Simultaneous determination of sex hormones in egg products by ZnCl2 depositing lipid, solid-phase extraction and ultra performance liquid chromatography/electrospray ionization tandem mass spectrometry

Wang, Quanlin; Zhang, Aizhi; Xu, Ping; Chen, Liren

doi:10.1016/j.aca.2010.08.014

Cited by 38 publications

(28 citation statements)

References 21 publications

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“…Each sample was extracted twice in 1 ml methanol by vortexing, followed by centrifugation at 12,000 g for 10 min at room temperature. The supernatant was transferred to tubes containing 200 mg solid ZnCl 2 for lipid precipitation (Wang et al, 2010). The total volume of the combined supernatants was made to 4 ml by adding 2 ml methanol, and centrifuged at 12,000 g for 10 min at 4°C.…”

Section: Extraction Of Steroidsmentioning

confidence: 99%

Avian yolk androgens are metabolized instead of taken up by the embryo during the first days of incubation

Kumar

Dam

Permentier

et al. 2019

Journal of Experimental Biology

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Several studies show effects of yolk androgens in avian eggs on the phenotype of the offspring. Yolk hormone concentrations decline strongly within the first few days of incubation. Although early embryonic uptake of yolk androgens is suggested by the presence of radioactivity in the embryo when eggs are injected with radiolabelled androgens, these studies do not verify the chemical identity of radioactive compound(s), although it is known that these androgens can be metabolized substantially. By using stable isotopelabelled testosterone and androstenedione in combination with mass spectrometry, enabling verification of the exact molecular identity of labelled compounds in the embryo, we found that after 5 days of incubation the androgens were not taken up by the embryo. However, their concentrations in the entire yolk albumen homogenates declined strongly, even when corrected for dilution by albumen and water. Our results indicate metabolism of maternal androgens, very likely to 5βandrostane-3α,17β-diol, etiocholanolone and their conjugated forms. The results imply that the effects of increased exposure of the embryo to maternal androgens take place either before this early conversion or are mediated by these metabolites with an as yet unknown function, opening new avenues for understanding hormone-mediated maternal effects in vertebrates.

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Section: Extraction Of Steroidsmentioning

confidence: 99%

Avian yolk androgens are metabolized instead of taken up by the embryo during the first days of incubation

Kumar

Dam

Permentier

et al. 2019

Journal of Experimental Biology

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“…It should be remarked that these compounds can be easily ionized under soft conditions in the ESI despite the fact that they are generally uncharged in a wide pH range (Supporting Information Table S1). In fact, some works have previously determined them both in the negative [13,14] and positive mode [15,16]. For the suitable chromatographic separation of the 12 selected compounds, an X-Bridge C 18 100 mm x 4.6 mm x 3.5 m column and a 20 mm x 4.6 mm x 3.5 m pre-column filled with the same stationary phase, both end-capped, were used.…”

Section: Optimization Of the Separation And Detection Conditionsmentioning

confidence: 99%

Evaluation of two molecularly imprinted polymers for the solid‐phase extraction of natural, synthetic and mycoestrogens from environmental water samples before liquid chromatography with mass spectrometry

González‐Sálamo

Socas‐Rodríguez

Hernández‐Borges

et al. 2015

J of Separation Science

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In this work, we have compared the selectivity of two commercial molecularly imprinted polymers (AFFINIMIP®SPE Estrogens and AFFINIMIP®SPE Zearalenone) for the extraction of 12 estrogenic compounds of interest (i.e. 17α-estradiol, 17β-estradiol, estrone, hexestrol, 17α-ethynylestradiol, diethylstibestrol, dienestrol, zearalenone, α-zearalanol, β-zearalanol, α-zearalenol and β-zearalenol) from different water samples. High-performance liquid chromatography coupled with ion trap mass spectrometry with electrospray ionization was used for their determination. Results showed that although both molecularly imprinted polymeric cartridges were specifically designed for different groups of analytes (natural estrogens like estradiol in the first case and zearalenone derivatives in the second) they nearly have the same extraction performance (with recovery values in the range 65-101%) for the same analytes in Milli-Q water because of the cross-reactivity of the polymer. However, when more complex water samples were analyzed, it was clear that the behavior was different and that the AFFINIMIP®SPE Estrogens showed less cross-reactivity than the other cartridge. Validation of the proposed methodology with both cartridges revealed that the extraction was reproducible and that the final limits of detection of the proposed method were in the low ng/L range.

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“…Estrogens and androgens are mostly analyzed separately [29][30][31][40][41][42][43][44] and a few of studies refer to simultaneous determination of them [27,[45][46][47][48][49]. Our work attempted to simultaneously detect estrogenic and androgenic hormones of different polarities.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of five estrogens and four androgens in water samples by online solid-phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry

Guo

Liu

et al. 2013

Journal of Chromatography A

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a b s t r a c tA novel method for simultaneous determination of five estrogens and four androgens by online solid-phase extraction (SPE) coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-ESI-MS/MS) in water samples was developed. An aliquot of 50 mL water sample after filtration was injected directly into autosampler and the analytes were preconcentrated on a NG1 online SPE column. After cleanup step the analytes were eluted in back flush mode and then separated on a liquid chromatography column. The experimental parameters, such as sample loading flow rate, cleanup condition and elution time, were optimized in detail. Estrogens and androgens were detected in negative and positive mode, respectively. High ionization efficiency of all the analytes was achieved by adding of 1‰ ammonia in the mobile phase. The recoveries ranged from 31.8% to 119.0% and the inter-day RSDs ranged from 2.7% to 19.6%. The limits of detections (LODs) were between 0.1 and 2.5 ng/L. The proposed method was successfully applied to the analysis of three types of water samples, including river water, influent and effluent water from a wastewater treatment plant (WWTP). The recoveries of androgens were not that good and a further study is being planned to improve the sensitivity for them. The proposed method is simple, sensitive and suitable for simultaneous analysis and monitoring of estrogens and androgens in water samples.

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Simultaneous determination of sex hormones in egg products by ZnCl2 depositing lipid, solid-phase extraction and ultra performance liquid chromatography/electrospray ionization tandem mass spectrometry

Cited by 38 publications

References 21 publications

Avian yolk androgens are metabolized instead of taken up by the embryo during the first days of incubation

Avian yolk androgens are metabolized instead of taken up by the embryo during the first days of incubation

Evaluation of two molecularly imprinted polymers for the solid‐phase extraction of natural, synthetic and mycoestrogens from environmental water samples before liquid chromatography with mass spectrometry

Simultaneous determination of five estrogens and four androgens in water samples by online solid-phase extraction coupled with high-performance liquid chromatography–tandem mass spectrometry

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