Simultaneous determination of rosiglitazone and metformin in plasma by gradient liquid chromatography with UV detection

Yardımcı, Ceren; Özaltı́n, Nuran; Gürlek, Alper

doi:10.1016/j.talanta.2007.01.042

Cited by 36 publications

(28 citation statements)

References 34 publications

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“…The concentrations of drugs in plasma samples obtained from patients treated with glibenclamide, gliclazide, glimepiride, metformin, and vildagliptin are summarized in Table 4. These values are in agreement with the therapeutic range and plasma concentrations observed in previous studies [21–28, 31]. The mean plasma concentrations of the analytes observed in volunteers’ samples are summarized in Table 5.…”

Section: Resultssupporting

confidence: 90%

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Simultaneous Quantification of Antidiabetic Agents in Human Plasma by a UPLC–QToF-MS Method

et al. 2016

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An ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry method for the simultaneous quantification of chlorpropamide, glibenclamide, gliclazide, glimepiride, metformin, nateglinide, pioglitazone, rosiglitazone, and vildagliptin in human plasma was developed and validated, using isoniazid and sulfaquinoxaline as internal standards. Following plasma protein precipitation using acetonitrile with 1% formic acid, chromatographic separation was performed on a cyano column using gradient elution with water and acetonitrile, both containing 0.1% formic acid. Detection was performed in a quadrupole time-of-flight analyzer, using electrospray ionization operated in the positive mode. Data from validation studies demonstrated that the new method is highly sensitive, selective, precise (RSD < 10%), accurate (RE < 12%), linear (r > 0.99), free of matrix and has no residual effects. The developed method was successfully applied to volunteers’ plasma samples. Hence, this method was demonstrated to be appropriate for clinical monitoring of antidiabetic agents.

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Section: Resultssupporting

confidence: 90%

“…The range of the calibration curve was based on therapeutic range and plasma concentration of antidiabetic agents found in others studies in literature [20–31]. Eight concentration levels of analytes were prepared in triplicate from serial dilutions of working solutions of NAT, PIO, and ROS.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Quantification of Antidiabetic Agents in Human Plasma by a UPLC–QToF-MS Method

et al. 2016

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“…contained 7 different compounds. Therefore, in order to improve the separation, the technique of elution was changed to gradient elution system [19]. The first mobile phase composition in gradient elution (GRAD-1) was methanol/water with gradient increase methanol from 40-80% in 7 min to separate peaks 1 and 2 as well as peaks 4 and 5, followed by gradient increase methanol up to 90% from 7.01-15.00 min to speed up the elution process peaks 6 and 7.…”

Section: Results and Discussion Optimization Of Lc-uv Methodsmentioning

confidence: 99%

Liquid Chromatography for Analysis of Metformin in Myrmeleon sp.

Muadifah

Sulistyarti

Prasetyawan

2017

J. Pure App. Chem. Res.

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Myrmeleon sp. is typical of insect larva which has been used in Indonesia for diabetes treatment. However, there is no sufficient scientific report explaining the bioactive compounds in this insect. Based on our preliminary research, this insect contained metformin, i.e. one of bioactive compounds for the treatment of type-2 diabetes. Therefore, this study is focused on the development of separation technique using high-performance liquid chromatography (HPLC) on a reverse phase C-18 column with UV detection to identify and quantify metformin in methanol extract of Myrmeleon sp. Several parameters of HPLC method were optimized in accordance with the high resolution of separation and accurate determination of metformin. Satisfied separation was obtained under gradient elution mode using aqueous methanol/water with gradual increase of methanol from 50-90% and the flow rate of 0.5 mL/min with detection wavelength of 233 nm. The method performed total separation for all compounds in less than 11 min. Spiking technique was chosen for metformin identification and quantitation. Metformin in extract Myrmeleon sp. was eluted at the retention time (t R ) of 4.095 min, similarly with the retention time of standard metformin of 4.092 min. The quantity of metformin in Myrmeleon sp. can be simply determined by comparing the additional area of standard metformin and area of metformin from extract Myrmeleon sp. The results confirmed that the methanol extract of Myrmeleon sp. contained the anti-diabetes compound of metformin of 0.58 mg/g Myrmeleon sp. larvae with acceptable coefficient variation of 5.56 %.

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“…Several LC methods have been reported in the literature for the quantitative determination of RSG (the parent drug) in biological fluids 5–22 or in pharmaceutical formulations 23, 24. On the other hand, only Kim et al have described the simultaneous quantification of RSG, N ‐Dm‐R, and ρ‐OH‐R in biological matrix using mass spectrometry detection 16.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of rosiglitazone and its metabolites in rat liver microsomal fraction using hollow‐fiber liquid‐phase microextraction for sample preparation

Calixto

Bonato

2010

J of Separation Science

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A three-phase hollow-fiber liquid-phase microextraction method for the analysis of rosiglitazone and its metabolites N-desmethyl rosiglitazone and ρ-hydroxy rosiglitazone in microsomal preparations is described for the first time. The drug and metabolites HPLC determination was carried out using an X-Terra RP-18 column, at 22°C. The mobile phase was composed of water, acetonitrile and acetic acid (85:15:0.5, v/v/v) and the detection was performed at 245 nm. The hollow-fiber liquid-phase microextraction procedure was optimized using multifactorial experiments and the following optimal condition was established: sample agitation at 1750 rpm, extraction for 30 min, hydrochloric acid 0.01 mol/L as acceptor phase, 1-octanol as organic phase, and donor phase pH adjustment to 8.0. The recovery rates, obtained by using 1 mL of microsomal preparation, were 47-70%. The method presented LOQs of 50 ng/mL and it was linear over the concentration range of 50-6000 ng/mL, with correlation coefficients (r) higher than 0.9960, for all analytes. The validated method was employed to study the in vitro biotransformation of rosiglitazone using rat liver microsomal fraction.

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Simultaneous determination of rosiglitazone and metformin in plasma by gradient liquid chromatography with UV detection

Cited by 36 publications

References 34 publications

Simultaneous Quantification of Antidiabetic Agents in Human Plasma by a UPLC–QToF-MS Method

Simultaneous Quantification of Antidiabetic Agents in Human Plasma by a UPLC–QToF-MS Method

Liquid Chromatography for Analysis of Metformin in Myrmeleon sp.

Simultaneous determination of rosiglitazone and its metabolites in rat liver microsomal fraction using hollow‐fiber liquid‐phase microextraction for sample preparation

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