Simultaneous determination of sulfamethoxazole and trimethoprim in biological fluids for high-throughput analysis: Comparison of HPLC with ultraviolet and tandem mass spectrometric detection

Bedor, Danilo César Galindo; Gonçalves, Talita Mota; Ferreira, Maria Luz; Sousa, Carlos Eduardo Miranda de; Menezes, A.L.; Oliveira, Elder Eldervitch Carneiro de; Santana, Davi Pereira de

doi:10.1016/j.jchromb.2007.12.027

Cited by 65 publications

(34 citation statements)

References 30 publications

(40 reference statements)

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“…The results presented in Table ( 8) indicate that no significant interferences were found from any of the studied excipients in determination of SMZ except vanillin that completely interferes.…”

Section: Interference Studymentioning

confidence: 82%

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Spectrophotometric Determination of Sulfamethoxazole Based on Charge-Transfer Complexation with Sodium Nitroprusside

Mahmoud¹,

Dikran²,

Mohammed³

2017

IHJPAS

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A simple, accurate and precise spectrophotometric method has been developed for the analysis of sulfamethoxazole (SMZ) in pure form and pharmaceutical preparation. The method involves a direct charge transfer complexation of sulfamethoxazole (SMZ) with sodium nitroprusside (SNP) in alkaline medium and the presence of hydroxyl amine hydrochloride. Variables affecting the formation of the formed orange colored complex were optimized following two approaches univariate and central composite experimental design (CCD) multivariate. Under optimum recommended conditions, the formed complex exhibits λmax at 512 nm and the method conforms Beer's law for SMZ concentration in the range of 5.0-150.0 (µg.mL -1 ) with molar absorptivity 1.139×10 3 L.mol -1 .cm -1 , and r = 0.9997. Analysis of SMZ pharmaceutical dosages shows a good agreement with the real amounts.

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Section: Interference Studymentioning

confidence: 82%

“…The literature contains various methods for the determination of sulfamethoxazole in its pharmaceutical formulations. This involves spectrophotometric [2][3][4][5][6][7], HPLC [8][9][10][11] [20] methods. The structural formula of SMZ is shown in Scheme (1).…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Sulfamethoxazole Based on Charge-Transfer Complexation with Sodium Nitroprusside

Mahmoud¹,

Dikran²,

Mohammed³

2017

IHJPAS

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“…Although as compared to mass spectrometry less sophisticated analysis is performed with the use of the other conventional detectors, the speed and sensitivity of the former one surpass those offered by the latter ones (UV, CL, EC). The current interest focuses on LC-MS/MS [60,[64][65][66][67][68][69][70][71][72][73][74]. This is not surprising as it is well acknowledged that LC-MS/ MS has the power of combining the sensitivity of tandem MS and the selectivity of chromatography thus improving the accuracy of quantitating low drug concentrations from different matrices.…”

Section: Overview Of Published Analytical Methods Optimized For Theramentioning

confidence: 99%

“…Although different analytical methods have been developed for the quantification of individual antibiotics, in the last 5 years several methods have been published for the simultaneous analysis of subsets of antibiotics usually given to critically ill patients [64][65][66][67][68][69][70][71][72]74], and continuous attempts for improved method development are ongoing. This stems from the need for rapid and accurate methods for the detection in one run of as many as possible of the antibiotics used as first and second lines of treatment in critically ill patients.…”

