oxidation of luminol with potassium periodate in strong alkaline solutions was greatly enhanced by the combined effect of gallic acid, acetaldehyde and Mn(2+). The CL spectra exhibited only one emission band at 425 nm, indicating 3-aminophthalate as the emitting species. Various scavengers for superoxide anion, hydroxyl radical and singlet oxygen quenched the CL emission very efficiently (74-100%), suggesting the possible involvement of these reactive oxygen species (ROS) in the CL reactions. It is postulated that oxidation of gallic acid and acetaldehyde by periodate catalyzed by Mn(2+) generates these ROS, which then react with luminol to enhance the CL emission. We also found that the enhanced CL emission was strongly inhibited by catecholamines, probably because of their effective scavenging of ROS. Based on this observation, a simple, rapid and sensitive new CL method was developed for the determination of catecholamines. The detection limits (3sigma) for dopamine, l-dopa, norepinephrine and epinephrine were 0.63, 1.37, 0.56 and 14.3 nmol/L, respectively. The linear range was 1-10 nmol/L; relative standard deviations were 0.71-1.34% for 0.1 micromol/mL catecholamines. This CL method was applied to the determination of catecholamines in pharmaceutical injections with satisfactory results.

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“…Several detection techniques in CE, including LIF [20][21][22], UV [23,24], MS [25], EC [26][27][28], and fluorescence [29], have been developed to determine catecholamines. Although LIF detection has the advantage of high sensitivity, the disadvantages are expensive instrumentation and complicated approach.…”

Section: Introductionmentioning

confidence: 99%

Determination of catecholamines by CE with direct chemiluminescence detection

Liu

Wang

et al. 2007

Electrophoresis

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A rapid and sensitive method to detect three catecholamines, isoprenaline, epinephrine, and dopamine, by CE coupled with direct luminol-potassium periodate chemiluminescence (CL) detection is described. The conditions for CE separation and CL reaction were systematically optimized. Under the optimum conditions, the baseline separation of three catecholamines was achieved within 6.5 min. The LODs obtained in standard solution were 5.3 6 10 -8 mol/L for isoprenaline, 4.7 6 10 -8 mol/L for epinephrine, and 1.5 6 10 -7 mol/L for dopamine. The RSD of the migration time and peak area were less than 1.8 and 3.6% (n = 5), respectively. The present method was applied to the determination of the dopamine in urine samples of cigarette smokers and nonsmokers. The results obtained indicate that there is a close relationship between the content of dopamine in human urine and the amount of cigarettes smoked daily; the level of dopamine in smokers is higher than in nonsmokers.

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Simultaneous determination of polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection

Cited by 74 publications

References 39 publications

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

Enhancement of chemiluminescence of the KIO₄–luminol system by gallic acid, acetaldehyde and Mn²⁺: application for the determination of catecholamines

Determination of catecholamines by CE with direct chemiluminescence detection

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Simultaneous determination of polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection

Cited by 74 publications

References 39 publications

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

Involvement of potD in Streptococcus pneumoniae Polyamine Transport and Pathogenesis

Enhancement of chemiluminescence of the KIO4–luminol system by gallic acid, acetaldehyde and Mn2+: application for the determination of catecholamines

Determination of catecholamines by CE with direct chemiluminescence detection

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Enhancement of chemiluminescence of the KIO₄–luminol system by gallic acid, acetaldehyde and Mn²⁺: application for the determination of catecholamines