Simultaneous determination of polythiazide and prazosin in tablets by second-order derivative UV spectroscopy

Validated specific HPLC methods for determination of prazosin, terazosin and doxazosin in the presence of degradation products formed under ICH-recommended stress conditions

“…For example, automated coulometric [6], spectrophotometric and HPLC [7] methods have been employed for assay of pure prazosin. Differential pulse polarographic [8,9], spectrophotometric [10][11][12][13][14], fluorometric [10], spectrofluorometric [13], voltammetric [14], colorimetric [15] and HPLC [16][17][18] methods have been proposed for determination of the same drug in different dosage forms.…”

A Spectrophotometric Method for the Determination of Prazosin Hydrochloride in Tablets

“…There are fluorimetric 6 , UV spectrophotometric 7,8 , GLC 9 , TLC 10 , voltametric 11,12 , and differential pulse polarographic 13−15 methods in the literature for the assay of the drug. The official USP XXII method uses a HPLC technique for the determination of 1 in capsules 5 .…”

Ultraviolet and light absorption spectrometry

“…The utilization of derivative spectroscopy in kinetic-based determinations has been studied and shown to provide improved selectivity (232). A number of papers report combining the techniques of derivative spectroscopy with simultaneous-multiwavelength measurements to determine aspirin and caffeine in pharmaceutical preparations (233), perphenazine-amitriptyline, chlordiazepoxide-amitriptyline, and trigluorperazine-isopropamide tranquilizerantidepressant mixtures (234), binary mixtures of phenazopyridine with ampicillin, nitrofurantoin, and sulfisoxazole in tablets (235), polythiazide and prazosin in tablets (236), and salicylic and salicyluric acids (237). The zero-, second-, and fourth-order derivatives have been studied with respect to their applicability for determining salbutamol sulfate in the presence of varying concentrations of gelatin (238).…”