Section: Lc-ms/ms For Antibiotics Therapeutic Monitoring What Shall Wmentioning

confidence: 99%

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

El‐Najjar

Gessner

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Cancer remains a leading cause of mortality and morbidity worldwide. In addition to organ failure, the most frequent reasons for admission of cancer patients to intensive care units (ICU) are: infections and sepsis. As critically ill, the complexity of the health situation of cancer patients renders the standard antimicrobial regimen more complex and even inadequate which results in increased mortality rates. This is due to pathophysiological changes in the volume of distribution, increased clearance, as well as to organ dysfunction. While in the former cases a decrease in drug efficacy is observed, the hallmark of the latter one is overdosing leading to increased toxicity at the expense of efficacy. Furthermore, an additional risk factor is the potential drug-drug interaction between antibiotics and antineoplastic agents. Therefore, therapeutic drug monitoring (TDM) is a necessity to improve the clinical outcome of antimicrobial therapy in cancer patients. To be applied in routine analysis the method used for TDM should be cheap, fast and highly accurate/sensitive. Furthermore, as ICU patients are treated with a cocktail of antibiotics the method has to cover the simultaneous analysis of antibiotics used as a first/second line of treatment. The aim of the current review is to briefly survey the pitfalls in the current antimicrobial therapy and the central role of TDM in dose adjustment and drug-drug interaction's evaluation. A major section is dedicated to summarize the currently published analytical methods and to shed light on the difficulties and potential problems that can be encountered during method development.

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“…Several extraction methods have been used for SA-residue analysis; however, most are long and tedious, involving liquid-liquid extraction (LLE) (Haller et al, 2002;Chico et al, 2008), or solid-phase extraction (SPE) (Pecorelli et al, 2004;Koesukwiwat, Jayanta, Leepipatpiboon, 2007;Bedor et al, 2008), which also include an additional step to precipitate the proteins. In many instances, LLE and SPE were used in combination; for example, after deproteinization/analyte extraction by means of a suitable organic solvent and solvent removal, the extracts were subsequently purified using suitable SPE procedures (Biswas et al, 2007;Soto-Chinchilla, García-Campaña, Gamiz-Gracia, 2007;Gamba et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

QuEChERS-HPLC-DAD method for sulphonamides in chicken breast

Machado

Landin-Silva

Maia

et al. 2013

Braz. J. Pharm. Sci.

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The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 µm) at 40°C, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min -1 , increased by 1.2 mL min -1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 µg kg -1 , precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and 1.9-10.9 (SMX) and interday precision (RSD%) was between 1.5-9.7, 1.7-4.1 and 2.1-10.2, respectively. Results of accuracy (bias) were in the range of -8.6 to +11.9 %. Therefore, the validated method is clearly useful for the practical residue monitoring of the drugs evaluated in chicken samples, as all the values were within the acceptable criteria used for food safety. Of 6 samples analyzed, none of them showed contamination of the sulphonamides studied at detectable levels. , aumentando 1,2 mL min -1 e a 265 nm é apresentado para a determinação simultânea de sulfadiazina, sulfametoxipiridazina e sulfametoxazol em amostras de peito de frango. O QuEChERS é barato, rápido e fácil, e a extração dos analitos da matriz foi empregada com sucesso. Além disso, o método apresentou linearidade, na faixa de 25, 50, 100, 150, 175 e 200 µg kg -1 , precisão, seletividade e sensibilidade. A precisão intradia (RSD %) para o método QuEChERS foi entre 3,6-10,8 (SDZ), 6,9-14,1 (SPZ) e 1,9-10,9 (SMX) e a precisão interdias (RSD%) foi entre 1,5-9,7, 1,7-4,1 e 2,1-10,1, respectivamente. Resultados de exatidão (tendenciosidade) foram na faixa de -8,6 a +11,9%. Portanto, o método validado é útil para a monitorização de resíduos de medicamentos avaliados em amostras de frangos, bem como todos os valores estavam dentro dos critérios aceitáveis utilizados para a segurança dos alimentos. De seis amostras analisadas, nenhuma apresentou contaminação de sulfonamidas nos níveis detectáveis estudados.

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Simultaneous determination of sulfamethoxazole and trimethoprim in biological fluids for high-throughput analysis: Comparison of HPLC with ultraviolet and tandem mass spectrometric detection

Cited by 65 publications

References 30 publications

Spectrophotometric Determination of Sulfamethoxazole Based on Charge-Transfer Complexation with Sodium Nitroprusside

Spectrophotometric Determination of Sulfamethoxazole Based on Charge-Transfer Complexation with Sodium Nitroprusside

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

QuEChERS-HPLC-DAD method for sulphonamides in chicken breast

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