Simultaneous Determination of Propylene Glycol, Glycerol and Lactic Acid in Rat Plasma and Serum by Gas Chromatography–mass Spectrometry

Liang, D; Gao, Yi Han; Zheng, Shengping; Li, Gang; Da, Wu; Shen, Yi

doi:10.1134/s1061934820020112

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…To 100 μl of serum samples, 10 μl of IS was added in a centrifuge tube then vortexed with 1 ml acetonitrile for 5 min. The mixture was thoroughly shaken and centrifuged at 13,000 rpm for 5 min (Liang et al, 2020). The resulting supernatant was reserved for analysis.…”

Section: Preparation Of Serum Samplesmentioning

confidence: 99%

Quantification of eucalyptol (1,8‐cineole) in rat serum by gas chromatography–mass/mass spectrometry and its application to a rat pharmacokinetic study

Liu

Dong

et al. 2021

Biomedical Chromatography

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Eucalyptol (1,8‐cineole) is a biologically active cyclic monoterpenoid. In a preliminary study, we used gas chromatography–mass spectrometry to detect eucalyptol in the serum and brain tissue of rats after oral administration. However, the absorption characteristics in vivo and pharmacokinetic parameters of eucalyptol have not been published to date. The present study aims to develop and validate a simple, sensitive GC–MS/MS method with quadrupole mass analyzer type for the quantitative analysis of eucalyptol in rat serum and apply it to a pharmacokinetic study. The assay showed linearity of concentration range from 50 to 5,000 pg/ml with a limit of quantitation of 50 pg/ml. Intra‐ and inter‐day precision for eucalyptol were 4.4–13.0 and <15.0%, respectively, and accuracy was within 10% for quality control samples. The recovery and stability results showed that the method was accurate and stable for quantitative analysis. The developed analytical method was successfully applied to a pharmacokinetic study after a single oral administration of eucalyptol in rat subjects. The serum concentration–time profiles indicate that the absorption characteristics of eucalyptol after oral administration are similar to those for intravenous administration.

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Section: Preparation Of Serum Samplesmentioning

confidence: 99%

Quantification of eucalyptol (1,8‐cineole) in rat serum by gas chromatography–mass/mass spectrometry and its application to a rat pharmacokinetic study

Liu

Dong

et al. 2021

Biomedical Chromatography

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“…2-HQ as a reactive matrix showed high reaction efficiency with a limit of detection (LOD) of 10 nM obtained for acetone and acetaldehyde (Figure S5). Citric acid (CA) and lactic acid (LA), important metabolites in the tricarboxylic acid cycle, have been detected in urine and serum samples, , respectively. Using 2-HQ as a matrix, CA and LA were detected by MALDI–MS in negative ion mode, with LOD below 1 nM (Figure S6).…”

Section: Resultsmentioning

confidence: 99%

Biofluid Metabolic Profiling for Lung Cancer Screening via Reactive Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Sun

Jia

et al. 2023

Anal. Chem.

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Lung cancer (LC) has the highest mortality rate among various cancer diseases. Developing an early screening method for LC with high classification accuracy is essential. Herein, 2-hydrazinoquinoline (2-HQ) is utilized as a dual-mode reactive matrix for metabolic fingerprint analysis and LC screening via matrix-assisted laser desorption ionization mass spectrometry (MALDI−MS). Metabolites in both positive mode and negative mode can be detected using 2-HQ as the matrix, and derivative analysis of aldehyde and ketone compounds can be achieved simultaneously. Hundreds of serum and urine samples from LC patients and healthy volunteers were analyzed. Combined with machine learning, LC patients and healthy volunteers were successfully distinguished with a high area under the curve value (0.996 for blind serum samples and 0.938 for urine). The MS signal was identified for metabolic profiling, and dysregulated metabolites of the LC group were analyzed. The above results showed that this method has great potential for rapid screening of LC.

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Liquid Chromatography with Orbitrap High-Resolution Mass Spectrometry for the Determination of Glucose in Saliva Following Derivatization with Dibenzylamine

Tang,

Wan,

Zhang

et al. 2024

Analytical Letters

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Simultaneous Determination of Propylene Glycol, Glycerol and Lactic Acid in Rat Plasma and Serum by Gas Chromatography–mass Spectrometry

Cited by 4 publications

References 33 publications

Quantification of eucalyptol (1,8‐cineole) in rat serum by gas chromatography–mass/mass spectrometry and its application to a rat pharmacokinetic study

Quantification of eucalyptol (1,8‐cineole) in rat serum by gas chromatography–mass/mass spectrometry and its application to a rat pharmacokinetic study

Biofluid Metabolic Profiling for Lung Cancer Screening via Reactive Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Liquid Chromatography with Orbitrap High-Resolution Mass Spectrometry for the Determination of Glucose in Saliva Following Derivatization with Dibenzylamine

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